Expression pattern analysis of IRF4 and its related genes revealed the functional differentiation of IRF4 paralogues in teleost

Ai, Kelong; Luo, Kai; Li, Youshen; Hu, Wei; Gao, Weihua; Liu, Fang; Tian, Guangming; Ruan, Guoliang; Xu, Qiaoqing

doi:10.1016/j.fsi.2016.11.038

Cited by 29 publications

(16 citation statements)

References 36 publications

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“…In the present study, constitutive expression of CcIRF10 gene was detectable in all the eleven tissues of C. carpio when analysed by Real-time PCR, despite differences at the level of expression. This ubiquitous tissue expression pattern supported previous studies of IRFs in teleost, including M. albus [17], G. morhua [13], D. rerio [34], O. mykiss [35], turbot (Scophthalmus maximus) [36], P. olivaceus [12,37], rock bream (Oplegnathus fasciatu) [38], blunt snout bream (Megalobrama amblycephala) [39] and half-smooth tongue sole (Cynoglossus semilaevis) [40]. CcIRF10 was found to be most highly expressed in gonad (Fig.…”

Section: Discussionsupporting

confidence: 88%

Identification and Functional Analysis of A Non-Mammalian IRF10 in Common Carp (Cyprinus Carpio. L) in The Antiviral and Antibacterial Immune Response

Zhu

Shan

Zhao

et al. 2020

Preprint

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Background: Interferon (IFN) regulatory factors (IRFs), as the transcriptional regulatory factors play important roles in regulating the expression of type I IFN and IFN-stimulated genes (ISGs) in innate immune responses, as well as participate in cell growth, development and regulating the oncogenesis.Results: In the present study, the cDNA sequence of IRF10 was characterized in common carp (Cyprinus carpio L.) (abbreviation, CcIRF10). The predicted protein sequence of CcIRF10 shared 52.7-89.2% identities to teleost IRF10 and contained a DNA-binding domain (DBD), a nuclear localization signal (NLS) and an IRF associated domain (IAD). Phylogenetic analysis showed that CcIRF10 had a closer relationship with the IRF10 of Ctenopharyngodon idella. CcIRF10 transcripts was detectable in all examined tissues, with the highest expression in the gonad and the lowest expression level in the head kidney. The CcIRF10 expression was up-regulated in the spleen, head kidney, foregut and hindgut upon polyinosinic: polycytidylic acid (poly I:C) and Aeromonas hydrophila stimulation, and also induced by poly I:C, LPS and PGN in the peripheral blood leucocytes (PBLs) and head kidney leukocytes (HKLs) of C. carpio. In addition, overexpression of CcIRF10 was able to decrease the expressions of IFN-related genes, PKR and ISG5.Conclusions: These results indicated that CcIRF10 participated in the antiviral and antibacterial immunity, and negatively regulated the IFN response, which provided new insights into the IFN system of C. carpio.

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Section: Discussionsupporting

confidence: 88%

Identification and Functional Analysis of A Non-Mammalian IRF10 in Common Carp (Cyprinus Carpio. L) in The Antiviral and Antibacterial Immune Response

Zhu

Shan

Zhao

et al. 2020

Preprint

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“… References Gene ID b Chr. References st8sia3* XIV (XIII) 74 ppar beta* XII 65 ahr1* Ι (XVI) 75 uts2a* XII 76 dag1* XVII (XII) 77 gli2* XVI 28 marcks* XVIII (XV) 78 mustn1b* XVII 79 ncam1* VII (I) 80 bmp2b* XVIII 81 stard13 VII (I) 82 cart1* XV 83 otx2* XV (XVIII) 84 zic2a* XVI 21 otx5* I (VII) 84 mgrn1b* XI 21 tshr XVIII (XIV) 85 hoxc6α* XII 31 nme2a* scf48 (Un) 86 hoxc11α* XII 31 irf4 VIII (III) 87 hoxc12α* XII …”

