Expression patterns of signaling lymphocytic activation molecule family members in peripheral blood mononuclear cell subsets in patients with systemic lupus erythematosus

Abstract

Genome-wide linkage analysis studies (GWAS) studies in systemic lupus erythematosus (SLE) identified the 1q23 region on human chromosome 1, containing the Signaling Lymphocytic Activation Molecule Family (SLAMF) cluster of genes, as a lupus susceptibility locus. The SLAMF molecules (SLAMF1-7) are immunoregulatory receptors expressed predominantly on hematopoietic cells. Activation of cells of the adaptive immune system is aberrant in SLE and dysregulated expression of certain SLAMF molecules has been reported. We examined the expression of SLAMF1-7 on peripheral blood T cells, B cells, monocytes, and their respective differentiated subsets, in patients with SLE and healthy controls in a systematic manner. SLAMF1 levels were increased on both T cell and B cells and their differentiated subpopulations in patients with SLE. SLAMF2 was increased on SLE CD4+ and CD8+ T cells. The frequency of SLAMF4+ and SLAMF7+ central memory and effector memory CD8+ T cells was reduced in SLE patients. Naïve CD4+ and CD8+ SLE T cells showed a slight increase in SLAMF3 levels. No differences were seen in the expression of SLAMF5 and SLAMF6 among SLE patients and healthy controls. Overall, the expression of various SLAMF receptors is dysregulated in SLE and may contribute to the immunopathogenesis of the disease.

Identifying the pattern of immune related cells and genes in the peripheral blood of ischemic stroke

“…Costimulatory molecule and microbial sensor SLAMF1 (also known as CD150) initiates signal transduction pathways in a variety of immune cells [ 24 ]. SLAMF1 is overexpressed on the T cell, B cell and their subgroups of Systemic lupus erythematosus (SLE) patients [ 25 , 26 ]. Expression of SLAMF1 was significantly increased in peripheral blood CD4+, T helper 17, and CD19+ B cells from autoimmune thyroid disease patients [ 27 ].…”

Signaling Lymphocytic Activation Molecule Family Member 1 Engagement Inhibits T Cell–B Cell Interaction and Diminishes Interleukin‐6 Production and Plasmablast Differentiation in Systemic Lupus Erythematosus

“…B cells are potent antigen presenting cells, able to initiate and maintain T cell responses, both through cell-cell contact and cytokine release. SLAMF1 is upregulated on both T cells and B cells of patients with SLE (12). We examined the effect of SLAMF1 ligation using a specific anti-human SLAMF1 mAb in the context of T-B interactions in vitro .…”

“…The −262 A/G and −188 A/G polymorphisms in the promoter region of SLAMF1 are linked to higher SLAMF1 mRNA expression following stimulation of SLE peripheral blood mononuclear cells with phytohemagglutinin and may contribute to increased SLE susceptibility (10). Moreover, SLAMF1 is up-regulated on the cell surface of both T cells and B cells in the peripheral blood of patients with SLE, suggesting a potential role of SLAMF1 in SLE immunopathogenesis (11, 12).…”

SLE-Associated Defects Promote Altered T Cell Function

“…Several studies have examined the expression of SLAMF1 to 7 receptors on the surface of T cells isolated from the peripheral blood of SLE patients in comparison to healthy controls, and delivered variable results, thus reflecting the heterogeneity of the disease. 120–127 Two studies systematically examined the expression of SLAMF1 to 7 receptors on the T cells from cohorts of patients suffering from SLE. 120,121 In one, authors examined the expression of SLAMF receptors on T cells isolated from patients with lupus nephritis and showed that expression of SLAMF3, SLAMF5, and SLAMF7, on CD8 + and DN T cells was lower in patients who are in remission as compared to patients with active nephritis.…”