Expression patterns and promoter characteristics of the Vitis quinquangularis VqSTS36 gene involved in abiotic and biotic stress response

Abstract

Resveratrol is a stilbene compound that is synthesized by plants in response to biotic stress and has been linked to health benefits associated with the consumption of certain foods and food products, such as grapes and wine. The final step in the biosynthesis of resveratrol is catalyzed by the enzyme stilbene synthase (STS). Here, we assessed the expression of two STS genes (VqSTS36 and VpSTS36) from the wild grape species Vitis quinquangularis (accession 'Shang-24'; powdery mildew (PM) resistant) and Vitis pseudoreticulata (accession 'Hunan-1'; PM susceptible) following infection by Uncinula necator (Schw.) Burr, the causal agent of PM disease. Some correlation was observed between the relative levels of STS36 transcript and disease resistance. We also cloned the 5' upstream sequence of both VpSTS36 and VqSTS36 and generated a series of 5' VqSTS36 promoter deletions fused to the GUS reporter gene in order to analyze expression in response to wounding, the application of exogenous stress-associated hormones, and biotic stress in tobacco leaves. The promoter was shown to be induced by the hormone salicylic acid (SA), inoculation with the fungal pathogen Erysiphe cichoracearum, and by wounding. These results suggest that VqSTS36 is regulated by biotic stresses and that it plays an important role in mediating disease resistance in grape.

Comparative Expression Profiling of Snf2 Family Genes During Reproductive Development and Stress Responses in Rice

“…These included DRE or binding site for MYC transcription factor for dehydration responsive elements ( Narusaka et al, 2003 ; Tran et al, 2004 ). LTR for low temperature-responsive element, STRE or TC-rich motifs for stress responses, CCAAT box and MYB binding sites responsive to drought treatments, Box S and WUN motif for wounding and pathogen elicitation response elements ( Xu et al, 2011 ; Yin et al, 2017 ). Several phytohormone responsive elements were also observed in the promoter regions, such as TCA element for salicylic acid responses, ERE as ethylene responses, CGTCA- or TGACG-motif for MeJA responsive element, ABRE as ABA-responsive element, TGA-element or AuxRR core or AuxRE for auxin responses, GARE-motif, and TATC-box for gibberellin responses.…”

Impact of Environmental Factors on Stilbene Biosynthesis

“…Induction of VvSTS in canes from grapevine Cabernet Franc has been recorded during the first 4 weeks of storage by Houillé and coworkers [217], indicating that grapevine wood is still transcriptionally active after pruning. In 2017, Yin et al [220] observed a significant induction by wounding stress of VqSTS36 promoter activity in Vitis quadrangularis L. In 2018, Vannozzi and co-workers [218] reported that in response to wounding the transcript level of VviSTS29, -41 and -48 gradually increases, coupled with the induction of WRKY and R2R3-MYB transcription factors. Four WRKY genes, namely VviWRKY03, VviWRKY24, VviWRKY43, and VviWRKY53, were thus reported for being involved in the regulation of the stilbene biosynthetic pathway.…”