Expression patterns of immune genes in long-term cultured dental stem cells

Jayaraman, Pukana; Govindasamy, Vijayendran; Gnanasegaran, Nareshwaran; Kunasekaran, Wijenthiran; Vasanthan, Punitha; Musa, Sabri; Kasim, Noor Hayaty Abu

doi:10.1007/s00784-015-1497-1

Cited by 14 publications

(12 citation statements)

References 31 publications

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“…A previous study showed that BMMSCs maintain stable properties in vitro during the first 6‐8 passages of expansion culture and enter a phase of senescence within 7‐12 passages . As for dental MSCs, Pukana Jayaraman considered P9 cells were long‐term cultured dental stem cells . Studies about DPSCs also showed that the differentiation potential of DPSCs is restricted at the 9th passage and senescence of DPSCs may occur after the 10th passage .…”

Section: Discussionmentioning

confidence: 99%

A comprehensive study on donor‐matched comparisons of three types of mesenchymal stem cells‐containing cells from human dental tissue

Xing

Zhang

et al. 2018

J of Periodontal Research

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Background and Objective Mesenchymal stem cells (MSCs) have been widely used in tissue engineering, such as for regenerating the supporting structures of teeth destroyed by periodontal diseases. In recent decades, dental tissue‐derived MSCs have drawn much attention owing to their accessibility, plasticity and applicability. Dental pulp stem cells (DPSCs), periodontal ligament stem cells (PDLSCs) and gingival MSCs (GMSCs) are the most readily available MSCs among all types of dental MSCs. The purpose of this study was to comprehensively compare the characteristics of MSCs from dental pulp (DP), periodontal ligament (PDL) and gingiva (G) in vitro and thus provide insight into optimizing the performance of cells and seed cell selection strategies for tissue regeneration. Materials and Methods In this study, patient‐matched (n = 5) cells derived from DP, PDL and G which, respectively, contained DPSCs, PDLSCs and GMSCs were evaluated using multiple methods in terms of their proliferation, senescence, apoptosis, multilineage differentiation and stemness maintenance after long‐term passage. Results Mesenchymal stem cells‐containing cells from G (MSCs/GCs) showed superior proliferation capability, whereas patient‐matched MSCs‐containing cells from PDL (MSCs/PDLCs) exhibited excellent osteogenic and adipogenic differentiation ability; MSCs‐containing cells from DP (MSCs/DPCs) achieved mediocre results in both aspects. In addition, MSCs/GCs were the least susceptible to senescence, while MSCs/PDLCs were the most prone to ageing. Furthermore, the biological properties of these three types of cells were all affected after long‐term in vitro culture. Conclusion These three types of dental MSCs showed different biological characteristics. MSCs/PDLCs are the best candidate cells for bone regeneration, but the application of MSCs/PDLCs might be limited to certain number of passages. Improving the differentiation of MSCs/GCs remains the key issue regarding their application in tissue engineering.

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Section: Discussionmentioning

confidence: 99%

A comprehensive study on donor‐matched comparisons of three types of mesenchymal stem cells‐containing cells from human dental tissue

Xing

Zhang

et al. 2018

J of Periodontal Research

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“…However, PDLSC proliferation remains slow under the current culture conditions. More importantly, these cells inevitably undergo replicative senescence, resulting in potential cellular phenotypic changes and/or loss of cell stemness (Kim et al 2009;Jayaraman et al 2016). Indeed, there is evidence that the in vitro lifespan of PDL-derived cells is shorter than that of other cell types in connective tissues (Sawa et al 2000).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, maintaining the stemness of PDLSCs, particularly with regard to their migration, proliferation and differentiation, has become a vital issue that must be carefully addressed before these cells enter the clinical testing stage . Clearly, the current in vitro system cannot replicate the desired in vivo environment, which consists in various factors, such as oxygen tension, biochemical molecules and mechanical forces (Ashton et al 2011), generally leading to low-quality cellular products with impaired proliferation, early senescence and/or the loss of regenerative potential (Sawa et al 2000;Kim et al 2009;Jayaraman et al 2016).…”

