Expression profile of immune-related genes in Lates calcarifer infected by Cryptocaryon irritans

Mohd-Shaharuddin, Norashikin; Mohd-Adnan, Adura; Kua, Beng Chu; Nathan, Sheila

doi:10.1016/j.fsi.2012.11.052

Cited by 47 publications

(16 citation statements)

References 49 publications

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“…To date, no SAA-deficient animals have been generated, suggesting that it may play a vital role in the survival of multicellular organisms (Eklund et al, 2012). Our results support earlier findings of the increases SAA gene expression in the liver and kidney of common carp infected with the Trypanoplasma borreli (Saeij et al, 2003), and Asian sea bass infected with Cryptocaryon irritans (Mohd-Shaharuddin et al, 2013). In goldfish spleen, SAA was highly expressed throughout the course of infection.…”

Section: Discussionsupporting

confidence: 90%

“…This is similar to findings reported for Atlantic salmon infected with A. salmonicida, where the SAA expression was significantly elevated on 13 dpi (Vanya Ewart et al, 2005). In contrast, SAA was down-regulated on 10 dpi in the spleen of Asian sea bass infected with C. irritans (Mohd-Shaharuddin et al, 2013). There are also reports that SAA expression was highly variable upon parasite challenge (Tadiso et al, 2011), suggesting that changes in SAA expression in host tissues may be pathogen-dependent.…”

Section: Discussionsupporting

confidence: 86%

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The analysis of the acute phase response during the course of Trypanosoma carassii infection in the goldfish ( Carassius auratus L.)

Hagen

Xie

Belosevic

2015

Developmental & Comparative Immunology

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 86%

The analysis of the acute phase response during the course of Trypanosoma carassii infection in the goldfish ( Carassius auratus L.)

Hagen

Xie

Belosevic

2015

Developmental & Comparative Immunology

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“…The quality of RNA was checked by gel electrophoresis and, the purity and quantity were determined gel electrophoresis before synthesizing complementary DNA (cDNA) from 1 μg of total RNA using Omnicript RT kit (Qiagen, Hilden, Germany) following the instruction of manufacturer's company. qRT-PCR on stress-related genes were performed by PowerUp TM Cyber Green Master Mix (Thermo Scientific, USA) with 7500 Real-Time PCR System (Applied Biosystems, USA) and data were normalised against housekeeping genes, 18S rRNA and Ef1-a, (Table 4) and analysed using REST © software [48].…”

Section: Rna Extraction and Qrt-pcr Analysismentioning

confidence: 99%

Total replacement of fishmeal with poultry by-product meal affected the growth, muscle quality, histological structure, antioxidant capacity and immune response of juvenile barramundi, Lates calcarifer

2020

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The present study investigates if the total replacement of dietary fishmeal (FM) with poultry by-product meal (PBM), supplemented with methionine influences the muscle fatty acids composition, normal gut morphology, histological traits of the liver, muscle, and gill, liver enzymes, immune and antioxidant response, and stress-related gene in juvenile barramundi, Lates calcarifer in relation to growth and feed utilization. Barramundi (3.58±0.01g) were randomly distributed into six 300 L seawater recirculating tanks (25 fish/tank) and fed two formulated isonitrogenous and isolipidic diets for 6 weeks. The control diet had FM as the sole animal protein source, whereas other test diet had only PBM as an animal protein source. Dietary PBM affected the fish performance and feed utilization. Regarding muscle fatty acid profile, total saturated fatty acids and monounsaturated fatty acids elevated while total PUFA particularly n-3 LC-PUFA and EPA decreased in PBM fed fish than control diet fed fish. Liver, muscle, gill, and intestinal histology showed no obvious alteration in control diet fed fish, however, more lipid droplets and hepatic vacuolization in the liver, necrotic myotome in muscle, hyperplasia in secondary lamellae in gill and short and broken folds in the intestine were observed in PBM fed fish. Similar to light microscopy observation of intestinal morphology, the transmission electron microscopy (TEM) analysis revealed shorter and smaller microvilli in fish fed PBM. Histopathological alterations in the liver of PBM fed fish were further associated with the elevated levels of aspartate aminotransferase (AST) and glutamate dehydrogenase (GLDH) and the significant upregulation of stress-related genes, HSP70 and HSP90. Also, a negative influence on lysozyme activity, and antioxidant enzymatic activities were recorded in fish fed PBM. Overall, it can be concluded that a total substitution of FM protein by methionine supplemented PBM negatively influenced the growth performance, liver health, histological traits of different organs, immune and antioxidant response, and expression of stress-related genes in juvenile barramundi.

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“…Fish hepcidins have been shown to respond to several infectious stimuli, such as Gram-negative and Gram-positive bacteria (7,16,20,21,(23)(24)(25)(26)(27)(28)(29), virus (16,24), parasites (30), and LPS (17,18,22,(31)(32)(33), in some cases with different isoforms presenting differential expressions (16-18, 24, 29, 33). Other studies have examined the effects of iron overload in hepcidin (16-18, 21, 24, 28, 32), showing its involvement in the control of iron levels in fish.…”

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confidence: 99%

Multiple Hepcidins in a Teleost Fish, Dicentrarchus labrax: Different Hepcidins for Different Roles

Neves

Caldas

Vieira

et al. 2015

The Journal of Immunology

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Teleost fish rely heavily on their innate immunity for an adequate response against pathogens and environmental challenges, with the production of antimicrobial peptides being one of their first lines of defense. Among those is hepcidin, a small cysteine-rich antimicrobial peptide that is also the key regulator of iron metabolism. Although most mammals possess a single hepcidin gene, with a dual role in both iron metabolism regulation and antimicrobial response, many teleost fish present multiple copies of hepcidin, most likely because of genome duplications and positive Darwinian selection, suggesting that different hepcidins may perform different functions. To study the roles of hepcidin in teleost fish, we have isolated and characterized several genes in the European sea bass (Dicentrarchus labrax) and evaluated variations in their expression levels in response to different experimental conditions. Although several hepcidin genes were found, after phylogenetic analysis they could be clustered in two groups: hamp1-like, with a single isoform similar to mammalian hepcidins, and hamp2-like, with several isoforms. Under experimental conditions, hamp1 was upregulated in response to iron overload and infection and downregulated during anemia and hypoxic conditions. Hamp2 did not respond to either iron overload or anemia but was highly upregulated during infection and hypoxia. In addition, Hamp2 synthetic peptides exhibited a clear antimicrobial activity against several bacterial strains in vitro. In conclusion, teleost fish that present two hepcidin types show a degree of subfunctionalization of its functions, with hamp1 more involved in the regulation of iron metabolism and hamp2 mostly performing an antimicrobial role.

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Expression profile of immune-related genes in Lates calcarifer infected by Cryptocaryon irritans

Cited by 47 publications

References 49 publications

The analysis of the acute phase response during the course of Trypanosoma carassii infection in the goldfish ( Carassius auratus L.)

The analysis of the acute phase response during the course of Trypanosoma carassii infection in the goldfish ( Carassius auratus L.)

Total replacement of fishmeal with poultry by-product meal affected the growth, muscle quality, histological structure, antioxidant capacity and immune response of juvenile barramundi, Lates calcarifer

Multiple Hepcidins in a Teleost Fish, Dicentrarchus labrax: Different Hepcidins for Different Roles

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