Expression Profile of Multidrug Resistance Efflux Pumps During Intracellular Life of Adherent-Invasive Escherichia coli Strain LF82

Fanelli, Giulia; Pasqua, Martina; Colonna, Bianca; Prosseda, Gianni; Grossi, Milena

doi:10.3389/fmicb.2020.01935

Cited by 14 publications

(11 citation statements)

References 47 publications

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“…DH10b was used as recipient strain in cloning procedures. Plasmid DNA extraction, DNA transformation, cloning, restriction, electrophoresis and purification of DNA fragments were performed as previously described [ 34 , 35 ]. PCR reactions were routinely performed using DreamTaq DNA polymerase (Thermo Fischer Scientific, Waltham, MA, USA).…”

Section: Methodsmentioning

confidence: 99%

Modulation of OMV Production by the Lysis Module of the DLP12 Defective Prophage of Escherichia coli K12

et al. 2021

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Outer membrane vesicles (OMVs) are nanostructures mostly produced by blebbing of the outer membrane in Gram negative bacteria. They contain biologically active proteins and perform a variety of processes. OMV production is also a typical response to events inducing stress in the bacterial envelope. In these cases, hypervesiculation is regarded as a strategy to avoid the dangerous accumulation of undesired products within the periplasm. Several housekeeping genes influence the biogenesis of OMVs, including those correlated with peptidoglycan and cell wall dynamics. In this work, we have investigated the relationship between OMV production and the lysis module of the E. coli DLP12 cryptic prophage. This module is an operon encoding a holin, an endolysin and two spannins, and is known to be involved in cell wall maintenance. We find that deleting the lysis module increases OMV production, suggesting that during evolution this operon has been domesticated to regulate vesiculation, likely through the elimination of non-recyclable peptidoglycan fragments. We also show that the expression of the lysis module is negatively regulated by environmental stress stimuli as high osmolarity, low pH and low temperature. Our data further highlight how defective prophages finely contribute to bacterial host fitness.

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Section: Methodsmentioning

confidence: 99%

Modulation of OMV Production by the Lysis Module of the DLP12 Defective Prophage of Escherichia coli K12

et al. 2021

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“…Further studies will be necessary to clarify whether EvgS/EvgA will induce the expression of MdtEF in Shigella species to enforce intracellular survival. Recently, using Adherent Invasive E. coli (AIEC) strain LF82 as a model, it was reported that EvgS/EvgA and PhoQ/PhoP TCS contribute to the intracellular survival of AIEC strains in macrophages [ 120 ] and that the MdtEF efflux pump is induced in macrophages and increases the bacterial fitness in this harsh environment [ 121 ].…”

Section: Role Of Evgs/evga During Intracellular Life Of Shi...mentioning

confidence: 99%

Roles of Two-Component Signal Transduction Systems in Shigella Virulence

et al. 2022

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Two-component signal transduction systems (TCSs) are widespread types of protein machinery, typically consisting of a histidine kinase membrane sensor and a cytoplasmic transcriptional regulator that can sense and respond to environmental signals. TCSs are responsible for modulating genes involved in a multitude of bacterial functions, including cell division, motility, differentiation, biofilm formation, antibiotic resistance, and virulence. Pathogenic bacteria exploit the capabilities of TCSs to reprogram gene expression according to the different niches they encounter during host infection. This review focuses on the role of TCSs in regulating the virulence phenotype of Shigella, an intracellular pathogen responsible for severe human enteric syndrome. The pathogenicity of Shigella is the result of the complex action of a wide number of virulence determinants located on the chromosome and on a large virulence plasmid. In particular, we will discuss how five TCSs, EnvZ/OmpR, CpxA/CpxR, ArcB/ArcA, PhoQ/PhoP, and EvgS/EvgA, contribute to linking environmental stimuli to the expression of genes related to virulence and fitness within the host. Considering the relevance of TCSs in the expression of virulence in pathogenic bacteria, the identification of drugs that inhibit TCS function may represent a promising approach to combat bacterial infections.

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“…The crucial role of the EvgA/EvgS TCS in activating the EPs required for a successful interaction with host cells is further highlighted by the analysis of the expression profile of MDR EPs in adherent-invasive Escherichia coli (AIEC) ( Fanelli et al, 2020 ). Similarly to Shigella, emrK expression in LF82, a strain isolated from Crohn disease lesions, is induced inside macrophages.…”

Section: The Efflux Pump Emrky Of Shigella Flexneri : the Crucial Role Of Two Component Systems In Sensing And Respondmentioning

confidence: 99%

“…The expression of mdtEF , which encodes a RND EP, is induced in AIEC cells inside macrophages but not inside epithelial cells. MdtEF is known to contribute to the survival of AIECs inside macrophages ( Fanelli et al, 2020 ). As shown in Figure 3 , mdtEF is co-transcribed with gadE as a gadE-mdtEF operon.…”

Section: The Efflux Pump Emrky Of Shigella Flexneri : the Crucial Role Of Two Component Systems In Sensing And Respondmentioning

confidence: 99%

Host - Bacterial Pathogen Communication: The Wily Role of the Multidrug Efflux Pumps of the MFS Family

Pasqua

Patti

Fanelli

et al. 2021

Front. Mol. Biosci.

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Bacterial pathogens are able to survive within diverse habitats. The dynamic adaptation to the surroundings depends on their ability to sense environmental variations and to respond in an appropriate manner. This involves, among others, the activation of various cell-to-cell communication strategies. The capability of the bacterial cells to rapidly and co-ordinately set up an interplay with the host cells and/or with other bacteria facilitates their survival in the new niche. Efflux pumps are ubiquitous transmembrane transporters, able to extrude a large set of different molecules. They are strongly implicated in antibiotic resistance since they are able to efficiently expel most of the clinically relevant antibiotics from the bacterial cytoplasm. Besides antibiotic resistance, multidrug efflux pumps take part in several important processes of bacterial cell physiology, including cell to cell communication, and contribute to increase the virulence potential of several bacterial pathogens. Here, we focus on the structural and functional role of multidrug efflux pumps belonging to the Major Facilitator Superfamily (MFS), the largest family of transporters, highlighting their involvement in the colonization of host cells, in virulence and in biofilm formation. We will offer an overview on how MFS multidrug transporters contribute to bacterial survival, adaptation and pathogenicity through the export of diverse molecules. This will be done by presenting the functions of several relevant MFS multidrug efflux pumps in human life-threatening bacterial pathogens as Staphylococcus aureus, Listeria monocytogenes, Klebsiella pneumoniae, Shigella/E. coli, Acinetobacter baumannii.

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Expression Profile of Multidrug Resistance Efflux Pumps During Intracellular Life of Adherent-Invasive Escherichia coli Strain LF82

Cited by 14 publications

References 47 publications

Modulation of OMV Production by the Lysis Module of the DLP12 Defective Prophage of Escherichia coli K12

Modulation of OMV Production by the Lysis Module of the DLP12 Defective Prophage of Escherichia coli K12

Roles of Two-Component Signal Transduction Systems in Shigella Virulence

Host - Bacterial Pathogen Communication: The Wily Role of the Multidrug Efflux Pumps of the MFS Family

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