Expression patterns and role of the CadF protein in<i>Campylobacter jejuni</i>and<i>Campylobacter coli</i>

Krause-Gruszczynska, Malgorzata; Alphen, Lieke B. van; Oyarzábal, Omar A.; Alter, Thomas; Hänel, Ingrid; Schliephake, Annette; König, Wolfgang; Putten, Jos P. M. van; Konkel, Michael E.; Backert, Steffen

doi:10.1111/j.1574-6968.2007.00802.x

Cited by 58 publications

(43 citation statements)

References 22 publications

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“…2A and B). This protein is a 37-kDa OMP that is essential for C. jejuni colonization of chickens (12,14,17,25,26,51). A reactive band (band 11), corresponding to a protein with a molecular mass of 37 kDa, was observed in the OMP extracts from the C. jejuni S3B and 81-176 strains using each of the nine C. jejuni S3B-SPF sera ( Fig.…”

Section: Resultsmentioning

confidence: 99%

Identification of Campylobacter jejuni Proteins Recognized by Maternal Antibodies of Chickens

Shoaf-Sweeney

Larson

Tang

et al. 2008

Appl Environ Microbiol

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Campylobacter jejuni is one of the leading bacterial causes of food-borne gastroenteritis. Infection with C. jejuni is frequently acquired through the consumption of undercooked poultry or foods cross-contaminated with raw poultry. Given the importance of poultry as a reservoir for Campylobacter organisms, investigators have performed studies to understand the protective role of maternal antibodies in the ecology of Campylobacter colonization of poultry. In a previous study, chicks with maternal antibodies generated against the S3B strain of C. jejuni provided protection against Campylobacter colonization (O. Sahin, N. Luo, S. Huang, and Q. Zhang, Appl. Environ. Microbiol. 69:5372-5379, 2003). We obtained serum samples, collectively referred to as the C. jejuni S3B-SPF sera, from the previous study. These sera were determined to contain maternal antibodies that reacted against C. jejuni whole-cell lysates as judged by enzyme-linked immunosorbent assay. The antigens recognized by the C. jejuni S3B-SPF antibodies were identified by immunoblot analysis, coupled with mass spectrometry, of C. jejuni outer membrane protein extracts. This approach led to the identification of C. jejuni proteins recognized by the maternal antibodies, including the flagellin proteins and CadF adhesin. In vitro assays revealed that the C. jejuni S3B-SPF sera retarded the motility of the C. jejuni S3B homologous strain but did not retard the motility of a heterologous strain of C. jejuni (81-176). This finding provides a possible mechanism explaining why maternal antibodies confer enhanced protection against challenge with a homologous strain compared to a heterologous strain. Collectively, this study provides a list of C. jejuni proteins against which protective antibodies are generated in hens and passed to chicks.

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Section: Resultsmentioning

confidence: 99%

Identification of Campylobacter jejuni Proteins Recognized by Maternal Antibodies of Chickens

Shoaf-Sweeney

Larson

Tang

et al. 2008

Appl Environ Microbiol

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“…Several studies identified some adhesion factors of C. jejuni such as CadF (fibronectin-binding outer membrane protein), CapA (the autotransporter), and PEB1 (periplasmic binding protein) (Pei and Blaser, 1993;Konkel et al, 1997). CadF, mediating cell adhesion by attaching to fibronectin, can be considered a key virulence-associated factor because the pathogenicity is significantly reduced in cadF mutants (Ziprin et al, 1999;Krause-Gruszczynska et al, 2007b;Dastia et al, 2010). Furthermore, the invasion ability of Campylobacter is one of the virulence-associated factors because several studies performed on different animal models revealed that mucosal damage at early stages of infection results from invasion of C. jejuni into epithelial cells in the colon (Humphrey et al, 1985;Field et al, 1986;Babakhani et al, 1993).…”

Section: Adhesion and Invasionmentioning

confidence: 98%

“…CadF, CapA, PEB1 Fauchere et al (1986), Konkel et al (1997), Ziprin et al (1999), Krause-Gruszczynska et al (2007b), Dastia et al (2010) Invasion Invasion of C. jejuni results in mucosal damage initially in colon.…”

Section: Introductionmentioning

confidence: 97%

Clinical relevance of infections with zoonotic and human oral species of Campylobacter

Lee

et al. 2016

J Microbiol.

