Expression profile and thyroid hormone responsiveness of transporters and deiodinases in early embryonic chicken brain development

Herck, Stijn L.J. Van; Geysens, Stijn; Delbaere, Joke; Tylzanowski, Przemko; Darras, Veerle

doi:10.1016/j.mce.2011.11.012

Cited by 44 publications

(55 citation statements)

References 43 publications

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“…High magnification imaging revealed EGFP expression in various cell types, including epithelial cells above the otic vesicle, in the olfactory bulb, and in the choroid plexus. This expression pattern is consistent with previous reports on chicken embryos (42,49) and mammals (43). The expression pattern in the choroid plexus suggests that MCT8 may play a role in the transport of THs into the cerebrospinal fluid.…”

Section: Discussionsupporting

confidence: 92%

Zebrafish as a Model for Monocarboxyl Transporter 8-Deficiency

Vatine

Zada

Lerer-Goldshtein

et al. 2013

Journal of Biological Chemistry

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Background: Mutations in the thyroid hormone transporter MCT8 are associated with the psychomotor retardation Allan-Herndon-Dudley syndrome (AHDS). Results: In zebrafish, as in humans, mct8 is expressed primarily in the nervous system. Elimination of MCT8 causes severe neural impairment. Conclusion: MCT8 is a crucial regulator during zebrafish embryonic development. Significance: Establishment of the first vertebrate model for MCT8 deficiency, which exhibits a neurological phenotype.

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Section: Discussionsupporting

confidence: 92%

Zebrafish as a Model for Monocarboxyl Transporter 8-Deficiency

Vatine

Zada

Lerer-Goldshtein

et al. 2013

Journal of Biological Chemistry

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“…There were no other reports of Dio1 and Dio2 expression in early embryonic stage for fish, but some data were available in amphibians, birds and mammals to support the presence of deiodinase expression in embryos. In most cases, significant Dio1 Dio2 and Dio3 expression was found in the developing brain, which is highly sensitive to thyroid hormones (Horn and Heuer, 2010; Johnson and Lema, 2011; Kaplan and Yaskoski, 1981; Tu et al, 1999; Van Herck et al, 2012). Dio1 and Dio2 are known to play an important role in fish embryonic development (Eales et al, 1993; Kohrle, 2000).…”

Section: Discussionmentioning

confidence: 99%

Using whole mount in situ hybridization to examine thyroid hormone deiodinase expression in embryonic and larval zebrafish: A tool for examining OH-BDE toxicity to early life stages

et al. 2013

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Polybrominated diphenyl ethers (PBDEs) and their oxidative metabolites (hydroxylated PBDEs; OH-BDEs) are known endocrine disrupting contaminants that have been shown to disrupt thyroid hormone regulation both in mammals and in fish. The purpose of this study was to determine the precise organ and tissue locations that express genes critical to thyroid hormone regulation in developing zebrafish (Danio rerio), and to determine the effects of an OH-BDE on their expression. While RT-PCR can provide quantitative data on gene expression, it lacks spatial sensitivity to examine localized gene expression; and, isolation of organs from zebrafish embryos is technically difficult, if not impossible. For this reason, the present study used whole mount in situ hybridization to simultaneously localize and quantify gene expression in vivo. While PBDEs and OH-BDEs have been shown to inhibit the activity and expression of deiodionases, a family of enzymes that regulate thyroid hormone concentrations intracellularly, it is unclear whether or not they can affect regional expression of the different isoforms during early development. In this study we investigated deiodinase 1 (Dio1), deiodinase 2 (Dio2), and deiodinase 3 (Dio3) mRNA expression at the following life stages (2, 8, and 1k-cells; 50%-epiboly, 6 and 18-somites, 22, 24, 48, 72 hpf and/or 10 dpf) in zebrafish and found life stage specific expression of these genes that were highly localized. To demonstrate the use of this technique for investigating potential endocrine disrupting effects, zebrafish embryos were exposed to 1, 10 and 100 nM 6-OH-BDE-47. Significant increases in mean intensity of Dio1 and Dio3 expression in the periventricular zone of brain and pronephric duct, respectively (quantified by measuring intensity of coloration using ImageJ analysis software) were observed, suggesting localized response at the HPT axis with the possibility of impacting neurodevelopment. Our results demonstrate effects of OH-BDEs on thyroid regulating gene expression and provide more insight into potential sites of injury during early life stages.

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“…about the developmental thyroid hormone mechanisms (deiodinases, transporters, sulfotransferases and receptors) in human [1,2,50,52,127,130,[151][152][153][154], rat [1,2,41,60,135,[154][155][156] and chicken [7,[157][158][159][160][161][162][163][164][165][166][167][168][169][170]. Note that the chicken is born early compared to the rat and human, as well as the rat is born early compared to the human (Table 6).…”

Section: Discussionmentioning

confidence: 99%

“…On account of TH action can be controlled in individual cells through selective TH uptake and intracellular TH metabolism, the placenta is an important link in the maternal-fetal communication network for THs which are essential for the normal development and differentiation of the fetus [1][2][3]. Generally, intracellular activation or inactivation of Lthyroxine (T4) and 3,5,3'-triiodothyronine (T3) in turn is determined by three types of iodothyronine deiodinases (Ds), namely DI, DII, and DIII [4][5][6][7]. The placenta transports and metabolizes maternal THs, and mainly expresses DIII, which inactivates T4 and other iodothyronines and thus limits the transfer of maternal active THs to the fetus in the late pregnancy [8].…”

Section: Introductionmentioning

confidence: 99%

Maternal-Fetal Thyroid Interactions

Ahmed¹

2012

Thyroid Hormone

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Expression profile and thyroid hormone responsiveness of transporters and deiodinases in early embryonic chicken brain development

Cited by 44 publications

References 43 publications

Zebrafish as a Model for Monocarboxyl Transporter 8-Deficiency

Zebrafish as a Model for Monocarboxyl Transporter 8-Deficiency

Using whole mount in situ hybridization to examine thyroid hormone deiodinase expression in embryonic and larval zebrafish: A tool for examining OH-BDE toxicity to early life stages

Maternal-Fetal Thyroid Interactions

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