Expression of β-Amyloid Precursor Protein in the Porcine Ovary

Kimura, Atsushi; Kakinuma, Kazumi; Yonezawa, Satoshi; Takahashi, Takayuki

doi:10.2108/zsj.17.769

Cited by 3 publications

(3 citation statements)

References 49 publications

(46 reference statements)

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“…Studies conducted by Fisher et al have shown that APP mRNA is present in murine oocytes and in the two-cell stage embryo, suggesting that it is one of the first genes transcribed in developing embryo [38]. Moreover, Kimura et al reported presence of APP mRNA in porcine granulosa cells from small and large follicles and soluble form of this protein in the folliular fluid [39]. The results of another study, conducted in cows, indicate that APP, among other genes, can be a helpful in oocyte developmental competence predictor [40].…”

Section: Discussionmentioning

confidence: 99%

Genes regulating programmed cell death are significantly upregulated in porcine immature oocytes

Stefańska

Józkowiak

Antosik

et al. 2019

Medical Journal of Cell Biology

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Correct maturation of the oocyte is crucial for further fertilization and embryogenesis. It comprises of both nuclear and cytoplasmic maturation, during which the proteins, nutrients and mRNAs are assembled. Cumulus cells are connected with the oocyte via gap-junctions, which enable bi-directional transfer of molecules, forming cumulus-oocyte complex (COC). The expression pattern in CCs is thought to resemble the genes expressed in the oocyte. The CCs surrounding the gamete of high developmental competence have an increased expression of apoptotic markers. Therefore, our aim in this study was to determine whether any apoptosis-related genes are upregulated in porcine oocytes before or after IVM. We isolated COCs from 45 pubertal crossbred gilts, performed brilliant cresyl blue (BCB) staining and analyzed the gene expression pattern in oocytes before and after IVM with the use of microarray analysis. The results include 419 differentially expressed transcripts, 25 of which belong to „regulation of apoptosis” and „regulation of cell death” GO BP terms. This set of genes includes BCLAF1, EIF2AK3, KLF10, MIF, MAP3K1, NOTCH2, TXNIP and APP, all of which have been upregulated in immature porcine oocytes. Our results suggest that they play part in porcine oocyte maturation and could be used as potential markers of female gamete’s developmental competence. This knowledge could serve as a basis to improve ART in pigs.

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Section: Discussionmentioning

confidence: 99%

Genes regulating programmed cell death are significantly upregulated in porcine immature oocytes

Stefańska

Józkowiak

Antosik

et al. 2019

Medical Journal of Cell Biology

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“…For instance, is APP expressed in the ovum? APP is expressed in the porcine ovary [19] and a recent report indicates that, in ovarian cancer cells, APP is the target of miR-20a [20]. Returning to the Tg2576 model, Cuadrado-Tejedor and colleagues show that in adult heterozygous mice compared with control non-transgenic littermates, the highest percentage of proteins whose levels were altered were mitochondrial proteins [21].…”

Section: App Ovum and Mitochondrial Alterationsmentioning

confidence: 99%

Maternal imprinting, mitochondrial DNA, nuclear DNA and Alzheimer’s disease

Pérez-Mediavilla

Zamarbide

2021

Explor Neuroprot Ther

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Familial early-onset Alzheimer’s disease (AD) is more probable in individuals coming from mothers diagnosed with AD than from fathers diagnosed with AD. Studies in animal models have shown maternal imprinting due to the transmission to the embryo of altered material in the ovum. In the case of transgenic animals harboring a mutated form of the human amyloid precursor protein (APP), offspring from crosses with wild-type (WT) fathers and transgenic mothers display more abnormalities than offspring from crosses with transgenic fathers and WT mothers. Expression of the mutated APP in the ovum may lead to alterations that may be genetic and/or epigenetic in the nuclear and/or the mitochondrial DNA. These modifications that are transmitted to the new living beings affect more mitochondrial proteins and, therefore, the mitochondrial function may be affected in adulthood by trends present in the ovum.

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“…The earliest study of APP in ovary could be found in Kimura et al research [11], in which he demonstrated that APP expressed in the granulosa cells and APP soluble isoforms in follicular fluid. Whereas, to date no studies have been performed about the relationship between APP and its proteolysis products and ovarian status, and the purpose of the present study is to identify APP and its proteolysis products expression in human ovaries and explore the correlation between APP and its proteolysis products and stimulation parameters as well as pregnancy outcome in women undergoing in vitro fertilization (IVF).…”

Section: Introductionmentioning

confidence: 99%