Expression profile of the SOX9 gene in the testes of sexually immature and mature male goats (Capra hircus), and its potential influence on postnatal testis development

Szatkowska, Iwona; Jędrzejczak-Silicka, Magdalena; Dybus, Andrzej; Wiszniewska, Barbara; Udała, J.; Zaborski, Daniel; Wójcik, Janusz; Stankiewicz, Tomasz; Proskura, Witold Stanisław

doi:10.17221/147/2016-vetmed

Cited by 4 publications

(2 citation statements)

References 17 publications

(29 reference statements)

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“…Real-time quantitative RT-qPCR was performed using SYBR Green PCR Master Mix (KAPA™ SYBR ® qPCR Kit, KapaBiosystems, Woburn, MA) according to the manufacturer instructions. The primers used for expression were already reported ( GAPDH, β-Actin, CSN-2, TLR4, TRIF, MyD88, IRF3, TRAF3, MAPK1, TNF-α, IL-8, NF-kB, TRAF6 and INF-β) [ 28 , 50 – 54 ]. SCARB1 primers were designed using Primer3 Plus software.…”

Section: Methodsmentioning

confidence: 99%

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

et al. 2023

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SCARB1 belongs to class B of Scavenger receptors (SRs) that are known to be involved in binding and endocytosis of various pathogens. SRs have emerging role in regulating innate immunity and host–pathogen interactions by acting in co-ordination with Toll-like receptors.Query Little is known about the function of SCARB1 in milk-derived mammary epithelial cells (MECs). This study reports the role of SCARB1 in infection and its potential association in TLR4 signaling on bacterial challenge in Goat mammary epithelial cells (GMECs). The novelty in the establishment of MEC culture lies in the method that aims to enhance the viability of the cells with intact characteristics upto a higher passage number. We represent MEC culture to be used as a potential infection model for deeper understanding of animal physiology especially around the mammary gland. On E.coli challenge the expression of SCARB1 was significant in induced GMECs at 6 h. Endoribonuclease-esiRNA based silencing of SCARB1 affects the expression of TLR4 and its pathways i.e. MyD88 and TRIF pathways on infection. Knockdown also affected the endocytosis of E.coli in GMECs demonstrating that E.coli uses SCARB1 function to gain entry in cells. Furthermore, we predict 3 unique protein structures of uncharacterized SCARB1 (Capra hircus) protein. Overall, we highlight SCARB1 as a main participant in host defence and its function in antibacterial advances to check mammary gland infections.

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Section: Methodsmentioning

confidence: 99%

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

et al. 2023

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“…Real-time quantitative RT-qPCR was performed using SYBR Green PCR Master Mix (KAPA™ SYBR® qPCR Kit, KapaBiosystems, Woburn, MA) according to the manufacturer instructions. The primers used for expression were already reported ( GAPDH, β-Actin, CSN-2, TLR4, TRIF, MyD88, IRF3, TRAF3, MAPK1, TNF-α, IL-8, NF-kB, TRAF6 and INF-β) (28, 50, 51, 52, 53, 54). SCARB1 primers were designed using Primer3 Plus software.…”

Section: Methodsmentioning

confidence: 99%

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Taban

Ahmad

Mumtaz

et al. 2022

Preprint

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SCARB1 belongs to class B of Scavenger receptors (SRs) that are known to be involved in binding and endocytosis of various pathogens. SRs have emerging role in regulating innate immunity and host-pathogen interactions by acting in co-ordination with Toll-like receptors. Little is known about the function of SCARB1 in milk-derived mammary epithelial cells (MECs). This study reports the role of SCARB1 in infection and its potential association in TLR4 signaling on bacterial challenge in Goat mammary epithelial cells (GMECs). The novelty in the establishment of MEC culture lies in the method that aims to enhance the viability of the cells with intact characteristics upto a higher passage number. We represent MEC culture to be used as a potential infection model for deeper understanding of animal physiology especially around the mammary gland. On E.coli challenge the expression of SCARB1 was significant in induced GMECs at 6 h. Endoribonuclease- esiRNA based silencing of SCARB1 affects the expression of TLR4 and its pathways i.e. MyD88 and TRIF pathways on infection. Knockdown also affected the endocytosis of E.coli in GMECs demonstrating that E.coli uses SCARB1 function to gain entry in cells. Furthermore, we predict 3 unique protein structures of uncharacterized SCARB1 (Capra hircus) protein. Overall, we highlight SCARB1 as a main participant in host defence and its function in antibacterial advances to check mammary gland infections.

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Keçilerde puberta ve eşeysel olgunluk yaşı

Demir

Elmaz

Cerit

2018

Uludağ Üniversitesi Veteriner Fakültesi Dergisi

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Günümüzde değişen iklim koşullarına uyum sağlayan en önemli çiftlik hayvanlarından birisi keçi türüdür. Keçi yetiştiriciliğinde döl, süt ve et verimi önem taşır. Dölverimi tüm diğer verimlerin temeli olup et verimini doğrudan etkilemektedir. Üreme organlarının gelişmesi ile genç hayvanlarda üreme faaliyetlerinin başladıgı döneme puberta denir. Bu dönemde genç erkeklerin ejakulatında ilk spermatozoid, genç dişilerde ilk ovulasyon meydana gelir fakat hayvanlar cinsel olgunluğa tam ulaşmış değildir. Hayvanlar eşeysel olgunluğa ulaşır ulaşmaz hemen damızlıkta kullanılmazlar. Damızlıkta kullanma daha ileri yaşlarda olur. Böylece eşeysel olgunluk yaşı ile ilk defa damızlıkta kullanma yaşı farklı şeylerdir. Keçilerde yaşam boyu verim performansının belirlenmesinde önemli faktörlerden birisi puberta yaşıdır. Besleme, canlı ağırlık, ırk, doğum mevsimi ve büyüme oranı gibi birçok faktörün puberta yaşını etkilediği bilinmektedir. Bu derleme; dünyanın farklı bölgelerinde farklı ırklara ait erkek ve dişi keçilerde puberta yaşını etkileyen faktörler ile puperta yaşının ıslah çalışmaları açısından önemi hakkında detaylı bilgilerin verilmesi amaçlanmıştır.

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Expression profile of the SOX9 gene in the testes of sexually immature and mature male goats (Capra hircus), and its potential influence on postnatal testis development

Cited by 4 publications

References 17 publications

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Scavenger receptor B1 facilitates the endocytosis of Escherichia coli via TLR4 signaling in mammary gland infection

Keçilerde puberta ve eşeysel olgunluk yaşı

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