Expression patterns of seven key genes, including β-catenin, Notch1, GATA6, CDX2, miR-34a, miR-181a and miR-93 in gastric cancer

Abstract

Gastric cancer (GC) is one of the most prevalent cancers and a major cause of cancer related mortality worldwide. Incidence of GC is affected by various factors, including genetic and environmental factors. Despite extensive research has been done for molecular characterization of GC, it remains largely unknown. Therefore, further studies specially conducted among various ethnicities in different geographic locations, are required to know the precise molecular mechanisms leading to tumorigenesis and progression of GC. The expression patterns of seven candidate genes, including β-catenin, Notch1, GATA6, CDX2, miR-34a, miR-181a, and miR-93 were determined in 24 paired GC tissues and corresponding non-cancerous tissues by quantitative Real-Time PCR. The association between the expression of these genes and clinicopathologic factors were also investigated. Our results demonstrated that overall mRNA levels of GATA6 were significantly decreased in the tumor samples in comparison with the non-cancerous tissues (median fold change (FC) = 0.3143; P = 0.0003). Overall miR-93 levels were significantly increased in the tumor samples relative to the non-cancerous gastric tissues (FC = 2.441; P = 0.0002). β-catenin mRNA expression showed a strong positive correlation with miR-34a (r = 0.5784; P = 0.0031), and miR-181a (r = 0.5652; P = 0.004) expression. miR-34a and miR-181a expression showed a significant positive correlation (r = 0.4862; P = 0.016). Moreover, lower expression of Notch1 was related to distant metastasis in GC patients with a borderline statistical significance (p = 0.0549). These data may advance our understanding of the molecular biology that drives GC as well as provide potential targets for defining novel therapeutic strategies for GC treatment. Gastric cancer (GC) is one of the most prevalent cancers and the second leading cause of cancer-associated deaths worldwide. GC is a multifactorial disease affected by various genetic and environmental factors, including Helicobacter pylori infection, lifestyle, socioeconomic factors, dietary behavior, and aging 1,2. This matter reflects an imperative need for conduction of studies among different geographic locations and ethnicities to detect

The Role and Interactions of Programmed Cell Death 4 and its Regulation by microRNA in Transformed Cells of the Gastrointestinal Tract

“…In accord with other finding ( 162 , 163 ), the study by Liang et al. ( 155 ) found that miRNA-93 was also a possible oncomir in gastric cancer cells.…”

“…In accord with other finding (162,163), the study by Liang et al (155) found that miRNA-93 was also a possible oncomir in gastric cancer cells. The investigators used a miRNA array to examine two gastric cancer tissues and found that miRNA-23b-3p, miRNA-17-5p and miRNA-93 inversely correlated with PDCD4 expression and were therefore candidate regulators of the tumour suppressor.…”

“…( 161 ) and disabled 2 (DAB2) ( 159 ). Similarly, this microRNA has been found to be upregulated in gastric cancer ( 162 , 163 ) and expression correlates with poor survival and metastatic disease ( 164 , 165 ), Transfection and overexpression of miRNA-93 in gastric cancer cell lines also promotes an increase in proliferation, migration, invasion and chemoresistance ( 166 , 167 ) and decreases the expression of the tumour suppressors AHNAK ( 167 , 168 ) and TIMP-2 ( 165 ). However, there is conflicting data regarding the expression and contribution of miRNA-93 in colorectal cancer, as expression has been found to be increased in CRC tissue ( 169 ), downregulated by chemotherapeutic drugs ( 170 ) and negatively regulates the expression of the tumour suppressor PTEN in SW620 cells ( 171 ), yet has also been found to be downregulated in CRC tissue ( 172 – 174 ), with suppression associated with metastatic disease and poor overall survival ( 175 ).…”

miR-34a mimic or pre-mir-34a, which is the better option for cancer therapy? KatoIII as a model to study miRNA action in human gastric cancer cells

“…microRNAs (miRNAs, miRs) are small non-coding regulatory RNA molecules that play important roles in various biological processes. One miRNA could target many mRNAs, and most mammalian mRNAs are conserved targets of miRNAs [ 5 – 7 ]. At least three RNA species, primary miRNA (pri-miRNA), precursor miRNA (pre-miRNA), and mature miRNA, are generated from miRNA genes during transcription and sequential processing steps by Drosha and Dicer ribonucleases.…”

Predicting metastasis in Gastric cancer patients: machine learning-based approaches

“…Gastric cancer (GC) is considered as third invasive malignant growth across the globe [1]. Incidence of GC may occure by genetic and environmental effects in developing countries [2]. Although the morbidity and mortality of GC have reduced over the past few decades in some nations, the malignancy has remained the fourth leading cause of cancer-related deaths over the world [3].…”