Expression of αV and β3 integrin subunits during implantation in pig

Lin, Haichao; Wang, Xin; Liu, Guifen; Fu, Jinlian; Wang, Aiguo

doi:10.1002/mrd.20732

Cited by 31 publications

(23 citation statements)

References 33 publications

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“…and Johnson et al . reported a stromal decidualization-like response in the pregnant ovine and porcine uterus by studying osteopontin, integrin αV and β3 expression [29,30]. In our study, a similar phenomenon was found.…”

Section: Resultssupporting

confidence: 85%

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Temporal and Spatial Expression of Muc1 During Implantation in Sows

Ren

Guan

et al. 2010

IJMS

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Recent evidence points to an important role for Muc1 in embryo implantation. In this study, Real-time PCR and immunohistochemistry were used to study mRNA and protein levels at, and between, the attachment sites of the endometrium of Day 13, 18 and 24 pregnant sows. The results indicate that Muc1 mRNA expression was higher between attachment sites than at attachment sites during implantation and this effect was significant on Day 13 (P < 0.01) and 24 (P < 0.01). Intense Muc1 immunostaining was observed in luminal epithelium and stroma and the staining between attachment sites was stronger than at attachment sites on Days 13 and 18. Collectively, these results suggest the crucial role of Muc1 in successful implantation and embryo survival.

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Section: Resultssupporting

confidence: 85%

“…Housekeeping gene glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was assayed as normalization control to correct for loading discrepancies for all samples assayed. Primer for GAPDH-1 was provided by BDBiosciences (Bedforld, USA); Primer for GAPDH-2 was used according to Lin et al [29]. They are listed in Table 2.…”

Section: Methodsmentioning

confidence: 99%

Temporal and Spatial Expression of Muc1 During Implantation in Sows

Ren

Guan

et al. 2010

IJMS

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“…Moreover, they found that the integrins expression may be used as a marker of endometrial receptivity and it is necessary for the proper embryo attachment during the implantation. Our observations were partially proved by Lin et al (2007). Using the pig as the model for the embryo attachment during the implantation, they showed the expression of alphaV and beta3 integrin in endometrium in all investigated stages of pig pregnancy (on Days 0, 12, 18, and 25 of pregnancy).…”

Section: Discussionsupporting

confidence: 60%

Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period

Bukowska¹,

Kempisty²,

Jackowska³

et al. 2011

Polish Journal of Veterinary Sciences

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Integrins are the major receptors within the extracellular matrix (ECM) that mediate several functions connected with cell life and metabolism, such as cell adhesion, migration, cytoskeletal organization, proliferation, survival, and differentiation. A vascular endothelial growth factor (VEGF) is one of the most important angiogenic factors. It has been suggested that the expression of this gene may play crucial physiological roles in reproductive organs.All investigated endometrial tissues were isolated on day 10-12 after mating. Control bitches, used in this study, were in metestrus, which was determined according to the vaginal cytology and progesterone level in blood. Early pregnancy was verified by flushing the uterine horns with PBS. Total RNA was isolated from the bitches endometrium by means of the Chomczyński and Sacchi method, treated by DNase I, and reverse-transcribed into cDNA. A quantitative analysis of integrins α2b, β2 and β3, VEGF 164, 182 and 188 cDNA was performed by RT-PCR.In results we have shown an increased expression of all investigated genes (integrins α2b, β2 and β3, VEGF 164, 182, and 188) in pregnant bitches uterus as compared to non-pregnant females (P<0.001).Our results indicated that the expression of genes encoding integrins and vascular endothelial growth factors is different in relation to the time of the embryo implantation and it is increased in the first period of this process. This may be associated with the induction of specific mechanisms responsible for receptivity of uterus following the embryo attachment. In addition, all of investigated genes are up-regulated in a pregnancy-specific manner and the increased expression of these genes may regulate the uterus function during the implantation of canine embryos.

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“…There is a considerable body of evidence that FAK is involved in integrin mediated signal transduction pathways (Cary and Guan, 1999; Hanks et al, 2003; Schaller, 2004; Playford et al, 2008) which correlates with the highly coordinated integrin expression in the endometrium (Aplin, 1997; Fazleabas et al, 1997; Lessey, 1997; Bowen and Hunt, 2000; Johnson et al, 2001; Lin et al, 2007; Srinivasan et al, 2009) and the blastocyst (Sutherland et al, 1993; Campbell et al, 1995; Schultz et al, 1997) in humans and other mammalian species at the time of implantation. Our laboratory has demonstrated a significant increase in integrin β3 expression along the apical plasma membrane of rat uterine luminal epithelial cells (Kaneko et al, 2011a) and in the trophoblast cells of the rat blastocyst (Kaneko et al, 2011b) at the time of implantation.…”

Section: Discussionmentioning

confidence: 99%

Focal adhesion kinase localizes to sites of cell‐to‐cell contact in vivo and increases apically in rat uterine luminal epithelium and the blastocyst at the time of implantation

Kaneko

Lecce

Day

et al. 2012

Journal of Morphology

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Focal adhesions play an important role in promoting embryo invasion; in particular, focal adhesions disassemble at the time of implantation in the rat, facilitating the detachment of the uterine luminal epithelium to allow the embryo to invade the endometrium. This study investigated focal adhesion protein, focal adhesion kinase (FAK) in the rat uterine luminal, and glandular epithelial cells to understand the dynamics of focal adhesions during early pregnancy. FAK undergoes extensive distributional change during early pregnancy, and surprisingly, FAK was not localized at the site of focal adhesions, instead being localized to the site of cell-to-cell contact and colocalizing with ZO-1 on day 1 of pregnancy. At the time of implantation, FAK increases in the apical region of the uterine luminal epithelial cells which was regulated by progesterone. Using an in vitro co-culture model of rat blastocysts attached to Ishikawa cells, FAK was present apically both in the rat blastocyst and the Ishikawa cells, suggesting a role in attachment andin mediating signal transduction between these two genetically different cell types.

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Expression of αV and β3 integrin subunits during implantation in pig

Cited by 31 publications

References 33 publications

Temporal and Spatial Expression of Muc1 During Implantation in Sows

Temporal and Spatial Expression of Muc1 During Implantation in Sows

Analysis of integrins and vascular endothelial growth factor isoforms mRNA expression in the canine uterus during perimplantation period

Focal adhesion kinase localizes to sites of cell‐to‐cell contact in vivo and increases apically in rat uterine luminal epithelium and the blastocyst at the time of implantation

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