Expression of β-defensins in intestines of chickens injected with vitamin D3 and lipopolysaccharide

Lu, Lu; Li, S.M.; Zhang, L.; Liu, X.Q.; Li, D.Y.; Zhao, Xiaoling; Liu, Y.P.

doi:10.4238/2015.april.13.12

Cited by 15 publications

(19 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…These observations support the results of previous reports, showing that the AvBD genes were expressed in the small intestine and cecum of embryos and chicks (Akbari et al, 2008;Crhanova et al, 2011;Hong et al, 2012;Lu et al, 2015;Zang et al, 2016;Su et al, 2017;Terada et al, 2018). AvBD2 in the mucosal leukocytes (Terada et al, 2018) and AvBD9 in enteroendocrine cells from the luminal and crypt epithelia have also been reported (Cuperus et al, 2016).…”

Section: Discussionsupporting

confidence: 91%

Effects of Toll-like Receptor Ligands on the Expression of Proinflammatory Cytokines and Avian <i>β</i>-defensins in Cultured Chick Intestine

et al. 2020

View full text Add to dashboard Cite

Few studies have focused on the regulation of cytokine and avian β-defensin (AvBDs) expression for promoting immune defense in the avian intestine. The aim of this study was to investigate the effects of different Toll-like receptor (TLR) ligands (bacterial patterns) on the expression of proinflammatory cytokines (IL-1β and IL-6) and AvBDs (AvBD1, AvBD4, and AvBD7) in the chick intestine. The ileum and cecum of 3-day-old chicks were collected and examined histologically to identity the cells present in the intestinal mucosa. Other tissues were cultured with or without the TLR2, TLR4, and TLR21 ligands-Pam3CSK4, LPS, and CpG-ODN-for 1 or 3 h. The gene expression profiles of proinflammatory cytokines and AvBDs were determined in these tissues using real-time polymerase chain reaction (PCR). The mucosa of the ileum and cecum contained leukocytes, luminal and crypt epithelial cells, and other enterocytes. Pam3CSK4 tended to downregulate the expression of IL-1β, AvBD1, and AvBD7 in the ileum but upregulated their expression in the cecum. LPS downregulated the expression of IL-1β and IL-6 in both the ileum and the cecum, whereas it upregulated the expression of AvBD1, AvBD4, and AvBD7 in the cecum. CpG-ODN upregulated the expression of IL-6 and AvBD7 in the ileum and IL-1β in the cecum, and downregulated the expression of IL-1β and AvBDs in the ileum. We suggested that the expression levels of proinflammatory cytokines and AvBDs in the chick intestine are affected by TLR2, TLR4, and TLR21 ligands. Thus, these innate immune factors may be modulated by the luminal microbe complex in the intestine.

show abstract

Section: Discussionsupporting

confidence: 91%

Effects of Toll-like Receptor Ligands on the Expression of Proinflammatory Cytokines and Avian <i>β</i>-defensins in Cultured Chick Intestine

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Prior studies in our lab demonstrated that injection of Vit-D 3 is able to modulate AvBD gene expression in intestinal tissues [ 23 ]. To confirm the previous results, CEIEPCs were used as a model to evaluate the action of 1,25D 3 on the expression of AvBDs with or without LPS.…”

Section: Resultsmentioning

confidence: 99%

“…Due to the presence of potential VDREs in the promoters of chicken AvBDs [ 23 ], we hypothesized 1,25D 3 may increase the relative mRNA abundance of some AvBD genes through VDREs. In this experiment, CHX pretreatment completely inhibited 1,25D 3 -mediated up-regulation of three AvBD genes in PBMCs, however, CHX further increased the expression of CYP24A1 , the primary VDR/1,25D 3 target gene [ 28 ].…”

