Expression Patterns of the Implantation-associated Genes in the Uterus during the Estrous Cycle in Mice

Lee, Dong‐Soo; Ueta, Yoshiko Yanagimoto; Xuan, Xuenan; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Toyoda, Yutaka; Suzuki, Hiroshi

doi:10.1262/jrd.17039

Cited by 20 publications

(17 citation statements)

References 57 publications

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“…GAPDH was used as a reference control to normalize the loading of template cDNA. The sequences of each pair of primers were as follows: HB-EGF forward: 59-TCCGTCTGTCTTCTTGTCATCGT-39, HB-EGF reverse: 59-TAGCCACGCCCAACTTCACT-39; EGF forward: 59-CCAAACGCGAAGACTTATCC-39, EGF reverse: 59-TGATCCTCAAA-CACGGCTAGAGA-39; TGF-a forward: 59-GCCCAGATTCCCACACT CAGTA-39, TGF-a reverse: 59-TCTGCATGCTCACAGCGAA-39; EGFR forward: 59-CATCTGTGCCCAGCAATGTT-39, EGFR reverse: 59-TTGC-ATGTGGCCTCATCTTG-39; and GAPDH forward: 59-TGCATCCTGCA-CCACCAACTGCTTAG-39, GAPDH reverse: 59-GGCATGGACTGTGGT-CATGA-39 (20,21). …”

Section: Quantitative Real-time Pcrmentioning

confidence: 99%

The Overexpression of Heparin-Binding Epidermal Growth Factor Is Responsible for Th17-Induced Airway Remodeling in an Experimental Asthma Model

Wang¹,

Li²,

Yao³

et al. 2010

The Journal of Immunology

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Section: Quantitative Real-time Pcrmentioning

confidence: 99%

The Overexpression of Heparin-Binding Epidermal Growth Factor Is Responsible for Th17-Induced Airway Remodeling in an Experimental Asthma Model

Wang¹,

Li²,

Yao³

et al. 2010

The Journal of Immunology

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“…On the other hand, it has been shown that amphiregulin mRNA is expressed throughout the e s t r o u s c y c l e i n t h e m o u s e u t e r u s [ 1 4 ] . Furthermore, the highest expression level of amphiregulin during diestrus is not consistent with the LIF mRNA expression pattern during the estrous cycle [14]. As shown in Fig.…”

Section: Discussionmentioning

confidence: 67%

“…The gene expression rate was obtained by normalizing the amount of amphiregulin cDNA with that of beta-actin. Total RNA from the oviducts and uteri of nonpregnant a n i m a l s w e r e u s e d a s c o n t r o l s b e c a u s e amphiregulin mRNA is stably expressed during the estrous cycle [14].…”

Section: Real-time Quantitative Pcrmentioning

confidence: 99%

Expression of Amphiregulin During the Pre- and Post-implantation Period in the Mouse Reproductive Tract

2006

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Abstract. In mammals, embryo implantation is an essential step in reproduction. Implantation is a phenomenon that involves crosstalk between the blastocyst and the maternal endometrium. However, the molecular basis of the connections between the blastocyst and endometrium is not yet fully understood. Amphiregulin is a member of the epidermal growth factor family and is known to be expressed in the luminal epithelium of the mouse uterus on 3.5 days post coitum (dpc). Thus, to clarify the mechanism of amphiregulin at fetomaternal interface, we analyzed the expression pattern of amphiregulin mRNA in the oviducts and uteri of pregnant and psuedopregnant mice by means of real-time PCR. Amphiregulin expression in the pregnant uterus dramatically increased on 2.5 dpc, peaked on 3.5 dpc, and declined by 5.5 dpc. Furthermore, to analyze the effect of the presence of an embryo on amphiregulin expression, we determined the expression pattern of amphiregulin mRNA in the uterus after embryo transfer on 0.5 and 1.5 dpc. A previous study showed that the expression of amphiregulin mRNA depends on the concentration of progesterone. However, our present results indicate that amphiregulin mRNA is upregulated by the presence of fertilized eggs in the lumen of the oviduct on 0.5 dpc.

