Expression patterns of miR-146a and miR-146b in mastitis infected dairy cattle

Wang, Xing Ping; Luoreng, Zhuo Ma; Zan, Linsen; Raza, Sayed Haidar Abbas; Li, Feng; Li, Na; Shuan, Liu

doi:10.1016/j.mcp.2016.08.004

Cited by 36 publications

(27 citation statements)

References 15 publications

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“…For example, several studies reported that the expressions of bovine miR-223 in mammary gland with mastitis was up-regulated in comparison to that of healthy cows 18 , 37 , 38 , and have also been observed to regulate the mammary innate immune response by directly targeting HMGB1 39 . Furthermore, our previous study identified that the expression of bovine miR-146a was significantly increased in bovine mammary tissues from cases of mastitis caused by S. aureus , E. coli or mixed bacterial infection 11 , and supports the idea that bovine miR-146a regulates the secretion of inflammatory cytokines such as TNF-α, IL-6, and IL-8 in bMECs 13 . It is also noteworthy that bta-miR-375 is one of most down-regulated DIE-miRNAs (6.20 and 4.67-fold change in S. aureus and E. coli infected mammary tissue, respectively), suggesting that it participates in the regulation of immune responses and inflammation.…”

Section: Discussionsupporting

confidence: 72%

“…To investigate the regulatory function of DIE-miRNAs in dairy cattle, we predicted potential targets of these miRNAs. Among the putative targets, bta-miR-146a and bta-miR-214 were identified to control genes responsible for regulating inflammation in bovine mammary epithelial cells 11 - 13 . In the present study, the putative target genes of DIE-miRNAs were annotated as being involved in various classes of immune signalling pathways, TLR signalling pathway, TGF-β signalling pathway, cytokine-cytokine receptor interaction, MAPK signalling pathway, cell adhesion molecules, leukocyte trans endothelial migration, and chemokine signalling pathways.…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have reported that the expression levels of many miRNAs detected by qPCR exhibit obvious changes in response to bovine mastitis or inflammatory pathway activation in bMECs. For example, it has been reported that the expressions of miRNAs (miR-223, miR-146a, miR-146b, miR-15a-3p, miR-15a-5p) were upregulated, whereas three miRNAs (miR-16a, -31, and -181a) were downregulated in infected versus healthy mammary tissue 11 , 18 , 19 . Although some reports have examined miRNAs and mastitis in dairy cows, the current understanding of the molecular mechanisms of the relationship is far from comprehensive.…”

Section: Introductionmentioning

confidence: 99%

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Comparison of microRNA Profiles between Bovine Mammary Glands Infected with Staphylococcus aureus and Escherichia coli

et al. 2018

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MicroRNAs (miRNAs) play crucial roles in regulating innate and adaptive immunity in humans and animals. Infection with E. coli or S. aureus can cause inflammation of the mammary glands, which results in significant economic losses in dairy cattle. However, the regulatory mechanisms of miRNAs in response to E. coli or S. aureus infection in bovine mammary glands have not been thoroughly explored. To discover the differential expression of miRNA in bovine mammary gland challenged with E. coli or S. aureus, we performed miRNA sequencing on tissue samples. A total of 1838 miRNAs were identified, including 580 known-miRNAs (included in the miRbase database) and 1258 predicted novel miRNAs. The miRNA expression patterns indicated that, compared with control samples, 279 miRNAs and 305 miRNAs were differentially expressed miRNAs (DIE-miRNA) in S. aureus and E. coli infected tissues, respectively. Moreover, the results of comparison the DIE-miRNAs between the E. coli and S. aureus infected groups showed that 197 DIE-miRNAs are identical, 108 DIE-miRNAs are specific to the E. coli group, and 82 DIE-miRNAs are specific to the S. aureus group. Many DIE-miRNAs, such as bta-miR-144, bta-miR-451 and bta-miR-7863, might be the useful biomarkers of mastitis caused by E. coli and S. aureus. In addition, target genes of the DIE-miRNAs were predicted. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analysis indicated that these DIE-miRNAs are likely involved in many immune signaling pathways, including the Toll-like receptor signaling pathways, MAPK signaling pathway, cell adhesion molecules, TGF-β signaling pathway, leukocyte trans endothelial migration, cytokine-cytokine receptor interaction, and chemokine signaling pathways. This study has provided supportive evidence that miRNAs may serve as diagnostic biomarkers of mastitis in dairy cows, and suggests potentially of effective strategies to combat mastitis.

