Expression profile of lipoxygenases in gingival tissues of human periodontitis

Abstract

Objectives This study aimed to clarify the expression profile and significance of lipoxygenases in periodontitis. Materials and Methods The mRNA levels of lipoxygenases in gingival tissues from 14 patients with periodontitis and 14 healthy individuals were determined by real‐time PCR, and validated in datasets, http://GSE16134 and http://GSE10334, and by Western blotting. Correlation of differentially expressed lipoxygenases with clinical parameters and expression of tumor necrosis factor‐α (TNF‐α), interleukin‐1β, matrix metalloproteinase (MMP)‐8, MMP‐9, and receptor activator of nuclear factor‐κB ligand (RANKL) was investigated in patients with periodontitis by Spearman's correlation analysis. Results The expression of ALOX5 (2.1‐fold, p < .05), ALOX12B (2.9‐fold, p < .001), and ALOX15B (9.4‐fold, p < .001) was upregulated in gingival tissues from patients with periodontitis, which was validated by dataset analysis and Western blotting. Positive correlations were observed between ALOX5 and probing depth, and ALOX15B and probing depth and clinical attachment loss. Furthermore, ALOX5 expression was positively correlated with TNF‐α, MMP‐8, MMP‐9, and RANKL expression, and ALOX15B was positively correlated with MMP‐8 and RANKL. Conclusions Our findings indicated the upregulation of ALOX5 and ALOX15B in periodontitis and suggested that ALOX5 and ALOX15B may be involved in periodontitis pathogenesis, including inflammation, connective tissue destruction, and abnormal bone metabolism.

Assessment of Periodontitis in Removable Partial Denture Wearers in District Buner

“…Periodontitis is initiated and perpetuated by a small group of predominantly gram-negative, anaerobic or microaerophilic bacteria that colonize the subgingival area. Indeed, at the 1996 World Workshop on Clinical Periodontics, the relevant working group concluded that most human periodontitis is caused by Porphyrornonas gingivalis, Bacteroides forsythus and Actinobacillus actinornyceterncornitans 11 . RPD design plays an important role in the state of the periodontium.…”

Identification of inflammation-related DNA methylation biomarkers in periodontitis patients based on weighted co-expression analysis

“…The methylation data came from the GPL13534 platform (Illumina HumanMethylation450 BeadChip). The gene expression profile data was from GPL570 platform (Affymetrix Human Genome U133 Plus 2.0 Array) numbered GSE10334 [44]. The data set contained a total of 183 periodontitis patient samples and 64 healthy control samples.…”

Identification of potentially functional circRNAs and prediction of circRNA‐miRNA‐mRNA regulatory network in periodontitis: Bridging the gap between bioinformatics and clinical needs

“…In addition to an initial factor of periodontal biofilms, genetic and epigenetic factors were identified to affect periodontitis progression 3,4 . Apart from well‐revealed typical expression profiles of genes in diseased periodontal tissues, epigenetic factors, especially noncoding RNAs (ncRNAs) have been recently described as important players involved in periodontitis pathogenesis 4–6 …”

“…3,4 Apart from well-revealed typical expression profiles of genes in diseased periodontal tissues, epigenetic factors, especially noncoding RNAs (ncRNAs) have been recently described as important players involved in periodontitis pathogenesis. [4][5][6] Among the noncoding RNAs, circular RNAs (circRNAs) are formed from the covalent linkage of the end of a single RNA molecule due to a back-splicing variant of expression. 7 Through the formation of the covalently closed-loop structure, the stability of circRNA could be substantially improved.…”