Section: Introductionmentioning

confidence: 99%

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

et al. 2016

Cell Tissue Res

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In this study, we extensively screened the in vitro and in vivo effects of PDLSCs following short-term inflammatory and/or hypoxic pretreatments. We found that the 24-h hypoxic pretreatment of PDLSCs significantly enhanced cell migration and improved cell surface CXCR4 expression. In addition, hypoxia-pretreated PDLSCs exhibited improved cell colony formation and proliferation. Cells that were dually stimulated also formed more colonies compared to untreated cells but their proliferation did not increase. Importantly, the hypoxic pretreatment of PDLSCs enhanced cell differentiation as determined by elevated RUNX-2 and ALP protein expression. In this context, the inflammatory stimulus impaired cell OCN protein expression, while dual stimuli led to decreased RUNX-2 and OCN mRNA levels. Although preconditioning PDLSCs with inflammatory and/or hypoxic pretreatments resulted in no differences in the production of matrix proteins, hypoxic pretreatment led to the generation of thicker cell sheets; the inflammatory stimulus weakened the ability of cells to form sheets. All the resultant cell sheets exhibited clear bone regeneration following ectopic transplantation as well as in periodontal defect models; the amount of new bone formed by hypoxia-preconditioned cells was significantly greater than that formed by inflammatory stimulus- or dual-stimuli-treated cells or by nonpreconditioned cells. The regeneration of new cementum and periodontal ligaments was only identified in the hypoxia-stimulus and no-stimulus cell groups. Our findings suggest that PDLSCs that undergo short-term hypoxic pretreatment show improved cellular behavior in vitro and enhanced regenerative potential in vivo. The preconditioning of PDLSCs via combined treatments or an inflammatory stimulus requires further investigation.

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“…The application of hDPSCs as a model of study was useful due to its multipotentiality in differentiating into several cell types (Pierdomenico et al, 2005) and to present a low immunogenicity (Gronthos, Mankani, Brahim, Robey, & Shi, 2000). In addition, hDPSCs are mesenchymal stem cells (MSCs) that are easy to isolate and culture, and it does not face ethical barriers since its source is a tissue that would normally be discarded after, for example, dental extractions (Arany et al, 2014;de Sá Silva et al, 2012;Ding, Niu, & Liu, 2015;Gronthos et al, 2000;Jayaraman et al, 2016). associated with growth factors (Diniz et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Photobiomodulation therapy and vitamin C on longevity of cell sheets of human dental pulp stem cells

Pedroni

Diniz

Abe

et al. 2018

Journal Cellular Physiology

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Photobiomodulation therapy (PBMT) can improve processes relevant to tissue regeneration, such as survival, proliferation, migration, and differentiation of cells, including stem cells. Thus, PBMT could be applied as auxiliary therapy for tissue regeneration. Cell sheets (CSs) induced by vitamin C (VC) can generate large amount of cells, which would also be useful for tissue regeneration. VC and PBMT cause opposite effects on cell metabolism (e.g., VC is antioxidative, and PBMT generates reactive oxygen species); however, hDPSC CSs were formed when VC and PBMT+VC were applied. Thus, this study showed that PBMT does not interfere with the formation of cell sheets induced by VC. Additionally, PBMT improved the functional differentiation of the cells isolated from the CSs. Thus, due to the clinical benefits of PBMT, the association of this therapy with cell sheets seems promising for future applications in tissue regeneration.

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Expression patterns of immune genes in long-term cultured dental stem cells

Abstract: A complete biological characterization covering all major aspects including immune properties should be made as prerequisite criteria prior to the use of long-term cultured stem cells in clinical settings.

Cited by 14 publications

References 31 publications

A comprehensive study on donor‐matched comparisons of three types of mesenchymal stem cells‐containing cells from human dental tissue

A comprehensive study on donor‐matched comparisons of three types of mesenchymal stem cells‐containing cells from human dental tissue

Effects of short-term inflammatory and/or hypoxic pretreatments on periodontal ligament stem cells: in vitro and in vivo studies

Photobiomodulation therapy and vitamin C on longevity of cell sheets of human dental pulp stem cells

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