Expression profile of toll-like receptors within the gastrointestinal tract of 2-day-old Salmonella enteriditis-infected broiler chickens

MacKinnon, Kathryn M.; He, Haiqi; Nerren, Jessica R.; Swaggerty, Christina L.; Genovese, Kenneth J.; Kogut, Michael H.

doi:10.1016/j.vetmic.2009.01.024

Cited by 68 publications

(44 citation statements)

References 20 publications

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“…This receptor cooperates with CD14 to mediate the innate immune response to bacterial lipopolysaccharide (LPS) leading to NFkappa-B activation, cytokine secretion and the inflammatory response. TLR-4 is typically increased after most microbial infections (Mackinnon et al, 2009;Abasht et al, 2008). Its downregulation by Orego-Stim indicates that Orego-Stim may suppress TLR-4 mediated inflammatory pathways in the ceacal tonsils.…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Non-Antibiotic Alternatives in Coccidia Challenged Broiler Chickens

Adedokun

Adeola

et al. 2014

J. Poult. Sci.

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This study was conducted to determine the effect of selected antibiotic alternatives on growth performance and inflammatory gene expression in broiler chickens challenged with Eimeria oocysts from Coccivac ® vaccine. A randomized complete block design with 6 treatments and 8 replicate pens per treatment was used. The treatments were; negative control (NC) with neither antibiotic nor antibiotic alternatives, Salinomycin positive control (PC), essential oil (Orego-Stim

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Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory Effects of Non-Antibiotic Alternatives in Coccidia Challenged Broiler Chickens

Adedokun

Adeola

et al. 2014

J. Poult. Sci.

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“…For chickens, the influence of Salmonella infection on TLR4 mRNA expression has been studied mainly at the organ level, in the ceca and spleen, but with conflicting results. In some experiments, TLR4 expression in the ceca increased after infection (1,27,33), while others found no change (19,26). Shaughnessy et al (36) found that TLR4 expression increased at 6 h postinfection (p.i.…”

Section: Discussionmentioning

confidence: 99%

“…Variation of TLR4 has been analyzed in vitro after incubation of peritoneal macrophages (14), ovarian follicles (37), and thrombocytes (35) with LPS and in heterophils in response to S. Enteritidis (30,31). On the other hand, its expression has been measured during the acute phase in Salmonella-infected chickens, in whole ceca or spleens (1,19,26,27,34,36) and in peripheral blood lymphocytes (28). Only one study has investigated the role of TLR4 during the carrier state in adult hens (33).…”

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confidence: 99%

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Expression of Toll-Like Receptor 4 and Downstream Effectors in Selected Cecal Cell Subpopulations of Chicks Resistant or Susceptible to Salmonella Carrier State

Chaussé¹,

Grépinet²,

Bottreau³

et al. 2011

Infect Immun

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Toll-like receptor 4 (TLR4), which recognizes lipopolysaccharide from Gram-negative bacteria, plays a major role in resistance of mice and humans to Salmonella infection. In chickens, Salmonella may establish a carrier state whereby bacteria are able to persist in the host organism without triggering clinical signs. Based on cellular morphological parameters, we developed a method, without using antibodies, to separate three cecal cell subpopulations: lymphocytes, enterocytes, and a population encompassing multiple cell types. We analyzed the mRNA expression of TLR4, interleukin-1␤ (IL-1␤), IL-8, IL-12, and lipopolysaccharide-induced tumor necrosis factor alpha factor (LITAF) in cecal subpopulations of chicks from inbred lines resistant or susceptible to the carrier state infected with Salmonella enterica serovar Enteritidis. The results showed that resistance to the carrier state in chicks is associated with a larger percentage of lymphocytes and with higher levels of expression of TLR4 and IL-8 at homeostasis in the three cell subpopulations, as well as with a higher level of expression of LITAF in lymphocytes during the carrier state. In contrast to the early phase of infection, the carrier state is characterized by no major cell recruitment differences between infected and noninfected animals and no significant modification in terms of TLR4, IL-1␤, IL-8, IL-12, and LITAF expression in all cell subpopulations measured. However, TLR4 expression increased in the lymphocytes of chicks from the susceptible line, reaching the same level as that in infected chicks from the resistant line. These observations suggest that the carrier state is characterized by a lack of immune activation and highlight the interest of working at the level of the cell population rather than that of the organ.

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“…Pattern recognition receptors, including TLRs, are important parts of the innate immune system, particularly in the first week post hatching (Bar-Shira et al, 2003). The expression of TLRs in the broiler caecal tonsil increased significantly 2 days after inoculation with S. Enteritidis (MacKinnon et al, 2009). A major effect of the TLR-4 polymorphism on the resistance of chickens to S. Typhimurium was observed 7 days after inoculation with the bacteria .…”

Section: Cd44mentioning

confidence: 93%

The effects of polymorphisms inIL-2,IFN-γ,TGF-β2,IgL,TLR-4,MD-2, andiNOSgenes on resistance toSalmonellaEnteritidis in indigenous chickens

et al. 2012

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Expression profile of toll-like receptors within the gastrointestinal tract of 2-day-old Salmonella enteriditis-infected broiler chickens

Cited by 68 publications

References 20 publications

Anti-Inflammatory Effects of Non-Antibiotic Alternatives in Coccidia Challenged Broiler Chickens

Anti-Inflammatory Effects of Non-Antibiotic Alternatives in Coccidia Challenged Broiler Chickens

Expression of Toll-Like Receptor 4 and Downstream Effectors in Selected Cecal Cell Subpopulations of Chicks Resistant or Susceptible to Salmonella Carrier State

The effects of polymorphisms inIL-2,IFN-γ,TGF-β2,IgL,TLR-4,MD-2, andiNOSgenes on resistance toSalmonellaEnteritidis in indigenous chickens

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