Expression pattern of two zebrafish genes, cxcr4a and cxcr4b

Chong, Shang Wei; Emelyanov, Alexander; Gong, Zhiyuan; Korzh, Vladimir

doi:10.1016/s0925-4773(01)00520-2

Cited by 106 publications

(113 citation statements)

References 8 publications

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“…Finally, we observed that the sox3-expressing p4 subdomain migrates posteriorly from its original domain along the body axis after the 20-somite stage (data not shown). Given that this migration trend of the p4 subdomain is similar to that of the posterior lateral line primordium, as revealed by morphological observation (Laguerre et al, 2005) and expression of the chemokine receptor cxcr4 (Chong et al, 2001;David et al, 2002), we suggest that the p4 subdomain represents the anlage of the posterior lateral line system.…”

Section: Early Development Of the Epibranchial Placode In Zebrafishsupporting

confidence: 56%

Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal

Nikaido

Doi

Shimizu³

et al. 2006

Developmental Dynamics

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In vertebrates, cranial sensory ganglia are mainly derived from ectodermal placodes, which are focal thickenings at characteristic positions in the embryonic head. Here, we provide the first description of the early development of the epibranchial placode in zebrafish embryos using sox3 as a molecular marker. By the one-somite stage, we saw a pair of single sox3-expressing domains appear lateral to the future hindbrain. The sox3 domain, which is referred to here as the early lateral placode, is segregated during the early phase of segmentation to form a pax2a-positive medial area and a pax2a-negative lateral area. The medial area subsequently developed to form the otic placode, while the lateral area was further segregated along the anteroposterior axis, giving rise to four sox3-positive subdomains by 26 hr postfertilization. Given their spatial relationship with the expression of the markers for the epibranchial ganglion, as well as their positions and temporal changes, we propose that these four domains correspond to the facial, glossopharyngeal, vagal, and posterior lateral line placodes in an anterior-to-posterior order. The expression of sox3 in the early lateral placode was absent in mutants lacking functional fgf8, while implantation of fibroblast growth factor (FGF) beads restored the sox3 expression. Using SU5402, which inhibits the FGF signal, we were able to demonstrate that formation of both the early lateral domains and later epibranchial placodes depends on the FGF signal operating at the beginning of somitogenesis. Together, these data provide evidence for the essential role of FGF signals in the development of the epibranchial placodes. Developmental Dynamics 236:564 -571, 2007.

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Section: Early Development Of the Epibranchial Placode In Zebrafishsupporting

confidence: 56%

Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal

Nikaido

Doi

Shimizu³

et al. 2006

Developmental Dynamics

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“…Regardless of the precise nature and extent of the differences between both teleost IL-11 genes, apparently, there has been an evolutionary advantage to the possession of duplicate IL-11 genes in fish. As IL-11 is a pleiotropic cytokine, it is possible that the many functions it fulfills in mammals have been divided between two genes in bony fish, as has been suggested for other duplicate genes in fish (Chong et al 2001;Huising et al 2004b).…”

Section: Discussionmentioning

confidence: 99%

Multiple and highly divergent IL-11 genes in teleost fish

et al. 2005

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Interleukin-11 (IL-11) is a key cytokine in the regulation of proliferation and differentiation of hematopoietic progenitors and is also involved in bone formation, adipogenesis, and protection of mucosal epithelia. Despite this prominent role in diverse physiological processes, IL-11 has been described in only four mammalian species, and recently, in rainbow trout (Oncorhynchus mykiss). Here we report the presence of IL-11 in common carp (Cyprinus carpio), a bony fish species related to zebrafish. IL-11 is expressed in most carp organs and tissues. In vitro expression of IL-11 in cultured macrophages is enhanced by stimulation with lipopolysaccharide and is markedly inhibited by cortisol. A detailed and systematic scan of several fish genome databases confirms that IL-11 is present in all fish, but also reveals the presence of a second, substantially different IL-11 gene in the genomes of phylogenetically distant fish species. We designated both fish paralogues IL-11a and IL-11b. Although sequence identity between fish IL-11a and IL-11b peptides is low, the conservation of their gene structures supplemented by phylogenetic analyses clearly illustrate the orthology of both IL-11a and IL-11b genes of fish with mammalian IL-11. The presence of IL-11 genes in fish demonstrates its importance throughout vertebrate evolution, although the presence of duplicate and divergent IL-11 genes differs from the single IL-11 gene that exists in mammals.

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“…Cxcr4b is a chemokine receptor of the G-Protein Coupled Receptor (GPCR) gene family (Chong et al, 2001(Chong et al, , 2007). These receptors play an important role in defining directionality of cell migration of germ cells, lateral line, and somites during embryogenesis (Doitsidou et al, 2002;Gilmour et al, 2004;Hollway et al, 2007).…”

Section: Diffusion Coefficient Measurements In Live Zebrafish Embryosmentioning

confidence: 99%

“…A stop codon was removed from Cxcr4b gene previously cloned in a pPCR-Script vector (Stratagene, CA) (Chong et al, 2001). The Cxcr4b gene was digested out using SacII and XhoI and then subcloned into pEGFP-N1 (Clontech) vector digested with the same enzymes.…”

Section: Plasmidsmentioning

confidence: 99%

Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy

Shi

Teo

Pan

et al. 2009

Developmental Dynamics

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Zebrafish and Drosophila are animal models widely used in developmental biology. High-resolution microscopy and live imaging techniques have allowed the investigation of biological processes down to the cellular level in these models. Here, using fluorescence correlation spectroscopy (FCS), we show that even processes on a molecular level can be studied in these embryos. The two animal models provide different advantages and challenges. We first characterize their autofluorescence pattern and determine usable penetration depth for FCS especially in the case of zebrafish, where tissue thickness is an issue. Next, the applicability of FCS to study molecular processes is shown by the determination of blood flow velocities with high spatial resolution and the determination of diffusion coefficients of cytosolic and membrane-bound enhanced green fluorescent protein-labeled proteins in different cell types. This work provides an approach to study molecular processes in vivo and opens up the possibility to relate these molecular processes to developmental biology questions. Developmental Dynamics 238:3156-3167,

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Expression pattern of two zebrafish genes, cxcr4a and cxcr4b

Cited by 106 publications

References 8 publications

Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal

Initial specification of the epibranchial placode in zebrafish embryos depends on the fibroblast growth factor signal

Multiple and highly divergent IL-11 genes in teleost fish

Probing events with single molecule sensitivity in zebrafish and Drosophila embryos by fluorescence correlation spectroscopy

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