Expression patterns of plexins and neuropilins are consistent with cooperative and separate functions during neural development

Mauti, Olivier; Sadhu, Rejina; Gemayel, Joëlle; Gesemann, Matthias; Stoeckli, Esther T.

doi:10.1186/1471-213x-6-32

Cited by 49 publications

(24 citation statements)

References 69 publications

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“…PlexinA4 was also found in virtually all DRG neurons. The distribution of PlexinA4 protein in the spinal cord and DRGs is consistent with that of its mRNA (Bron et al, 2007; Haklai-Topper et al, 2010; Mauti et al, 2006; Perala et al, 2005; Suto et al, 2003). In developing chicks, PlexinA4 mRNA becomes visible by stage 22 in the ventral horn and in the dorsal commissural neurons (Bron et al, 2007; Mauti et al, 2006).…”

Section: Discussionsupporting

confidence: 69%

“…The distribution of PlexinA4 protein in the spinal cord and DRGs is consistent with that of its mRNA (Bron et al, 2007; Haklai-Topper et al, 2010; Mauti et al, 2006; Perala et al, 2005; Suto et al, 2003). In developing chicks, PlexinA4 mRNA becomes visible by stage 22 in the ventral horn and in the dorsal commissural neurons (Bron et al, 2007; Mauti et al, 2006). In mouse embryos, PlexinA4 mRNA is found in the spinal cord where it is highly expressed in the ventral horn with more moderate expression in the dorsal horn (Perala et al, 2005; Suto et al, 2003).…”

Section: Discussionsupporting

confidence: 69%

“…We found the distribution of PlexinA4 protein in the adult spinal cord to be comparable to that of the known distribution of its co-receptor Nrp1 at least during development. Nrp1 mRNA is highly expressed in the MNs of developing chicks and rats, and it is also present in rat DRG neurons (Mauti et al, 2006; Takahashi et al, 1999). In addition, Nrp1 protein has been detected in the corticospinal tract of mice (Mire et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

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PlexinA4 distribution in the adult rat spinal cord and dorsal root ganglia

Gutekunst

Stewart

Franz

et al. 2012

Journal of Chemical Neuroanatomy

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PlexinsA1–A4 participate in class 3 semaphorin signaling as co-receptors to neuropilin 1 and 2, PlexinA4 being the latest member of the PlexinA subfamily to be identified. Little is known about the cellular distribution of PlexinA4 in the spinal cord and dorsal root ganglion (DRG). Here, immunohistochemical studies using antibodies to PlexinA4 revealed immunolabeling in neurons in both dorsal and, to a greater extent, ventral horns of the spinal cord. Ventral horn PlexinA4 positive neurons exhibited morphology, size, and location consistent with both motor neurons and interneurons. Labeling was found in motor axons exiting through the ventral roots, and more widespread labeling was observed in ascending and descending white matter tracts. Within the DRG, immunostaining was observed in neuronal cell bodies as well as the central and peripheral processes of these cells. PlexinA4 is expressed in the peripheral nervous system where its expression is regulated upon nerve injury. This is the first detailed description of the cellular and subcellular distribution of PlexinA4 in the adult spinal cord and DRG, and it will set the basis for future studies on the potential role of PlexinA4 in regeneration and repair of the adult central and peripheral nervous system.

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Section: Discussionsupporting

confidence: 69%

Section: Discussionsupporting

confidence: 69%

Section: Discussionmentioning

confidence: 99%

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PlexinA4 distribution in the adult rat spinal cord and dorsal root ganglia

Gutekunst

Stewart

Franz

et al. 2012

Journal of Chemical Neuroanatomy

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“…In mouse, Neuropilin-2, a receptor component for Semaphorins, has been shown to expel commissural axons from the floor plate [23]. However, in chick Neuropilin-2 is not prominently expressed in dorsolateral commissural neurons when their axons are crossing the floor plate [52]. …”

Section: Resultsmentioning

confidence: 99%

RabGDI controls axonal midline crossing by regulating Robo1 surface expression

et al. 2012

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BackgroundAxons navigate to their future synaptic targets with the help of choice points, intermediate targets that express axon guidance cues. Once they reach a choice point, axons need to switch their response from attraction to repulsion in order to move on with the next stage of their journey. The mechanisms underlying the change in axonal responsiveness are poorly understood. Commissural axons become sensitive to the repulsive activity of Slits when they cross the ventral midline of the CNS. Responsiveness to Slits depends on surface expression of Robo receptors. In Drosophila, Commissureless (Comm) plays a crucial regulatory role in midline crossing by keeping Robo levels low on precommissural axons. Interestingly, to date no vertebrate homolog of comm has been identified. Robo3/Rig1 has been shown to control Slit sensitivity before the midline, but without affecting Robo1 surface expression.ResultsWe had identified RabGDI, a gene linked to human mental retardation and an essential component of the vesicle fusion machinery, in a screen for differentially expressed floor-plate genes. Downregulation of RabGDI by in ovo RNAi caused commissural axons to stall in the floor plate, phenocopying the effect observed after downregulation of Robo1. Conversely, premature expression of RabGDI prevented commissural axons from entering the floor plate. Furthermore, RabGDI triggered Robo1 surface expression in cultured commissural neurons. Taken together, our results identify RabGDI as a component of the switching mechanism that is required for commissural axons to change their response from attraction to repulsion at the intermediate target.ConclusionRabGDI takes over the functional role of fly Comm by regulating the surface expression of Robo1 on commissural axons in vertebrates. This in turn allows commissural axons to switch from attraction to repulsion at the midline of the spinal cord.

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“…In situ hybridization was performed as previously described (Mauti et al, 2006). Spinal cord patterning was assessed as previously described (Avilés and Stoeckli, 2016).…”

Section: In Situ Hybridization and Immunohistochemistrymentioning

confidence: 99%

Calsyntenin 1-mediated trafficking of axon guidance receptors regulates the switch in axonal responsiveness at a choice point

Alther¹,

Domanitskaya²,

Stoeckli³

2016

Journal of Cell Science

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Axon guidance at choice points depends on the precise regulation of guidance receptors on the growth cone surface. Upon arrival at the intermediate target or choice point, a switch from attraction to repulsion is required for the axon to move on. Dorsal commissural (dI1) axons crossing the ventral midline of the spinal cord in the floor plate represent a convenient model for the analysis of the molecular mechanism underlying the switch in axonal behavior. We identified in chick a role for calsyntenin 1 in the regulation of vesicular trafficking of guidance receptors in dI1 axons at choice points. In cooperation with RabGDI, calsyntenin 1 shuttles Rab11-positive vesicles containing Robo1 to the growth cone surface in a precisely regulated manner. By contrast, calsyntenin 1-mediated trafficking of frizzled 3, a guidance receptor in the Wnt pathway, is independent of RabGDI. Thus, tightly regulated insertion of guidance receptors, which is required for midline crossing and the subsequent turn into the longitudinal axis, is achieved by specific trafficking.

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Expression patterns of plexins and neuropilins are consistent with cooperative and separate functions during neural development

Cited by 49 publications

References 69 publications

PlexinA4 distribution in the adult rat spinal cord and dorsal root ganglia

PlexinA4 distribution in the adult rat spinal cord and dorsal root ganglia

RabGDI controls axonal midline crossing by regulating Robo1 surface expression

Calsyntenin 1-mediated trafficking of axon guidance receptors regulates the switch in axonal responsiveness at a choice point

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