Section: Discussionmentioning

confidence: 99%

1,25-Dihydroxyvitamin-D3 Induces Avian β-Defensin Gene Expression in Chickens

Zhang

et al. 2016

PLoS ONE

View full text Add to dashboard Cite

Host defense peptides (HDPs) play a critical role in innate immunity. Specific modulation of endogenous HDP synthesis by dietary compounds has been regarded as a novel approach to boost immunity and disease resistance in animal production. 1,25-dihydroxy vitamin D3 (1,25D3) is well known as a powerful HDP inducer in humans, but limited information about the effect of 1,25D3 on HDPs in poultry is available. Here, we sought to examine whether 1,25D3 could stimulate avian β-defensin (AvBD) expression in chickens. We used chicken embryo intestinal epithelial cells (CEIEPCs) and peripheral blood mononuclear cells (PBMCs) to study the effect of 1,25D3 on the expression of AvBDs. We observed that 1,25D3 is able to up-regulate the expression of several AvBDs in CEIEPCs and PBMCs, whereas it increased the amounts of AvBD4 mRNA in CEIEPCs only in the presence of lipopolysaccharide (LPS). On the other hand, LPS treatment not only inhibited the expression of CYP24A1 but also altered the expression pattern of VDR in CEIEPCs. Furthermore, AvBDs were not directly regulated by 1,25D3, as cycloheximide completely blocked 1,25D3-induced expression of AvBDs. Our observations suggest that 1,25D3 is capable of inducing AvBD gene expression and is a potential antibiotic alternative through augmentation of host innate immunity as well as disease control in chickens.

show abstract

“…The innate immune response is the first line of defense against infection. The epithelial cells of the intestinal mucosa facilitate efficient host defense by producing the HDP genes [34], which plays a significant role in the chicken innate immune system, providing the first line of defense against potential pathogens [16,22,35,36]. We found that the Daweishan mini chickens showed the highest mRNA levels of HDP genes mRNA in small intestine tissue while Avian broilers exhibited the lowest mRNA levels of HDP genes.…”

Section: Hdp Genes In the Small Intestinementioning

confidence: 78%

Profiles of expression pattern and tissue distribution of host defense peptides genes in different chicken (Gallus gallus) breeds related to body weight

Ahmed

et al. 2020

Preprint

View full text Add to dashboard Cite

Host defense peptides (HDPs) are an important first line of defense with antimicrobial and immunomodulatory properties. Selection for increased body weight is suggested to be related to reduced immune response. We studied the relationships among body weight, age, and the HDP expression patterns in intestine and immune organs. We used chickens with marked differences of body sizes. The non-selected Daweishan mini chickens showed the highest indexes of immune organs and the lowest concentrations of the plasma immune parameters C3, C4, IgA, and IgY, while the commercial Avian broiler showed the opposite results. The Daweishan mini chickens showed the highest mRNA expressions of HDP genes in small intestine followed by the semi-selected Wuding chickens. Compared with local breeds, broiler chickens showed higher mRNA expression of HDP genes in spleen, thymus, and bursa. Body weight and HDP expression levels were negatively correlated in the intestine and positively in the immune organs. These results indicated that the HDP immune regulatory roles in small intestine acted as first line of defense in innate immunity in local breeds, and as an adaptive immunity in broiler chickens. Selection was associated with the expression profiles of HDP genes in a chicken, age, and organ-specific manner.

show abstract

Expression of β-defensins in intestines of chickens injected with vitamin D3 and lipopolysaccharide

Cited by 15 publications

References 30 publications

Effects of Toll-like Receptor Ligands on the Expression of Proinflammatory Cytokines and Avian <i>β</i>-defensins in Cultured Chick Intestine

Effects of Toll-like Receptor Ligands on the Expression of Proinflammatory Cytokines and Avian <i>β</i>-defensins in Cultured Chick Intestine

1,25-Dihydroxyvitamin-D3 Induces Avian β-Defensin Gene Expression in Chickens

Profiles of expression pattern and tissue distribution of host defense peptides genes in different chicken (Gallus gallus) breeds related to body weight

Contact Info

Product

Resources

About