Expression Patterns and Potential Biological Roles of Dip2a

Zhang, Luqing; Mabwi, Humphrey A.; Palange, Norberto J.; Jia, Rui-Rui; Ma, Jun; Bah, Fatoumata Binta; Sah, Rajiv Kumar; Li, Dan; Wang, Daji; Togo, Jacques; Jin, Honghong; Ban, Luying; Feng, Xuechao; Zheng, Yaowu

doi:10.1371/journal.pone.0143284

Cited by 28 publications

(30 citation statements)

References 18 publications

(26 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Samples were treated with Mouse Direct PCR Kits. For LacZ insertion, the following primers were used: Forward primer: 5 -ACCA CACCTCCTGCTGTATAC-3 and Reverse primer: 5 -ACGA CGGGATCATCGCGAGCCAT-3 (Zhang et al, 2015). For LacZ homozygote and heterozygote identification, the following primers were used: Forward primer: 5 -AGTTAAGGCTGAGC ATGGTGGGA-3 and Reverse primer: 5 -TAGGGCTCTCAC AGATCAGAGCT-3 .…”

Section: Genotypingmentioning

confidence: 99%

“…Mammals express three DIP2 genes (DIP2A, DIP2B, and DIP2C) each of which shares highly conserved functional domains and have similar roles in acetyl-coenzyme (acetyl-CoA) synthesis . In adult mice, DIP2A is expressed in multiple brain regions including the neocortex, hippocampus, amygdala, and cerebellum (Zhang et al, 2015). In humans, DIP2A is located on chromosome 21q22.3 and is associated with developmental delay (Yu et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

DIP2B Interacts With α-Tubulin to Regulate Axon Outgrowth

Xing

Zhang

Sun

et al. 2020

Front. Cell. Neurosci.

View full text Add to dashboard Cite

Axonal development is essential to the establishment of neuronal morphology and circuitry, although the mechanisms underlying axonal outgrowth during the early developmental stages remain unclear. Here, we showed that the conserved disco-interacting protein B (DIP2B) which consists of a DMAP1 domain and a crotonobetaine/carnitine CoA ligase (Caic) domain, is highly expressed in the excitatory neurons of the hippocampus. DIP2B knockout led to excessive axonal outgrowth but not polarity at an early developmental stage. Furthermore, the loss of DIP2B inhibited synaptic transmission for both spontaneous and rapid release in cultured hippocampal neurons. Interestingly, DIP2B function during axonal outgrowth requires tubulin acetylation. These findings reveal a new conserved regulator of neuronal morphology and provide a novel intervention mechanism for neurocognitive disorders.

show abstract

Section: Genotypingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

DIP2B Interacts With α-Tubulin to Regulate Axon Outgrowth

Xing

Zhang

Sun

et al. 2020

Front. Cell. Neurosci.

View full text Add to dashboard Cite

show abstract

“…However, we did not find differences in the expression of the selected genes. However, we are aware that these genes represent only a small amount of those that can interfere with proper embryo development and implantation, since a number of studies have been published showing the importance of a number of other genes in this period (Vogt, Meglicki, Hartung, Borsuk, & Behr, 2012;Zhang et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

Sleep deprivation decreases the reproductive capacity by affecting the arrival of morulas in the uterus

