Expression pattern of oxygen and stress-responsive gene transcripts at various developmental stages of in vitro and in vivo preimplantation bovine embryos

Balasubramanian, S.; Son, Woo-Jin; Kumar, B. Mohana; Ock, Sun‐A; Yoo, Jae Gyu; Im, Gi‐Sun; Choe, Sang-Yong; Rho, Gyu‐Jin

doi:10.1016/j.theriogenology.2007.05.044

Cited by 70 publications

(67 citation statements)

References 54 publications

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“…MnSOD protects cells against oxidative damage [45] and iNOS has a role in germinal vesicle breakdown and the anaphase-telophase transition of oocytes [46], therefore, its upregulation may negatively impact buffalo oocytes during maturation and subsequent embryo development. Our results are in accordance with the study by Balasubramanium et al [47] where the expression of MnSOD decreased when embryos were cultured under high oxygen concentration (20 %) compared to the 5 % oxygen concentration normally occurring in vivo. Since MnSOD is a mitochondrial enzyme [45][46][47], the decrease in its expression may be related to the altered mitochondrial activity after subjecting the oocytes to heat stress thereby, negatively impacting the developmental competence.…”

Section: Discussionsupporting

confidence: 93%

“…Our results are in accordance with the study by Balasubramanium et al [47] where the expression of MnSOD decreased when embryos were cultured under high oxygen concentration (20 %) compared to the 5 % oxygen concentration normally occurring in vivo. Since MnSOD is a mitochondrial enzyme [45][46][47], the decrease in its expression may be related to the altered mitochondrial activity after subjecting the oocytes to heat stress thereby, negatively impacting the developmental competence. However, the exact mechanism of down regulation remains unclear and warrants further validation.…”

Section: Discussionsupporting

confidence: 93%

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Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Ashraf

Shah

Saini

et al. 2014

J Assist Reprod Genet

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Objective To determine the direct effect of physiologically relevant high temperatures (40.5 and 41.5°C) for two time periods (12 and 24 h) on bubaline oocytes during in vitro maturation. Method The control group oocytes were cultured at 38.5°C for 24 h. The treatment 1 (T1) and 3 (T3) group oocytes were cultured at 40.5 and 41.5°C respectively, for the first 12 h and at 38.5°C for rest of the 12 h. However, treatment 2 (T2) and 4 (T4) group oocytes were cultured at 40.5 and 41.5°C for complete 24 h. Results Development of oocytes to blastocyst was severely compromised (p<0.001) when matured at 40.5 and 41.5°C for both exposure periods (12 h and 24 h). It was found that the cleavage rates, blastocyst yield and mean cell number decreased remarkably (p<0.001) in the treatment groups compared to control. The relative mRNA expression of heat shock protein (Hsp 70.1, 70.2, 70.8, 60, 10 and HSF1), pro-apoptotic (caspases-3, −7, −8, Bid and Bax) and oxidative stress (iNOS) related genes was significantly higher (p<0.05) in all the treatment groups compared to control. However, mRNA abundance of anti-apoptotic (Bcl-2, Mcl-1, Bcl-xl), glucose transport (Glut1, Glut3 and IGF1R), developmental competence (ZAR1 and BMP15) and oxidative stress (MnSOD) related genes was significantly decreased (p<0.05) in the treatment groups compared to control. Conclusion The present study clearly establishes that physiologically relevant elevated temperatures during in vitro meiotic maturation reduce developmental competence of bubaline oocytes.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionsupporting

confidence: 93%

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Ashraf

Shah

Saini

et al. 2014

J Assist Reprod Genet

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“…Expression of SLC2A1 was reduced in parthenotes, while SLC2A5 (a fructose transporter) did not change. Bovine in vivo derived embryos express SLC2A1 and SLC2A5 (Augustin et al 2001, Balasubramanian et al 2007. However, IVP embryos show reduced SLC2A1 mRNA abundance as compared with in vivo counterparts (Lazzari et al 2002, Balasubramanian et al 2007).…”

Section: Development and Gene Expression In Parthenotesmentioning

confidence: 99%

“…Bovine in vivo derived embryos express SLC2A1 and SLC2A5 (Augustin et al 2001, Balasubramanian et al 2007. However, IVP embryos show reduced SLC2A1 mRNA abundance as compared with in vivo counterparts (Lazzari et al 2002, Balasubramanian et al 2007). The expression of SLC2A1 in IVP embryos has been found in the ICM and the TE (Wrenzycki et al 2003).…”