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Genus Campylobacter has been recognized as a causative bacterial agent of animal and human diseases. Human Campylobacter infections have caused more concern. Campylobacters can be classified into two groups in terms of their original host: zoonotic and human oral species. The major zoonotic species are Campylobacter jejuni and Campylobacter coli, which mostly reside in the intestines of avian species and are transmitted to humans via consumption of contaminated poultry products, thus causing human gastroenteritis and other diseases as sequelae. The other campylobacters, human oral species, include C. concisus, C. showae, C. gracilis, C. ureolyticus, C. curvus, and C. rectus. These species are isolated from the oral cavity, natural colonization site, but have potential clinical relevance in the periodontal region to varying extent. Two species, C. jejuni and C. coli, are believed to be mainly associated with intestinal diseases, but recent studies suggested that oral Campylobacter species also play a significant role in intestinal diseases. This review offers an outline of the two Campylobacter groups (zoonotic and human oral), their virulence traits, and the associated illnesses including gastroenteritis.

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“…Our results showed that the mixed infections usually had a predominant organism with up to 4 logs more bacteria than the other organism. Relevant to quantitation is the fact that most of our targets were single-copy chromosomal genes (aerolysin, cadF, toxR, lysP, and invA) (15,22,25,48). The ipaH target is likely to be less useful, because its copy numbers vary between species (42,47).…”

Section: Discussionmentioning

confidence: 99%

Simultaneous Detection of Six Diarrhea-Causing Bacterial Pathogens with an In-House PCR-Luminex Assay

et al. 2012

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Diarrhea can be caused by a range of pathogens, including several bacteria. Conventional diagnostic methods, such as culture, biochemical tests, and enzyme-linked immunosorbent assay (ELISA), are laborious. We developed a 7-plex PCR-Luminex assay to simultaneously screen for several of the major diarrhea-causing bacteria directly in fecal specimens, including pathogenic Aeromonas, Campylobacter jejuni, Campylobacter coli, Salmonella, Shigella, enteroinvasive Escherichia coli (EIEC), Vibrio, and Yersinia. We included an extrinsic control to verify extraction and amplification. The assay was first validated with reference strains or isolates and exhibited a limit of detection of 10 3 to 10 5 CFU/g of stool for each pathogen as well as quantitative detection up to 10 9 CFU/g. A total of 205 clinical fecal specimens from individuals with diarrhea, previously cultured for enteric pathogens and tested for Campylobacter by ELISA, were evaluated. Using these predicate methods as standards, sensitivities and specificities of the PCR-Luminex assay were 89% and 94% for Aeromonas, 89% and 93% for Campylobacter, 96% and 95% for Salmonella, 94% and 94% for Shigella, 92% and 97% for Vibrio, and 100% and 100% for Yersinia, respectively. All discrepant results were further examined by singleplex real-time PCR assays targeting different gene regions, which revealed 89% (55/62 results) concordance with the PCR-Luminex assay. The fluorescent signals obtained with this approach exhibited a statistically significant correlation with the cycle threshold (C T ) values from the cognate real-time PCR assays (P < 0.05). This multiplex PCR-Luminex assay enables sensitive, specific, and quantitative detection of the major bacterial causes of gastroenteritis.

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Expression patterns and role of the CadF protein inCampylobacter jejuniandCampylobacter coli

Cited by 58 publications

References 22 publications

Identification of Campylobacter jejuni Proteins Recognized by Maternal Antibodies of Chickens

Identification of Campylobacter jejuni Proteins Recognized by Maternal Antibodies of Chickens

Clinical relevance of infections with zoonotic and human oral species of Campylobacter

Simultaneous Detection of Six Diarrhea-Causing Bacterial Pathogens with an In-House PCR-Luminex Assay

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