Expression Profile of Carotenoid Cleavage Dioxygenase Genes in Summer Squash (Cucurbita pepo L.)

González-Verdejo, Clara Isabel; Obrero, Ángeles; Román, Belén; Gómez, Pedro Sánchez

doi:10.1007/s11130-015-0482-9

Cited by 22 publications

(14 citation statements)

References 28 publications

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“…This is in contrast to some cultivars of summer squash, where levels of carotenoids in the fruit were inversely correlated with CCD expression. 63 …”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin

et al. 2016

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Both the fruit mesocarp and the seeds of winter squash can be used for consumption, although the focus of breeding efforts varies by cultivar. Cultivars bred for fruit consumption are selected for fruit mesocarp quality traits such as carotenoid content, percent dry matter, and percent soluble solids, while these traits are essentially ignored in oilseed pumpkins. To compare fruit development in these two types of squash, we sequenced the fruit transcriptome of two cultivars bred for different purposes: an acorn squash, ‘Sweet REBA’, and an oilseed pumpkin, ‘Lady Godiva’. Putative metabolic pathways were developed for carotenoid, starch, and sucrose synthesis in winter squash fruit and squash homologs were identified for each of the structural genes in the pathways. Gene expression, especially of known rate-limiting and branch point genes, corresponded with metabolite accumulation both across development and between the two cultivars. Thus, developmental regulation of metabolite genes is an important factor in winter squash fruit quality.

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“…This is in contrast to some cultivars of summer squash, where levels of carotenoids in the fruit were inversely correlated with CCD expression. 63 …”

Section: Discussionmentioning

confidence: 99%

Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin

et al. 2016

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“…Furthermore, plant–insect interactions, extreme temperatures, high irradiance, or ultraviolet (UV) stress induce the production of β-ionone and β-cyclocitral among other apocarotenoid volatiles [108,109]. CCD1 expression has been detected in all tested tissues in different plant species [89,90,91,95,98,110,111], but its expression is stimulated by abiotic stress or ABA treatment [112,113]. …”

Section: Plant Ccdsmentioning

confidence: 99%

“…In grape, CCD4a and b genes are expressed in the berry, but only CCD4b shows a fruit-specific expression profile, highly upregulated during ripening, linking its activity to nor-isoprenoid volatiles production [98]. In summer squash ( Cucurbita pepo ) varieties with white, green, and yellow-orange coloration, the expression of two CCD4 genes ( a , b ) correlates inversely with carotenoid accumulation in the peel and flesh; therefore, both genes are good candidates for regulating fruit color [111,157]. One of the best-characterized examples of the CCD4 role in fruit pigmentation has been described in peach ( Prunus persica ).…”

Section: Plant Ccdsmentioning

confidence: 99%

Carotenoid Cleavage Oxygenases from Microbes and Photosynthetic Organisms: Features and Functions

Ahrazem

Gómez-Gómez

Rodrigo

et al. 2016

IJMS

137

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Apocarotenoids are carotenoid-derived compounds widespread in all major taxonomic groups, where they play important roles in different physiological processes. In addition, apocarotenoids include compounds with high economic value in food and cosmetics industries. Apocarotenoid biosynthesis starts with the action of carotenoid cleavage dioxygenases (CCDs), a family of non-heme iron enzymes that catalyze the oxidative cleavage of carbon–carbon double bonds in carotenoid backbones through a similar molecular mechanism, generating aldehyde or ketone groups in the cleaving ends. From the identification of the first CCD enzyme in plants, an increasing number of CCDs have been identified in many other species, including microorganisms, proving to be a ubiquitously distributed and evolutionarily conserved enzymatic family. This review focuses on CCDs from plants, algae, fungi, and bacteria, describing recent progress in their functions and regulatory mechanisms in relation to the different roles played by the apocarotenoids in these organisms.

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“…Terpenoids and their derivatives have attracted great interest for their commercial application as biofuels, flavoring ingredients, fragrances, antiseptics and pharmaceuticals [1][2][3][4]. β-ionone, an apocarotenoid derived from carotenoids (C40 terpenoids), has a warm, woody, and violet-like aroma, a low odor threshold, and has been widely used in the food and cosmetic industries [5]. Moreover, β-ionone is a key intermediate for the synthesis of vitamins A, E and K [6].…”

Section: Introductionmentioning

confidence: 99%

A modular pathway engineering strategy for the high-level production of β-ionone in Yarrowia lipolytica

Yang

Lin

et al. 2020

Microb Cell Fact

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Background: The GRAS and oleaginous yeast Yarrowia lipolytica (Y. lipolytica) is an attractive cell factory for the production of chemicals and biofuels. The production of many natural products of commercial interest have been investigated in this cell factory by introducing heterologous biosynthetic pathways and by modifying the endogenous pathways. However, since natural products anabolism involves long pathways and complex regulation, re-channelling carbon into the product of target compounds is still a cumbersome work, and often resulting in low production performance. Results: In this work, the carotenogenic genes contained carB and bi-functional carRP from Mucor circinelloides and carotenoid cleavage dioxygenase 1 (CCD1) from Petunia hybrida were introduced to Y. lipolytica and led to the low production of β-ionone of 3.5 mg/L. To further improve the β-ionone synthesis, we implemented a modular engineering strategy for the construction and optimization of a biosynthetic pathway for the overproduction of β-ionone in Y. lipolytica. The strategy involved the enhancement of the cytosolic acetyl-CoA supply and the increase of MVA pathway flux, yielding a β-ionone titer of 358 mg/L in shake-flask fermentation and approximately 1 g/L (~ 280-fold higher than the baseline strain) in fed-batch fermentation. Conclusions: An efficient β-ionone producing GRAS Y. lipolytica platform was constructed by combining integrated overexpressed of heterologous and native genes. A modular engineering strategy involved the optimization pathway and fermentation condition was investigated in the engineered strain and the highest β-ionone titer reported to date by a cell factory was achieved. This effective strategy can be adapted to enhance the biosynthesis of other terpenoids in Y. lipolytica.

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Expression Profile of Carotenoid Cleavage Dioxygenase Genes in Summer Squash (Cucurbita pepo L.)

Cited by 22 publications

References 28 publications

Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin

Comparative analysis of Cucurbita pepo metabolism throughout fruit development in acorn squash and oilseed pumpkin

Carotenoid Cleavage Oxygenases from Microbes and Photosynthetic Organisms: Features and Functions

A modular pathway engineering strategy for the high-level production of β-ionone in Yarrowia lipolytica

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