Expression patterns and correlation analyses of muscle-specific genes in the process of sheep myoblast differentiation

Wang, Hejie; Dou, Min-Min; Li, Jun; Cao, Pan; Li, Junling; Guo, Tianyan; Zhao, Dipeng; Khan, Ajab; Li, Yingliang; Li, Baojun; Qin, Jian; Du, Rong

doi:10.1007/s11626-022-00721-7

Cited by 4 publications

(1 citation statement)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Two DAPs, namely, MYOZ2 and MYOM3, were identified in the comparison of three groups of experimental sheep. MYOZ2 belongs to the myozenin family, which binds calcineurin [ 26 ]. Calcineurin is a calcium/calmodulin-dependent serine, threonine phosphatase that plays an important role in transducing calcium-dependent signals in a variety of cell types [ 27 ].…”

Section: Discussionmentioning

confidence: 99%

DIA-Based Proteomic Analysis Reveals MYOZ2 as a Key Protein Affecting Muscle Growth and Development in Hybrid Sheep

Zhang,

Yue,

Yuan

et al. 2024

IJMS

View full text Add to dashboard Cite

Hybridization of livestock can be used to improve varieties, and different hybrid combinations produce unique breeding effects. In this study, male Southdown and Suffolk sheep were selected to hybridize with female Hu sheep to explore the effects of male parentage on muscle growth and the development of offspring. Using data-independent acquisition technology, we identified 119, 187, and 26 differentially abundant proteins (DAPs) between Hu × Hu (HH) versus Southdown × Hu (NH), HH versus Suffolk × Hu (SH), and NH versus SH crosses. Two DAPs, MYOZ2 and MYOM3, were common to the three hybrid groups and were mainly enriched in muscle growth and development-related pathways. At the myoblast proliferation stage, MYOZ2 expression decreased cell viability and inhibited proliferation. At the myoblast differentiation stage, MYOZ2 expression promoted myoblast fusion and enhanced the level of cell fusion. These findings provide new insights into the key proteins and metabolic pathways involved in the effect of male parentage on muscle growth and the development of hybrid offspring in sheep.

show abstract

Section: Discussionmentioning

confidence: 99%