Expression patterns of Fcγ receptors, HLA-DR and selected adhesion molecules on monocytes from normal and HIV-infected individuals

Locher, Christopher P.; Vanham, Guido; Kestens, Luc; Kruger, Mariana; Ceuppens, Jan L.; Vingerhoets, J.; Gigase, P.

doi:10.1111/j.1365-2249.1994.tb06616.x

Cited by 57 publications

(11 citation statements)

References 37 publications

(38 reference statements)

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“…An expansion of CD14 + CD16 + monocytes has been observed after HIV-1 infection [10]–[12] and other inflammatory conditions, while numbers of CD14 ++ monocytes do either not change [11], [13], or decrease slightly [10], in agreement with our observations. It has been assumed that CD14 + CD16 + monocytes are a distinct “proinflammatory” subset of the monocyte lineage [14].…”

Section: Discussionsupporting

confidence: 92%

Sialoadhesin (CD169) Expression in CD14+ Cells Is Upregulated Early after HIV-1 Infection and Increases during Disease Progression

et al. 2007

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BackgroundSialoadhesin (CD169, siglec-1 or Sn) is an activation marker seen on macrophages in chronic inflammatory diseases and in tumours, and on subsets of tissue macrophages. CD169 is highly expressed by macrophages present in AIDS-related Kaposi's sarcoma lesions. It is also increased on blood monocytes of HIV-1 infected patients with a high viral load despite antiretroviral treatment.Methodology/Principal FindingsWe investigated expression of sialoadhesin in untreated HIV-1 and HHV-8 infected patients, by real-time PCR and FACS analysis to establish its expression in relation to infection and disease progression. Patients analysed were either HIV-1 seroconverters (n = 7), in the chronic phase of HIV-1 infection (n = 21), or in the AIDS stage (n = 58). Controls were HHV-8 infected, but otherwise healthy individuals (n = 20), and uninfected men having sex with men (n = 24). Sialoadhesin mRNA was significantly elevated after HIV-1, but not HHV-8 infection, and a further increase was seen in AIDS patients. Samples obtained around HIV-1 seroconversion indicated that sialoadhesin levels go up early in infection. FACS analysis of PBMCs showed that sialoadhesin protein was expressed at high levels by approximately 90% of CD14+ and CD14+CD16+cells of HIV-1+ patients with a concomitant 10-fold increase in sialoadhesin protein/cell compared with uninfected controls.Conclusions/SignificanceWe have shown that sialoadhesin is induced to high levels on CD14+ cells early after HIV-1 infection in vivo. The phenotype of the cells is maintained during disease progression, suggesting that it could serve as a marker for infection and probably contributes to the severe dysregulation of the immune system seen in AIDS.

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Section: Discussionsupporting

confidence: 92%

Sialoadhesin (CD169) Expression in CD14+ Cells Is Upregulated Early after HIV-1 Infection and Increases during Disease Progression

et al. 2007

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“…This cell subset is expanded in several inflammatory diseases such as sepsis, HIV infection, and tuberculosis or in patients with acute or chronic infection undergoing hemodialysis (Nockher and Scherberich 1998) and is selectively suppressed by glucocorticoid therapy (Nockher and Scherberich 1998;Locher et al 1994;Vanham et al 1996;Fingerle-Rowson et al 1998). As this population is responsible for production of higher levels of TNF than the CD14+ monocytes (Calandra and Roger 2003), the increase in TNF level is the same in both groups in our study.…”

