Expression pattern of human SERPINE2 in a variety of human tumors

Yang, Ying; Xin, Xiangke; Fu, Xing; Xu, Danmei

doi:10.3892/ol.2018.7819

Cited by 20 publications

(23 citation statements)

References 42 publications

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“…Of the significantly up-regulated genes for Pt224 (553 genes) and Pt412 (507 genes), a total of 142 genes were commonly upregulated in both patient samples. From these 142 genes, we identified and binned 9 of the most highly upregulated genes associated with stemness ( WLS [39], [40], ALDH1A1 [41], [42], BMP2 [38], [43], RSPO3 [44], [45]), tumorigenicity ( MAGEB2 [46], [47], BMP2 [38], EFNB2 [48], SERPINE2 [49], [50], [51], [52], [53], HHIP [54], [55], [56], PTGS2 [57], [58], [59], [60]), and chemoresistance ( WLS [39], [40], [61], ALDH1A1 [62], [63], BMP2 [38], PTGS2 [57], [64]) based on the literature (Figure 7, C , D ). The upregulation of these genes between P0 and P6 for both patient samples was subsequently confirmed with qRT-PCR.…”

Section: Resultsmentioning

confidence: 99%

Engineered 3D Model of Cancer Stem Cell Enrichment and Chemoresistance

et al. 2019

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Intraperitoneal dissemination of ovarian cancers is preceded by the development of chemoresistant tumors with malignant ascites. Despite the high levels of chemoresistance and relapse observed in ovarian cancers, there are no in vitro models to understand the development of chemoresistance in situ . Method: We describe a highly integrated approach to establish an in vitro model of chemoresistance and stemness in ovarian cancer, using the 3D hanging drop spheroid platform. The model was established by serially passaging non-adherent spheroids. At each passage, the effectiveness of the model was evaluated via measures of proliferation, response to treatment with cisplatin and a novel ALDH1A inhibitor. Concomitantly, the expression and tumor initiating capacity of cancer stem-like cells (CSCs) was analyzed. RNA-seq was used to establish gene signatures associated with the evolution of tumorigenicity, and chemoresistance. Lastly, a mathematical model was developed to predict the emergence of CSCs during serial passaging of ovarian cancer spheroids. Results: Our serial passage model demonstrated increased cellular proliferation, enriched CSCs, and emergence of a platinum resistant phenotype. In vivo tumor xenograft assays indicated that later passage spheroids were significantly more tumorigenic with higher CSCs, compared to early passage spheroids. RNA-seq revealed several gene signatures supporting the emergence of CSCs, chemoresistance, and malignant phenotypes, with links to poor clinical prognosis. Our mathematical model predicted the emergence of CSC populations within serially passaged spheroids, concurring with experimentally observed data. Conclusion: Our integrated approach illustrates the utility of the serial passage spheroid model for examining the emergence and development of chemoresistance in ovarian cancer in a controllable and reproducible format.

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Section: Resultsmentioning

confidence: 99%

Engineered 3D Model of Cancer Stem Cell Enrichment and Chemoresistance

et al. 2019

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“…For instance, down-regulation in the expression of AGER is associated with modulation of cell apoptosis, localization of NF-κB, phosphorylation of ERK1/2, and astrocyte reactivity leading toward neurodegeneration (Wang et al, 2002; Ohtaki et al, 2007; Choi et al, 2014; Kim et al, 2015; Gu et al, 2016). Differential expression of other molecules like MMP-2 (Matrix Matalloproteinase-2) and SERPINES 1/2 (Serpin family E member 1 and 2) results in the proteolytic cleavage of pericellular proteins and breakdown of extra-cellular matrix, stalls cell migration and neurite remodeling in the brain, which in turn can impact brain development (Ogier et al, 2006; Hagemann et al, 2012; Kamat et al, 2014; Wu et al, 2018; Yang et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Zika Virus Infection Disrupts Astrocytic Proteins Involved in Synapse Control and Axon Guidance

