Expression pattern of matrix metalloproteinases changes during folliculogenesis in the cat ovary

Abstract

Matrix metalloproteinase (MMP) has been implicated as having roles in ovarian folliculogenesis. Here, we determined the expression pattern of six MMPs (MMP1, MMP2, MMP3, MMP7, MMP9 and MMP13) and their endogenous tissue inhibitor, TIMP1, during cat follicle growth. Different developmental stage follicles were mechanically isolated and gene expression analysed by real-time qPCR while MMP1, 2, 9 and 13 localization was determined by immunohistochemistry. With the exception of MMP13, the amount of MMP mRNA was lowest in primordial follicles and increased thereafter. Peak levels were detected in early antral follicles for MMP1 (72.2-fold increase above primordial follicle amount), MMP2 (10-fold), MMP3 (57-fold) and MMP9 (2.8-fold). MMP7 transcripts increased 2-fold by the primary follicle stage and then plateaued. MMP13 mRNA peaked in primary follicles (2.5-fold) and was lower in more advanced counterparts. TIMP1 sharply increased (6-fold) in secondary follicles and gradually declined in the later stages. MMP1 and MMP9 expression were expressed in the granulosa cells of all follicle stages. MMP2 was immunoreactive in early and antral follicles, especially at granulosa cells adjacent to the antral cavity. By contrast, the MMP13 was weakly detected in primary follicles onward. In summary, there are distinctive and consistent changes in MMPs and TIMP1 expression during follicle development, suggesting that these enzymes play one or more roles in cat folliculogenesis. In particular, high mRNA and protein expression levels of MMP1 and MMP2, especially at the antral stage, indicate that these enzymes likely are involved in antrum formation and expansion.

Telomerase Reverse Transcriptase (TERT) Expression, Telomerase Activity, and Expression of Matrix Metalloproteinases (MMP)-1/-2/-9 in Feline Oral Squamous Cell Carcinoma Cell Lines Associated With Felis catus Papillomavirus Type-2 Infection

“…The following primers for feline cMyc were designed based on the gene sequence previously published: cMyc-FW: 5 ′ -CAAAAGGTCGGAATCGGGGT-3 ′ , cMyc-REV: 5 ′ -CGTGGCATCTCTTAAGGACCA-3 ′ (33). Feline MMP-1, MMP-2, and MMP-9 genes were amplified by using the primers described previously (34)(35)(36). Amplification of feline β-2-microglobulin (β2MG) was performed in parallel to allow normalization of the results as previously reported (37).…”

ID3 mediates the TGF‐β1‐induced suppression of matrix metalloproteinase‐1 in human granulosa cells

“…The MMP family belongs to zinc‐ and calcium‐dependent metalloproteinases and encompasses at least 28 members that can be subdivided into four broad classes: collagenases, gelatinases, stromelysins, and membrane‐type MMPs . Several members of the MMP family (e.g., MMP1, MMP2, MMP7, MMP9, and MMP13) are expressed in mammalian ovarian follicles at different developmental stages, and the expression of these MMPs is highly regulated in response to gonadotropins, sex hormones, and growth factors . For instance, MMP1 and MMP9 are expressed in granulosa cells at all follicular stages, and MMP2 is expressed in the granulosa/cumulus cells of early and antral follicles .…”

“…Several members of the MMP family (e.g., MMP1, MMP2, MMP7, MMP9, and MMP13) are expressed in mammalian ovarian follicles at different developmental stages, and the expression of these MMPs is highly regulated in response to gonadotropins, sex hormones, and growth factors . For instance, MMP1 and MMP9 are expressed in granulosa cells at all follicular stages, and MMP2 is expressed in the granulosa/cumulus cells of early and antral follicles . A recent study performed using clinical samples showed that MMP2 and MMP9 are present in follicular fluid and that MMP2 activity is positively correlated with oocyte and embryo quality .…”

Comprehensive Analysis of miRNA-mRNA/lncRNA During Gonadal Development of Triploid Rainbow Trout (Oncorhynchus mykiss)

“…In this study, the biological analysis showed that the differentially expressed genes (DEGs) which were enriched in the PPAR signalling pathway included matrix metalloproteinase 13 (MMP13), which upregulated at 65 dpf and 180 dpf, but downregulated at 600 dpf. MMP13 is mainly expressed in the primary follicles and plays an important role in follicle development along with MMP1 (Fujihara et al, 2016). The study found that PLIN1 was speci cally expressed in the testis and its mRNA level decreased signi cantly with development, which was con rmed to play an important role in the early stage of the rst meiosis of spermatogenesis (Chen et al, 2014).…”