Expression profile and functional characterization of odorant binding proteins in a forest pest, Dioryctria abietella (Lepidoptera: Pyralidae)

Guo, Yu-Ruo; Yin, Ning-Na; Chun, Wang; Yang, Zixuan; Wang, Zhengquan; Liu, Nai-Yong

doi:10.1016/j.cbpb.2023.110835

Cited by 2 publications

(1 citation statement)

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“…Insects have a high precision and complex olfactory system to guide a series of behaviors such as feeding, mating, host plant location, and oviposition . A variety of chemosensory genes are involved in the olfactory system, including odorant binding proteins (OBPs), , chemosensory proteins (CSPs), , gustatory odorants (GRs), , odorant receptors (ORs), , ionotropic receptors (IRs), , odorant-degrading enzymes (ODEs), , and sensory neuron membrane proteins (SNMPs). , …”

Section: Introductionmentioning

confidence: 99%

Binding Properties of Odorant Binding Protein 37 in Plagiodera versicolora to Host Volatile, o-Cymene

Liu,

Pei,

et al. 2024

J. Agric. Food Chem.

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The chemosensory system plays an important role in the host plants location. Plagiodera versicolora (Coleoptera: Chrysomelidae) is a worldwide leaf-eating forest pest that feeds exclusively on salicaceous trees. There is no function study of odorant binding proteins (OBPs) in P. versicolora. In the current study, we found that PverOBP37 has a high expression in male and female antennae, heads, and legs by quantitative real-time PCR. The binding properties of PverOBP37 to 18 host plant volatiles were determined by fluorescence competition binding assays. The results showed that PverOBP37 could bind to the host plant volatile, o-cymene. Furthermore, four candidate key amino acid residues (F8, Y50, F103, and R107) of PverOBP37 to o-cymene were identified by molecular docking. The functional assay to confirm Y50, F103, and R107 mutations were key amino acid residues of PverOBP37 involved in the binding to o-cymene. Knockdown of PverOBP37 and Y-tube behavioral bioassays of mated females led to a significantly reduced attraction to o-cymene. This study not only revealed the molecular mechanism of PverOBP37 but also suggested that PverOBP37 is essential to detect host plant volatiles as cues to search for egg-laying sites in P. versicolora.

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