2019

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The first human Zika virus (ZIKV) outbreak was reported in Micronesia in 2007, followed by one in Brazil in 2015. Recent studies have reported cases in Europe, Oceania and Latin America. In 2016, ZIKV transmission was also reported in the US and the World Health Organization declared it a Public Health Emergency of International Concern. Because various neurological conditions are associated with ZIKV, such as microcephaly, Guillain-Barré syndrome, and other disorders of both the central and peripheral nervous systems, including encephalopathy, (meningo)encephalitis and myelitis, and because of the lack of reliable patient diagnosis, numerous ongoing studies seek to understand molecular mechanisms underlying ZIKV pathogenesis. Astrocytes are one of the most abundant cells in the CNS. They control axonal guidance, synaptic signaling, neurotransmitter trafficking and maintenance of neurons, and are targeted by ZIKV. In this study, we used a newly developed multiplexed aptamer-based technique (SOMAScan) to examine > 1300 human astrocyte cell proteins. We identified almost 300 astrocyte proteins significantly dysregulated by ZIKV infection that span diverse functions and signaling pathways, including protein translation, synaptic control, cell migration and differentiation.

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“…In silico analysis revealed that SERPINE2 theoretically could form a fusion gene with the EMCN at 4q23 ( Figure 4 ). The SERPINE2 gene, expressing a serine proteinase inhibitor clade E member 2, was recently shown to be a potential biomarker for tumor diagnosis and prognosis in a variety of solid tumors [ 42 ]. Although the EMCN gene previously has been shown to be overexpressed in acute leukemia [ 43 ] further studies are needed to clarify the potential role, if any, of the SERPINE2 and EMCN genes in the pathogenesis of the present patient.…”

Section: Discussionmentioning

confidence: 99%

A Novel Acquired t(2;4)(q36.1;q24) with a Concurrent Submicroscopic del(4)(q23q24) in An Adult with Polycythemia Vera

Kjeldsen

2018

Cancers

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Background: Polycythemia vera (PV) is a clonal myeloid stem cell disease characterized by a growth-factor independent erythroid proliferation with an inherent tendency to transform into overt acute myeloid malignancy. Approximately 95% of the PV patients harbor the JAK2V617F mutation while less than 35% of the patients harbor cytogenetic abnormalities at the time of diagnosis. Methods and Results: Here we present a JAK2V617F positive PV patient where G-banding revealed an apparently balanced t(2;4)(q35;q21), which was confirmed by 24-color karyotyping. Oligonucleotide array-based Comparative Genomic Hybridization (aCGH) analysis revealed an interstitial 5.4 Mb large deletion at 4q23q24. Locus-specific fluorescent in situ hybridization (FISH) analyses confirmed the mono-allelic 4q deletion and that it was located on der(4)t(2;4). Additional locus-specific bacterial artificial chromosome (BAC) probes and mBanding refined the breakpoint on chromosome 2. With these methods the karyotype was revised to 46,XX,t(2;4)(q36.1;q24)[18]/46,XX[7]. Conclusions: This is the first report on a PV patient associated with an acquired novel t(2;4)(q36.1;q24) and a concurrent submicroscopic deletion del(4)(q23q24). The study also underscores the benefit of combined usage of FISH and oligo-based aCGH analysis in characterizing chromosomal abnormalities. The present findings provide additional clues to unravel important molecular pathways in PV to obtain the full spectrum of acquired chromosomal and genomic aberrations, which eventually may improve treatment options.

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Expression pattern of human SERPINE2 in a variety of human tumors

Cited by 20 publications

References 42 publications

Engineered 3D Model of Cancer Stem Cell Enrichment and Chemoresistance

Engineered 3D Model of Cancer Stem Cell Enrichment and Chemoresistance

Zika Virus Infection Disrupts Astrocytic Proteins Involved in Synapse Control and Axon Guidance

A Novel Acquired t(2;4)(q36.1;q24) with a Concurrent Submicroscopic del(4)(q23q24) in An Adult with Polycythemia Vera

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