Section: Development and Gene Expression In Parthenotesmentioning

confidence: 99%

Biological differences between in vitro produced bovine embryos and parthenotes

Gómez¹,

Gutiérrez‐Adán²,

Díez³

et al. 2009

Reproduction

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Parthenotes may represent an alternate ethical source of stem cells, once biological differences between parthenotes and embryos can be understood. In this study, we analyzed development, trophectoderm (TE) differentiation, apoptosis/necrosis, and ploidy in parthenotes and in vitro produced bovine embryos. Subsequently, using real-time PCR, we analyzed the expression of genes expected to underlie the observed differences at the blastocyst stage. In vitro matured oocytes were either fertilized or activated with ionomycin C6-DMAP and cultured in simple medium. Parthenotes showed enhanced blastocyst development and diploidy and reduced TE cell counts. Apoptotic and necrotic indexes did not vary, but parthenotes evidenced a higher relative proportion of apoptotic cells between inner cell mass and TE. The pluripotence-related POU5F1 and the methylation DNMT3A genes were downregulated in parthenotes. Among pregnancy recognition genes, TP-1 was upregulated in parthenotes, while PGRMC1 and PLAC8 did not change. Expression of p66 shc and BAX/BCL2 ratio were higher, and p53 lower, in parthenotes. Among metabolism genes, SLC2A1 was downregulated, while AKR1B1, PTGS2, H6PD, and TXN were upregulated in parthenotes, and SLC2A5 did not differ. Among genes involved in compaction/blastulation, GJA1 was downregulated in parthenotes, but no differences were detected within ATP1A1 and CDH1. Within parthenotes, the expression levels of SLC2A1, TP-1, and H6PD, and possibly AKR1B1, resemble patterns described in female embryos. The pro-apoptotic profile is more pronounced in parthenotes than in embryos, which may differ in their way to channel apoptotic stimuli, through p66 shc and p53respectively, and in their mechanisms to control pluripotency and de novo methylation.Reproduction (2009) 137 285-295

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“…So, in our study we selected the two genes (Na/K-ATPase, Glut-1) which are very closely related to blastocyst expansion and hatching (Wrenzycki et al, 2003). And Glut-1 is also an important gene related to the transport and metabolism of glucose and the very high expression levels of Glut-1 may be associated with high quality embryos (Rizos et al, 2002;Wrenzycki et al, 2003;Balasubramanian et al, 2007). We found that the expression levels of Na/K-ATPase and Glut-1 obtained in M2 media were significantly higher than those obtained in M1 media.…”

Section: Discussionmentioning

confidence: 99%

Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro

Shao¹,

Lan²,

Liu³

et al. 2010

CZECH J ANIM SCI

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ABSTRACT:To improve the culture system of bovine somatic cell nuclear transfer (SCNT) embryos, we studied the effects of activin A on developmental competence of bovine SCNT embryos during the early development stage based on the traditional culture method, and analyzed the expression level of the genes related to blastocyst hatching (Na/K-ATPase, Glut-1) and related to activin A signalling pathway (ActRII and Smad2). We generated the bovine SCNT embryo using a Holstein cow oocyte as recipient cytoplasm and a foetal ear fibroblast (Holstein cow, 120 days) as donor cell. The embryos were cultured as follows: experiment 1, the addition of activin A at the concentrations of 0 (control), 20 (M1-20), 40 (M1-40) or 80 ng/ml (M1-80) to the media during the first three days and no addition during the subsequent 5 days; experiment 2, no addition of activin A to the media during the first 3 days and the addition of activin A at the concentrations of 0 (control), 20 (M2-20), 40 (M2-40) or 80 ng/ml (M2-80) during the subsequent 5 days. The results indicated that the blastocyst formation rate and hatching rate, and total blastomere numbers as well as ICM/TE obtained in experiment 1 were not significantly different from the control group (P > 0.05). In contrast, these values obtained in experiment 2 were significantly higher than in the control group (P < 0.05). In addition, the relative abundance (ratio to GAPDH mRNA) of each gene (Glut-1, ActR II and Smad2) was not significantly different among the treatments in the experiment. The expression levels of 4 genes (Na/K-ATPase, Glut-1, ActR II and Smad2) in blastocysts obtained in experiment 2 were higher than those obtained in experiment 1. In conclusion, the present study suggests that the addition of activin A to the culture media from day 4 to day 8 can enhance the developmental competence of bovine SCNT embryos.

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Expression pattern of oxygen and stress-responsive gene transcripts at various developmental stages of in vitro and in vivo preimplantation bovine embryos

Cited by 70 publications

References 54 publications

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Developmental competence and expression pattern of bubaline (Bubalus bubalis) oocytes subjected to elevated temperatures during meiotic maturation in vitro

Biological differences between in vitro produced bovine embryos and parthenotes

Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro

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