Section: Resultsmentioning

confidence: 99%

Unravelling paralogous gene expression dynamics during three-spined stickleback embryogenesis

Kaitetzidou

Katsiadaki

Lagnel

et al. 2019

Sci Rep

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Development requires the implementation of a plethora of molecular mechanisms, involving a large set of genes to ensure proper cell differentiation, morphogenesis of tissues and organs as well as the growth of the organism. Genome duplication and resulting paralogs are considered to provide the raw genetic materials important for new adaptation opportunities and boosting evolutionary innovation. The present study investigated paralogous genes, involved in three-spined stickleback ( Gasterosteus aculeatus ) development. Therefore, the transcriptomes of five early stages comprising developmental leaps were explored. Obtained expression profiles reflected the embryo’s needs at different stages. Early stages, such as the morula stage comprised transcripts mainly involved in energy requirements while later stages were mostly associated with GO terms relevant to organ development and morphogenesis. The generated transcriptome profiles were further explored for differential expression of known and new paralogous genes. Special attention was given to hox genes, with hoxa13a being of particular interest and to pigmentation genes where itgb1 , involved in the melanophore development, displayed a complementary expression pattern throughout studied stages. Knowledge obtained by untangling specific paralogous gene functions during development might not only significantly contribute to the understanding of teleost ontogenesis but might also shed light on paralogous gene evolution.

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“…Intrigued by these results, we next sought to identify whether the expression of any PPAR signaling components would situate them as possible candidates for involvement in nephron development. We surveyed online expression repositories and published literature and found that transcripts encoding ppargc1a / PGC-1α have been detected in nascent nephrons within the developing zebrafish pronephros and the mouse metanephros ( Bertrand et al, 2007 ; Thisse and Thisse, 2008 ; Diez-Roux et al, 2011 ; Ai et al, 2017 ; Finger et al, 2017 ). To further investigate this, we performed WISH on WT embryos and assessed the spatiotemporal patterns of ppargc1a expression throughout the stages of zebrafish pronephros ontogeny.…”

Section: Resultsmentioning

confidence: 99%

ppargc1a controls nephron segmentation during zebrafish embryonic kidney ontogeny

Chambers

Poureetezadi

Addiego

et al. 2018

eLife

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Nephron segmentation involves a concert of genetic and molecular signals that are not fully understood. Through a chemical screen, we discovered that alteration of peroxisome proliferator-activated receptor (PPAR) signaling disrupts nephron segmentation in the zebrafish embryonic kidney (Poureetezadi et al., 2016). Here, we show that the PPAR co-activator ppargc1a directs renal progenitor fate. ppargc1a mutants form a small distal late (DL) segment and an expanded proximal straight tubule (PST) segment. ppargc1a promotes DL fate by regulating the transcription factor tbx2b, and restricts expression of the transcription factor sim1a to inhibit PST fate. Interestingly, sim1a restricts ppargc1a expression to promote the PST, and PST development is fully restored in ppargc1a/sim1a-deficient embryos, suggesting Ppargc1a and Sim1a counterbalance each other in an antagonistic fashion to delineate the PST segment boundary during nephrogenesis. Taken together, our data reveal new roles for Ppargc1a during development, which have implications for understanding renal birth defects.

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Expression pattern analysis of IRF4 and its related genes revealed the functional differentiation of IRF4 paralogues in teleost

Cited by 29 publications

References 36 publications

Identification and Functional Analysis of A Non-Mammalian IRF10 in Common Carp (Cyprinus Carpio. L) in The Antiviral and Antibacterial Immune Response

Identification and Functional Analysis of A Non-Mammalian IRF10 in Common Carp (Cyprinus Carpio. L) in The Antiviral and Antibacterial Immune Response

Unravelling paralogous gene expression dynamics during three-spined stickleback embryogenesis

ppargc1a controls nephron segmentation during zebrafish embryonic kidney ontogeny

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