Calegare

Azzolini

Vallim

et al. 2019

Genesis

View full text Add to dashboard Cite

Summary A previous animal study by our group found that sleep deprivation during preimplantation was associated with decreased pregnancy maintenance. Given its impact on human society, we aimed in the current study to assess whether sleep deprivation affects blastocyst gene expression and/or the implantation process. For this, pregnant mice (gestational day 0 [GD 0]) were assigned into paradoxical sleep deprivation (SD, 72 hr; multiple platform method) and, a control (CT) group. Animals were euthanized on GD 3.5 and blood, uterus (embryos) and fallopian tube were collected. Then, 89% of CT presented blastocysts in the uterus versus 25% from SD group. Compared to CT, SD presented lighter relative uterus weight, increased plasma concentrations of corticosterone and testosterone, decreased concentrations of progesterone and luteinizing hormone, but no statistical differences in plasma concentrations of 17β‐estradiol and follicle stimulating hormone. There were no differences in uterus and blastocyst gene expression related to embryo implantation and development, and no alteration in blastocysts global DNA methylation. Considering this, the decreased pregnancy maintenance after sleep deprivation seems not to be associated with implantation losses or developmental problems related to the blastocysts. It is likely that complications in morula development and/or its movement through the fallopian tubes affect the pregnancy rate, since only 25% of SD females presented a blastocyst on the GD 3.5. In fact, three out of four females without blastocysts in the uterus presented morula in the fallopian tubes due to a phase delay. Additionally, we suggest that the observed hormonal changes may play a role in this outcome.

show abstract

“…RING finger protein 207 (RNF207) was heavy labelled in five of the CCM and is related to shortened action potential duration in ventricular cardiomyocytes in neonatal rabbits and the knockout of this gene also significantly slows conduction in the ventriculum of the heart of developing zebra fish [53]. Another protein heavy labelled in three CCM was disco interacting protein 2A (DIP2A), which has been previously described as widely expressed in ectoderm-derived tissues in developing embryos and is a potential receptor of FSTL1 and its protective role of cardiomyocytes [54]. This protein, FSTL1, was only detected as light labelled in the CCM from one of the conceptuses in our study.…”

Section: De Novo Synthesised Proteins In Conceptus-conditioned Mediamentioning

confidence: 99%

Protein Synthesis by Day 16 Bovine Conceptuses during the Time of Maternal Recognition of Pregnancy

Estepa

Tinning

Vasconcelos

et al. 2020

IJMS

View full text Add to dashboard Cite

Interferon Tau (IFNT), the conceptus-derived pregnancy recognition signal in cattle, significantly modifies the transcriptome of the endometrium. However, the endometrium also responds to IFNT-independent conceptus-derived products. The aim of this study was to determine what proteins are produced by the bovine conceptus that may facilitate the pregnancy recognition process in cattle. We analysed by mass spectrometry the proteins present in conceptus-conditioned media (CCM) after 6 h culture of Day 16 bovine conceptuses (n = 8) in SILAC media (arginine- and lysine-depleted media supplemented with heavy isotopes) and the protein content of extracellular vesicles (EVs) isolated from uterine luminal fluid (ULF) of Day 16 pregnant (n = 7) and cyclic (n = 6) cross-bred heifers on day 16. In total, 11,122 proteins were identified in the CCM. Of these, 5.95% (662) had peptides with heavy labelled amino acids, i.e., de novo synthesised by the conceptuses. None of these proteins were detected in the EVs isolated from ULF. Pregnancy-associated glycoprotein 11, Trophoblast Kunitz domain protein 1 and DExD-Box Helicase 39A were de novo produced and present in the CCM from all conceptuses and in previously published CCM data following 6 and 24 h. A total of 463 proteins were present in the CCM from all the conceptuses in the present study, and after 6 and 24 h culture in a previous study, while expression of their transcripts was not detected in endometrium indicating that they are likely conceptus-derived. Of the proteins present in the EVs, 67 were uniquely identified in ULF from pregnant heifers; 35 of these had been previously reported in CCM from Day 16 conceptuses. This study has narrowed a set of conceptus-derived proteins that may be involved in EV-mediated IFNT-independent embryo–maternal communication during pregnancy recognition in cattle.

show abstract

Expression Patterns and Potential Biological Roles of Dip2a

Cited by 28 publications

References 18 publications

DIP2B Interacts With α-Tubulin to Regulate Axon Outgrowth

DIP2B Interacts With α-Tubulin to Regulate Axon Outgrowth

Sleep deprivation decreases the reproductive capacity by affecting the arrival of morulas in the uterus

Protein Synthesis by Day 16 Bovine Conceptuses during the Time of Maternal Recognition of Pregnancy

Contact Info

Product

Resources

About