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Section: Discussionsupporting

confidence: 72%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Comparison of microRNA Profiles between Bovine Mammary Glands Infected with Staphylococcus aureus and Escherichia coli

et al. 2018

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“…coli and S . aureus -infected mammary tissues and the results show that there are multiple differentially expressed miRNAs in infected mammary tissues 10 , 22 , 23 .…”

Section: Discussionmentioning

confidence: 85%

Expression profiling of peripheral blood miRNA using RNAseq technology in dairy cows with Escherichia coli-induced mastitis

Luoreng

Wang

Mei

et al. 2018

Sci Rep

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E. coli is the main causative agent of mastitis in dairy cows, but the mechanism of molecular regulation underlying the occurrence and development of mastitis has not yet been fully elucidated. In this study, an E. coli-induced mastitis model was created and RNASeq technology was used to measure the miRNA expression profiles at different times post-infection (0, 1, 3, 5, 7 dpi), as well as to screen for differentially expressed miRNA. The results show detection of 2416 miRNAs, including 628 known miRNAs and 1788 newly discovered miRNAs. A total of 200 differentially expressed miRNAs were found at different time points. Bioinformatics analysis showed that these differentially expressed miRNAs may regulate the occurrence and development of mastitis in dairy cows through seven signal transduction pathways, namely cytokine-cytokine receptor interaction, MAPK signaling pathway, chemokine signaling pathway, leukocyte transendothelial migration, T cell receptor signaling pathway, Toll-like receptor signaling pathway, and cell adhesion molecules. In addition, bta-miR-200a, bta-miR-205, bta-miR-122, bta-miR-182 and the newly discovered conservative_15_7229 might be involved in immune process in late stage of E. coli-induced mastitis. The results of this study lay the foundation for molecular network analysis of mastitis and molecular breeding of dairy cows.

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“…Next‐generation sequencing has been widely used in bovine mastitis‐related small RNA transcriptome studies, and commonly reported experimental designs include artificial bacterial challenge in an in vivo or in vitro model and the investigation of differentially expressed miRNAs . Rather than bacterial challenge experiments, we analyzed milk samples from naturally infected cattle.…”

Section: Discussionmentioning

confidence: 99%

Bovine milk transcriptome analysis reveals microRNAs and RNU2 involved in mastitis

Lai

Lai²,

Rahman

et al. 2019

The FEBS Journal

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Mastitis is a common inflammatory infectious disease in dairy cows. To understand the microRNA (miRNA) expression profile changes during bovine mastitis, we undertook a genome-wide miRNA study of normal milk and milk that tested positive on the California mastitis test for bovine mastitis (CMT+). Twenty-five miRNAs were differentially expressed (23 miRNAs upregulated and two downregulated) during bovine mastitis relative to their expression in normal milk. Upregulated mature miR-1246 probably derived from a U2 small nuclear RNA rather than an miR-1246 precursor. The significantly upregulated miRNA precursors and RNU2 were significantly enriched on bovine chromosome 19, which is homologous to human chromosome 17. A gene ontology analysis of the putative mRNA targets of the significantly upregulated miRNAs showed that these miRNAs were involved in binding target mRNA transcripts and regulating target gene expression, and a Kyoto Encyclopedia of Genes and Genomes pathway analysis showed that the upregulated miRNAs were predominantly related to cancer and immune system pathways. Three novel miRNAs were associated with bovine mastitis and were relatively highly expressed in milk. We confirmed that one of the novel mastitis-related miRNAs was significantly upregulated using a digital PCR system. The differentially expressed miR-NAs were involved in human cancers, infections, and immune-related diseases. The genome-wide analysis of miRNA profiles in this study provides insight into bovine mastitis and inflammatory diseases. DatabasesThe miRNAseq generated for this study can be found in the Sequence Read Archive (SRA) under BioProject Number PRJNA421075 and SRA Study Number SRP126134 (https://www. ncbi.nlm.nih.gov/bioproject/PRJNA421075).

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Expression patterns of miR-146a and miR-146b in mastitis infected dairy cattle

Cited by 36 publications

References 15 publications

Comparison of microRNA Profiles between Bovine Mammary Glands Infected with Staphylococcus aureus and Escherichia coli

Comparison of microRNA Profiles between Bovine Mammary Glands Infected with Staphylococcus aureus and Escherichia coli

Expression profiling of peripheral blood miRNA using RNAseq technology in dairy cows with Escherichia coli-induced mastitis

Bovine milk transcriptome analysis reveals microRNAs and RNU2 involved in mastitis

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