Expression Pattern of Sulfated Glycoprotein-2 (SGP-2) mRNA in Rat Testes Exposed to Endocrine Disruptors

Abstract

Abstract. Sulfated glycoprotein-2 (SGP-2)is secreted in Sertoli cells and epididymal epithelial cells and plays important roles in the regulation of spermatogenesis and sperm maturation. To investigate whether endocrine disruptors affect spermatogenesis through an SGP-2-dependent mechanism, daily oral doses of testosterone (50, 200 and 1,000 µg/kg), flutamide (1, 5 and 25 mg/kg), ketoconazole (0.2, 1, 5 and 25 mg/kg), diethylhexylphthalate (10, 50 and 250 mg/kg), nonylphenol (10, 50, 100 and 250 mg/kg), octylphenol (10, 50 and 250 mg/kg), diethylstilbesterol (10, 20 and 40 µg/kg) or corn oil (control) were administered to 5 week-old, male Sprague-Dawley rats for 3 weeks. Following treatment with these endocrine disruptors, testicular expression of SGP-2 mRNA was analyzed using reverse transcription-polymerase chain reaction. Compared with the control, the lowest dose of testosterone (50 µg/kg/day) significantly increased expression of SGP-2 mRNA, whereas 200 and 1,000 µg/kg/day testosterone significantly decreased the expression (P<0.05). Flutamide, ketoconazole, diethylhexylphthalate, nonylphenol, octylphenol and diethylstilbesterol significantly decreased SGP-2 mRNA expression in testes at all doses studied, with the exception of 1 mg/kg/day flutamide (P<0.05). These results suggest that endocrine disruptors might decrease spermatogenesis in testes by decreasing expression of SGP-2 mRNA.

The role of molecular chaperones in spermatogenesis and the post-testicular maturation of mammalian spermatozoa

“…CLU seems to play a role in initially protecting spermatogenic cells from heat damage and then later clearing damaged cells from the tubules. (Onoda and Djakiew 1990;Morales and Griswold 1991;Roberts et al 1991;Wong et al 1994;Ahuja et al 1996;Clark et al 1997;McKinnell and Sharpe 1997;Bailey et al 2002;Andersen et al 2003;Plotton et al 2005;Plotton et al 2006;Yon et al 2007)…”

“…CLU seems to play a role in initially protecting spermatogenic cells from heat damage and then later clearing damaged cells from the tubules. (Onoda and Djakiew 1990;Morales and Griswold 1991;Roberts et al 1991;Wong et al 1994;Ahuja et al 1996;Clark et al 1997;McKinnell and Sharpe 1997;Bailey et al 2002;Andersen et al 2003;Plotton et al 2005;Plotton et al 2006;Yon et al 2007) HSPD1 8 Mouse: Localized to the spermatogonia, primary (Meinhardt et al 1995;Paranko (HSP60) spermatocytes and Sertoli cells and then lost from more mature spermatogenic cells. Human: Localized to spermatogonia, primary spermatocytes and Sertoli cells.…”

Sperm mRNA Transcripts Are Indicators of Sub-Chronic Low Dose Testicular Injury in the Fischer 344 Rat

“…CLU is a glycoprotein involved in many biological processes, including protecting cells from injury, mediating apoptosis, and influencing the differentiation and maturation of germ cells [41]. Clu mRNA has previously been detected by qRT-PCR in porcine spermatozoa, and sperm are hypothesized to deliver the Clu mRNA transcript to the oocyte to support its subsequent development after fertilization [42], [43].…”