Expression Patterns of Micro-RNAs 146a, 181a, and 155 in Subacute Sclerosing Panencephalitis

Abstract

Subacute sclerosing panencephalitis is caused by persistent brain infection of mutated virus, showing inflammation, neurodegeneration, and demyelination. Although many factors are emphasized in the pathogenesis of subacute sclerosing panencephalitis, the exact mechanism of neurodegeneration remains unknown. Micro-RNAs are small, noncoding RNAs that regulate gene expression at the posttranscriptional levels. Micro-RNAs are essential for normal immune system development; besides they are also implicated in the pathogenesis of many chronic inflammatory disorders. The aim of this study is to investigate the expression patterns of micro-RNAs 146a, 181a, and 155 in peripheral blood mononuclear cells of patients with subacute sclerosing panencephalitis. We enrolled 39 patients with subacute sclerosing panencephalitis and 41 healthy controls. Quantitative analysis of micro-RNAs 146a, 181a, and 155 were performed using specific stem-loop primers followed by real-time polymerase chain reaction. All of 3 micro-RNAs were upregulated in subacute sclerosing panencephalitis patients. In addition, the level of micro-RNA 155 expression was higher in stage 3 patients. But, micro-RNA 146a and 181a expression levels showed no association or correlation with clinically relevant data. Alteration of peripheral blood mononuclear cell micro-RNAs in subacute sclerosing panencephalitis may shed new light on the pathogenesis of disease and may contribute to the aberrant systemic rise in mRNA levels in subacute sclerosing panencephalitis.

MiRNA-124 is a link between measles virus persistent infection and cell division of human neuroblastoma cells

“…Recently, Yis et al described the induction of three miRNAs (miRNAs 146a, 181a, and 155) in peripheral blood mononuclear cells of patients with SSPE, with no prognostic significance [ 36 ]. We examined these miRNAs in our neuroblastoma cells.…”

miR-155 Inhibits Nucleus Pulposus Cells’ Degeneration through Targeting ERK 1/2

“…The expression of miR-155 increased in activated B cells, T cells, and monocyte/macrophage [8]. In addition, the study had found that miR-155 exists in synovioblast and mononuclear cells in rheumatoid arthritis [9]. Therefore, miR-155 plays an important role in inducing inflammatory reaction and immune response.…”

Small Non-coding RNA Expression Following Respiratory Syncytial Virus or Measles Virus Infection of Neuronal Cells

“…Other studies have shown that the pattern of miR expression is modified following RSV infection of nasal epithelial cells (Inchley et al, 2015;Hasegawa et al, 2018), and PBMCs from children (Wang et al, 2017). Deregulated miR expression occurs in MeV-infected human neuroblastoma cells and PBMCs (Inchley et al, 2015;Yis et al, 2015;Naaman et al, 2017;Hasegawa et al, 2018), as well as in PBMCs from RSV-infected children (Wang et al, 2017). It is known that MeV persistence in neuroblastoma cells is assisted by the downregulation of CDK6, a component of cell cycle progression regulated by miRNA-124 (Riddell et al, 2007;Naaman et al, 2017;Griffin et al, 2018).…”