Expression profile of microRNAs in c-Myc induced mouse mammary tumors

Sun, Yuan; Wu, Jack; Wu, Si Hung; Thakur, Archana; Bollig, Aliccia; Huang, Yong; Liao, D. Joshua

doi:10.1007/s10549-008-0171-6

Cited by 74 publications

(68 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Interestingly, weak miR-20b expression can be useful for predicting clinical behavior, enabling a group of MCLs with higher survival probability to be distinguished. MiR-20b expression has been found to have a role in other type of cancers, [61][62][63] in which its high level of expression was associated with a worse prognosis, as is the case for what we found in MCL. It should be noted that miR-20b is localized in a cluster (X chromosome) that shares some similarities with oncomir-1, which is already known to be strongly expressed in MCL cell lines, and is homologous at 21 out of 23 nucleotides with miR-20a, a member of the aforementioned cluster.…”

Section: Discussionsupporting

confidence: 75%

Mantle cell lymphoma: transcriptional regulation by microRNAs

et al. 2010

View full text Add to dashboard Cite

Mantle cell lymphoma (MCL) pathogenesis is still partially unexplained. We investigate the importance of microRNA (miRNA) expression as an additional feature that influences MCL pathway deregulation and may be useful for predicting patient outcome. Twenty-three MCL samples, eight cell lines and appropriate controls were screened for their miRNAs and gene expression profiles and DNA copy-number changes. MCL patients exhibit a characteristic signature that includes 117 miRNA (false discovery rate o0.05). Combined analysis of miRNAs and the gene expression profile, paired with bioinformatics target prediction (miRBase and TargetScan), revealed a series of genes and pathways potentially targeted by a small number of miRNAs, including essential pathways for lymphoma survival such as CD40, mitogen-activated protein kinase and NF-jB. Functional validation in MCL cell lines demonstrated NF-jB subunit nuclear translocation to be regulated by the expression of miR-26a. The expression of 12 selected miRNAs was studied by quantitative PCR in an additional series of 54 MCL cases. Univariate analysis identified a single miRNA, miR20b, whose lack of expression distinguished cases with a survival probability of 56% at 60 months. In summary, using a novel bioinformatics approach, this study identified miRNA changes that contribute to MCL pathogenesis and markers of potential utility in MCL diagnosis and clinical prognostication.

show abstract

Section: Discussionsupporting

confidence: 75%

Mantle cell lymphoma: transcriptional regulation by microRNAs

et al. 2010

View full text Add to dashboard Cite

show abstract

“…MALAT1 is required for the G1-S transition and mitotic progression via the regulation of cell cycle gene expression [196]. Although high MALAT1 expression is associated with poorer outcomes in a number of cancer types [197], it is downregulated in mammary tumors in c-myc transgenic mice [198]. However, the exact role of MALAT1 in breast cancer is still uncertain at this stage, as another line of investigation found that MALAT1 is upregulated in primary breast cancer [199].…”

Section: Nuclear Architectural Rnasmentioning

confidence: 99%

Noncoding RNAs in breast cancer

Lo¹,

Wolfson²,

Zhou³

et al. 2015

Briefings in Functional Genomics

View full text Add to dashboard Cite

The mammalian transcriptome has recently been revealed to encompass a large number of noncoding RNAs (ncRNAs) that play a variety of important regulatory roles in gene expression and other biological processes. MicroRNAs (miRNAs), the best studied of the short noncoding RNAs (sncRNAs), have been extensively characterized with regard to their biogenesis, function and importance in tumorigenesis. Another class of sncRNAs called piwi-interacting RNAs (piRNAs) has also gained attention recently in cancer research owing to their critical role in stem cell regulation. Long noncoding RNAs (lncRNAs) of >200 nucleotides in length have recently emerged as key regulators of developmental processes, including mammary gland development. lncRNA dysregulation has also been implicated in the development of various cancers, including breast cancer. In this review, we describe and discuss the roles of sncRNAs (including miRNAs and piRNAs) and lncRNAs in the initiation and progression of breast tumorigenesis, with a focus on outlining the molecular mechanisms of oncogenic and tumor-suppressor ncRNAs. Moreover, the current and potential future applications of ncRNAs to clinical breast cancer research are also discussed, with an emphasis on ncRNA-based diagnosis, prognosis and future therapeutics.

