Expression Patterns and Functional Analysis of E3 Ubiquitin Ligase Genes in Rice

zhang, huijuan; Zheng, Dewei; Song, Fengming; Jiang, Ming

doi:10.21203/rs.3.rs-575739/v1

Cited by 2 publications

(1 citation statement)

References 51 publications

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“…Excess ROS are scavenged or detoxified by an efficient antioxidative system that includes both nonenzymic and enzymic antioxidants, such as superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) [44]. Previously, [45] it was demonstrated that drought stress resistance in VyP5CR transgenic Arabidopsis revealed less efficient scavenging of H 2 O 2 as compared WT lines. In the present study, we observed that TaPUB2 and TaPUB3 transgenic line have a more effective antioxidant defense system and less accumulation of ROS (Figure 6I and Figure S2).…”

Section: Discussionmentioning

confidence: 99%

Molecular Characterization of U-box E3 Ubiquitin Ligases (TaPUB2 and TaPUB3) Involved in the Positive Regulation of Drought Stress Response in Arabidopsis

Kim

et al. 2021

IJMS

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Plant U-box E3 ubiquitin ligase (PUB) is involved in various environmental stress conditions. However, the molecular mechanism of U-box proteins in response to abiotic stress in wheat remains unknown. In this study, two U-box E3 ligase genes (TaPUB2 and TaPUB3), which are highly expressed in response to adverse abiotic stresses, were isolated from common wheat, and their cellular functions were characterized under drought stress. Transient expression assay revealed that TaPUB2 was localized in the cytoplasm and Golgi apparatus, whereas TaPUB3 was expressed only in the Golgi apparatus in wheat protoplasts. Additionally, TaPUB2 and TaPUB3 underwent self-ubiquitination. Moreover, TaPUB2/TaPUB3 heterodimer was identified in yeast and the cytoplasm of wheat protoplasts using a pull-down assay and bimolecular fluorescence complementation analysis. Heterogeneous overexpression of TaPUB2 and TaPUB3 conferred tolerance to drought stress. Taken together, these results implied that the heterodimeric form of U-box E3 ubiquitin ligases (TaPUB2/TaPUB3) responded to abiotic stress and roles as a positive regulator of drought stress tolerance.

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Section: Discussionmentioning

confidence: 99%

Molecular Characterization of U-box E3 Ubiquitin Ligases (TaPUB2 and TaPUB3) Involved in the Positive Regulation of Drought Stress Response in Arabidopsis

Kim

et al. 2021

IJMS

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Paenibacillus terrae NK3-4 regulates the transcription of growth-related and stress resistance-related genes in rice

Liu

et al. 2023

Genome

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Paenibacillus terrae NK3-4 is a plant growth-promoting rhizobacterium. In this study, the effects of NK3-4 on rice growth and gene transcription were determined. The results indicated that a seed soaking treatment and a pre-germination seed treatment using NK3-4 promoted rice growth, especially spraying rice seedlings with NK3-4 increased the root number and root length by 34.2% and 34.1%, respectively. Moreover, NK3-4 induced the differential transcription of genes annotated with GO terms; the number of up-regulated genes was 4.38-times higher than the number of down-regulated genes. The NK3-4 treatment induced the differential transcription of genes in 1,794 GO functional groups, with 1,531 functional groups containing up-regulated genes. Specific growth-related genes up-regulated by NK3-4 are involved in biological processes, including responses to auxin, hormone biosynthesis, cellular component biogenesis, root system development and other functions. Furthermore, stress resistance-related genes were up-regulated, some of which encode WRKYs, NPK1-related protein kinase, NPR1-like 4, CaM-like proteins, MYBs, ERFs, TIFYs, NACs, EL5s, PR1s, PR2, PR8, PODs, PALs, PAD4. Considered together, these findings imply that NK3-4 may promote plant growth and enhance stress resistance by regulating gene expression, making it a potentially useful microbe for regulating rice growth and stress resistance.

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Expression Patterns and Functional Analysis of E3 Ubiquitin Ligase Genes in Rice

Cited by 2 publications

References 51 publications

Molecular Characterization of U-box E3 Ubiquitin Ligases (TaPUB2 and TaPUB3) Involved in the Positive Regulation of Drought Stress Response in Arabidopsis

Molecular Characterization of U-box E3 Ubiquitin Ligases (TaPUB2 and TaPUB3) Involved in the Positive Regulation of Drought Stress Response in Arabidopsis

Paenibacillus terrae NK3-4 regulates the transcription of growth-related and stress resistance-related genes in rice

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