Section: Discussionsupporting

confidence: 54%

The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia

Bakry

Sayed

et al. 2015

Comp Clin Pathol

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Paravertebral block is a method of regional block used to make pain relief and decrease systemic analgesic requirements for patients undergoing breast cancer surgery. This work aimed to evaluate the effect of paravertebral block on monocyte phenotype and their functions after surgery for cancer breast patients. One hundred patients which the American Society of Anesthesia (ASA) I-III planned for breast cancer surgery were allocated into two groups. Group A (n=50) received general anesthesia in a standard technique. Group B (n=50) received paravertebral block T2-T6 20 ml plain bupivacaine 0.5 % plus 10 ml 2 % lidocaine prior to induction of general anesthesia. Two samples were collected from all patients preoperatively as a baseline and 24 h after surgery. Flow cytometric monocyte phenotype analysis (CD14, HLA-DR, and CD86) was performed. In addition, tumor necrosis factor alpha (TNF-α), monocyte inhibitory factor (MIF), and macrophage chemoattractant protein (MCP-1) were measured in both groups. There were no significant differences between the two groups as regard to age, weight, height, and duration of surgery. One day after surgery, there was significant decrease in all measured markers (CD14, HLA-DR, and CD86) in group A. On the other hand, insignificant decrease in CD 14 and HLA-DR and insignificant increase in CD86 in group B were observed, also 24 h after surgery. As regard to monocyte producing cytokines, significant increase in TNF-α and MIF levels was recorded in both groups after 24 h of surgery; these increases were the same in TNF-α level and more significant in MIF in group A than group B. MCP-1 was decreased insignificantly in group A and significantly in group B. Paravertebral block combined with general anesthesia can significantly reduce monocyte suppression which is an integral part of immunological response following major surgery.

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“…During HIV infection, chronic innate immune activation with expansion of proinflammatory monocyte subpopulations and related cytokine production is only partially returned to normal levels upon the initiation of cART (2,28,(32)(33)(34). In the studies reported in this article, we show that, irrespective of cART, there is also an increase in the percentage of monocytes expressing Glut1 in HIV + individuals when compared with monocytes from HIV 2 individuals.…”

Section: Discussionmentioning

confidence: 53%

Glucose Transporter 1–Expressing Proinflammatory Monocytes Are Elevated in Combination Antiretroviral Therapy–Treated and Untreated HIV+ Subjects

Palmer

Anzinger

Zhou

et al. 2014

The Journal of Immunology

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Monocyte activation during HIV-1 infection is associated with increased plasma levels of inflammatory markers and increased risk for premature development of age-related diseases. Because activated monocytes primarily use glucose to support cellular metabolism, we hypothesized that chronic monocyte activation during HIV-1 infection induces a hypermetabolic response with increased glucose uptake. To test this hypothesis, we evaluated glucose transporter 1 (Glut1) expression and glucose uptake by monocyte subpopulations in HIV-seropositive (HIV+) treatment-naive individuals (n = 17), HIV+ individuals on combination antiretroviral therapy with viral loads below detection (n = 11), and HIV-seronegative (HIV−) individuals (n = 16). Surface expression of Glut1 and cellular uptake of the fluorescent glucose analog 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2 deoxyglucose were analyzed by flow cytometry on monocyte subpopulations. Irrespective of treatment status, monocytes from HIV+ persons had significantly increased surface expression of Glut1 compared with those from HIV− controls. Nonclassical (CD14+CD16++) and intermediate (CD14++CD16+) monocyte subpopulations showed higher Glut1 expression than did classical (CD14++CD16−) monocytes. Intermediate monocytes from treatment-naive HIV+ individuals also showed increased uptake of 2-(N-(7-nitrobenz-2-oxa-1, 3-diazol-4-yl) amino)-2 deoxyglucose compared with those from HIV− controls. Our results show that HIV infection is associated with increased glucose metabolism in monocytes and that Glut1 expression by proinflammatory monocytes is a potential marker of inflammation in HIV-infected subjects. However, the possibility exists whereby other Gluts such as Glut3 and Glut4 may also support the influx of glucose into activated and inflammatory monocyte populations.

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Expression patterns of Fcγ receptors, HLA-DR and selected adhesion molecules on monocytes from normal and HIV-infected individuals

Cited by 57 publications

References 37 publications

Sialoadhesin (CD169) Expression in CD14+ Cells Is Upregulated Early after HIV-1 Infection and Increases during Disease Progression

Sialoadhesin (CD169) Expression in CD14+ Cells Is Upregulated Early after HIV-1 Infection and Increases during Disease Progression

The immunological role of monocytes and their cytokines in patients with breast cancer undergoing paravertebral analgesia

Glucose Transporter 1–Expressing Proinflammatory Monocytes Are Elevated in Combination Antiretroviral Therapy–Treated and Untreated HIV+ Subjects

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