show abstract

“…[3][4][5] Subsequently, miRNAs have been shown to have important roles in many physiological processes of mammalian systems by influencing cell apoptosis, proliferation, differentiation, development, and metabolism through regulation of critical signaling molecules including cytokines, growth factors, transcription factors, and pro-apoptotic and anti-apoptotic proteins. [6][7][8] Increasing number of miRNAs have been identified in the human genome and they are collectively called the miRNome. 9 Accumulating evidence shows the potential involvement of altered regulation of miRNAs in initiation and progression in a wide range of human cancers.…”

mentioning

confidence: 99%

Downregulation of miR-21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status

Zhou

Ren

Moore

et al. 2010

Laboratory Investigation

321

247

View full text Add to dashboard Cite

MicroRNAs (miRNAs) are a class of endogenous small noncoding RNAs that regulate gene expression after transcription. Aberrant expression of miRNAs has been shown to be involved in tumorigenesis. We showed that miR-21 was one of the most frequently overexpressed miRNA in human glioblastoma (GBM) cell lines. To explore whether miR-21 can serve as a therapeutic target for glioblastoma, we downregulated miR-21 with a specific antisense oligonucleotide and found that apoptosis was induced and cell-cycle progression was inhibited in vitro in U251 (PTEN mutant) and LN229 (PTEN wildtype) GBM cells; xenograft tumors from antisense-treated U251 cells were suppressed in vivo. Antisense-miR-21-treated cells showed a decreased expression of EGFR, activated Akt, cyclin D, and Bcl-2. Although miR-21 is known to regulate PTEN and downregulation of miR-21 led to increased PTEN expression both endogenously and in a reporter gene assay, the GBM suppressor effect of antisense-miR-21 is most likely independent of PTEN regulation because U251 has mutant PTEN. Microarray analysis showed that the knockdown of miR-21 significantly altered expression of 169 genes involved in nine cell-cycle and signaling pathways. Taken together, our studies provide evidence that miR-21 may serve as a novel therapeutic target for malignant gliomas independent of PTEN status. Malignant gliomas are the most common primary brain tumors with high mortality and morbidity. The prognosis for malignant gliomas has not significantly improved in the last four decades. A recent meta-analysis of 12 randomized clinical trials showed that the overall survival rate of highgrade gliomas was 40% at 1 year after surgical removal and only slightly higher, 46%, after combined radiotherapy and chemotherapy. 1 To develop more optimized and effective treatment strategies for malignant gliomas, it is critical to gain deeper understanding of the molecular mechanisms underlying gliomagenesis and to identify targets for therapeutic intervention.The microRNAs (miRNAs) are a class of highly conserved small non-coding RNAs, approximately 22 nucleotides in length, that control gene expression through binding to the seed sequence at the 3 0 -UTR (untranslated region) of target mRNAs, resulting in translational repression or mRNA degradation. 2 This regulatory mechanism was first shown in the developmental processes in worms, flies, and plants. [3][4][5] Subsequently, miRNAs have been shown to have important roles in many physiological processes of mammalian systems by influencing cell apoptosis, proliferation, differentiation, development, and metabolism through regulation of critical signaling molecules including cytokines, growth factors, transcription factors, and pro-apoptotic and anti-apoptotic proteins. [6][7][8] Increasing number of miRNAs have been identified in the human genome and they are collectively called the miRNome. 9 Accumulating evidence shows the potential

show abstract

Expression profile of microRNAs in c-Myc induced mouse mammary tumors

Cited by 74 publications

References 64 publications

Mantle cell lymphoma: transcriptional regulation by microRNAs

Mantle cell lymphoma: transcriptional regulation by microRNAs

Noncoding RNAs in breast cancer

Downregulation of miR-21 inhibits EGFR pathway and suppresses the growth of human glioblastoma cells independent of PTEN status

Contact Info

Product

Resources

About