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“…The homology of rat USAG-1 cDNA to those of mouse and human is 96% and 91%, respectively [7]. W e p r e v i o u s l y i d e n t i f i e d U S A G -1 m R N A expression in mouse uteri during the estrous cycle [8]. In contrast to the temporally regulated expressions of COX-1, COX-2, EGF, LIF, and other growth factors/cytokines, USAG-1 mRNA expression did not change during the estrous cycle [8].…”

mentioning

confidence: 99%

“…W e p r e v i o u s l y i d e n t i f i e d U S A G -1 m R N A expression in mouse uteri during the estrous cycle [8]. In contrast to the temporally regulated expressions of COX-1, COX-2, EGF, LIF, and other growth factors/cytokines, USAG-1 mRNA expression did not change during the estrous cycle [8]. However, the expression pattern and function of USAG-1 in the mouse uterus during the periimplantation period have not been studied.…”

mentioning

confidence: 99%

Expression of Uterine Sensitization-Associated Gene-1 (USAG-1) in the Mouse Uterus During the Peri-Implantation Period

Maeda

Lee

Ueta

et al. 2007

Journal of Reproduction and Development

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Abstract. Rat uterine sensitization-associated gene-1 (USAG-1) mRNA is expressed in the uterus during the peri-implantation period, and its mRNA expression in uterine epithelial cells is highest on day 5 of pregnancy. On the other hand, since changes in USAG-1 mRNA expression in the mouse uterus are not seen during the estrous cycle, USAG-1 expression might be specifically regulated by embryonic factors rather than by the maternal environment. However, the expression pattern and function of USAG-1 in the mouse uterus have not been determined. Thus, we examined the tissuespecific USAG-1 mRNA expression in the uteri of ICR mice during peri-implantation using real-time quantitative PCR. Uterine tissues, such as the myometrium, luminal epithelium, and stroma, were collected by laser capture microdissection at 3.5-6.5 dpc. USAG-1 mRNA was expressed in the uteri of pregnant mice from 3.5 dpc to 6.5 dpc, and the highest level of expression was seen at 4.5 dpc (P<0.01). Significantly high USAG-1 mRNA expression was detected in the luminal epithelium at 4.5 dpc (P<0.05). The stroma and myometrium exhibited unchanged expression levels of USAG-1 mRNA at 3.5-5.5 dpc. USAG-1 mRNA was undetectable in blastocysts and implanting embryos. Expression of USAG-1 mRNA appears to be associated with blastocyst implantation to the luminal epithelium, suggesting that physiological or biochemical contact of the blastocyst to the uterus is required for USAG-1 expression. Key words: Implantation, Mouse, mRNA, Uterine sensitization-associated gene-1 (USAG-1), Uterus (J. Reprod. Dev. 53: [931][932][933][934][935][936] 2007) or successful establishment of pregnancy, blastocyst development must proceed beyond the attachment stage and result in endometrial decidualization and subsequent placental formation. Estrogen and progesterone regulate the events that lead to the receptivity and sensitization of the endometrium for the decidual cell reaction [1]. In rodents and humans, implantation is i n i t i a t e d w i t h a n a p p o s i t i o n b e t w e e n t h e trophoblast and apical surface of the luminal epithelium. This is followed rapidly by adhesion of the conceptus to the luminal surface and then penetration through the luminal epithelium to the underlying stroma, which initiates the endometrial d e c i d u a l i z a t i o n r e a c t i o n [ 2 , 3 ] . U t e r i n e sensitization-associated gene-1 (USAG-1), a gene of unknown function during conceptus development, is upregulated in the sensitized endometrium of the rat uterus during the peri-implantation period [4].

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Expression Patterns of the Implantation-associated Genes in the Uterus during the Estrous Cycle in Mice

Cited by 20 publications

References 57 publications

The Overexpression of Heparin-Binding Epidermal Growth Factor Is Responsible for Th17-Induced Airway Remodeling in an Experimental Asthma Model

The Overexpression of Heparin-Binding Epidermal Growth Factor Is Responsible for Th17-Induced Airway Remodeling in an Experimental Asthma Model

Expression of Amphiregulin During the Pre- and Post-implantation Period in the Mouse Reproductive Tract

Expression of Uterine Sensitization-Associated Gene-1 (USAG-1) in the Mouse Uterus During the Peri-Implantation Period

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