Expression of zma-miR169 miRNAs and their target ZmNF-YA genes in response to abiotic stress in maize leaves

Luan, Mingda; Xu, Miaoyun; Lu, Yanjin; Zhang, Lan; Fan, Yanting; Wang, Lei

doi:10.1016/j.gene.2014.11.001

Cited by 105 publications

(58 citation statements)

References 32 publications

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“…), seven identified miRNAs were differentially expressed during drought, six were differentially expressed after 2 days of stress, and five were differentially expressed after 4 days of stress (Ferreira et al ). Finally, the expression patterns of zma‐miR169 and its target ZmNF‐YA in maize leaves indicated differential expression in response to short‐term (0–48 h) and long‐term (15 days) DS (Luan et al ). In agreement with the results of these previous studies, our results suggested that miRNAs from two tea cultivars and their targets responded to DS and RC in a cultivar‐ and stress level‐dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Small RNA and degradome profiling reveals important roles for microRNAs and their targets in tea plant response to drought stress

Liu

et al. 2016

Physiologia Plantarum

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Tea (Camellia sinensis) is a popular beverage worldwide. Drought stress (DS) is a major constraint on the growth, yield and quality of tea plants. MicroRNAs (miRNAs) play important roles in plant responses to DS. We constructed eight small RNA libraries from the drought-tolerant 'Ningzhou 2' (NZ2) and drought-susceptible 'Zhuyeqi' (ZYQ) cultivars during four stages [control (CK), the fourth day of DS, the eighth day of DS and after recovery (RC)]. A total of 268 conserved and 62 novel miRNAs were identified using small RNA sequencing. In total, 139 (52.9%) and 96 (36.0%) conserved miRNAs were differentially expressed during the four stages (P ≤ 0.05) in NZ2 and ZYQ, respectively. A total of 814 predicted target genes were identified as differentially regulated by 199 miRNAs through degradome sequencing. Among them, 201 and 218 genes were specific to the NZ2 and ZYQ cultivars, respectively, and 395 were common to both cultivars. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses revealed the biological roles of these targets and showed that some of the targets responded to DS in a stress- and cultivar-dependent manner. Correlated expression patterns between miRNA and their targets showed that specific miRNAs target the miRNA effector Argonaute 1 (AGO1), drought signaling-related receptors and enzymes, transcription factors, and other structural and functional proteins. The predicted regulatory networks provide insights into a potential miRNA-mediated regulatory mechanism. These results will contribute to the breeding of drought-tolerant tea plants and to elucidating miRNA regulation in response to drought.

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Section: Discussionmentioning

confidence: 99%

Small RNA and degradome profiling reveals important roles for microRNAs and their targets in tea plant response to drought stress

Liu

et al. 2016

Physiologia Plantarum

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“…The predicted targets are different types of transcription factors and functional proteins inhibited either by cleavage or translational repression. The target genes of the miR169 and miR444 families, the CAAT-box transcription factor, and the MIKC-type MADS-box transcription factor, respectively, have also been reported as target genes of maize and rice miRNAs (Yan et al 2014;Luan et al 2015). The remaining 15 target genes are unique for wheat only.…”

Section: Potential Targets Of Identified Mirnasmentioning

confidence: 99%

“…However, there are several reports indicating that miRNAs from the MIR169 family are also involved in response to biotic and abiotic stresses (Sunkar et al 2012;Inal et al 2014;Luan et al 2015). Interestingly, under drought stress the wheat miR169 was highly downregulated in leaves but upregulated in roots (Akdogan et al 2016).…”

Section: Highly Conserved Wheat Mirnas Are Developmentally Regulatedmentioning

confidence: 99%

Bioinformatic identification and expression analysis of new microRNAs from wheat (Triticum aestivum L.)

2017

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As an essential regulatory component in plants, microRNAs (miRNAs) have been intensively studied over the past decade. Although hundreds of miRNAs have been identified and analyzed in many important crops and model plants, very little is known about the function of common wheat (Triticum aestivum L.) miRNAs. In this study, we performed computational prediction of novel wheat miRNAs based on BLAST searches of the expressed sequence tag database. The expression profiles of all miRNAs were performed for both vegetative and reproductive tissues to identify developmentally regulated miRNAs. A total of 19 new miRNAs belonging to 12 MIR families were identified using stringent criteria for miRNA annotation. For all of the miRNAs, the secondary structures of their precursor sequences were predicted. Two pairs of distinct miRNAs were found to be located on the same precursor. The predicted miRNAs were experimentally verified by a stemloop qRT-PCR-based assay. The expression profiles were performed in both vegetative and reproductive tissues to find the potential correlations between the developmental phase and miRNA activity. Thirteen out of 19 miRNAs were upregulated at certain phases of plant development, and three of them (miR319, miR395, and miR171) showed the greatest expression in young spikes during microsporogenesis. Our results provide useful information for future studies of miRNA-mediated regulation of flower and grain development in wheat.

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“…miR169 which is characterized as targeting nuclear transcription factor Y (NF-Y) in several plants, such as maize and Arabidopsis , is upregulated both in heat- and cold-stressed wheat (Ni et al 2013; Gupta et al 2014; Kumar et al 2014b; Sorin et al 2014; Luan et al 2015). NF-Y, one of the most conserved transcription factors across monocots and dicots, consists of three different subunits named NF-YA, NF-YB, and NF-YC which bind to the CCAAT box in the promoter regions of target genes (Petroni et al 2012).…”

Section: Mirnas Associated With Abiotic Stress Responses In the Tritimentioning

confidence: 99%

Abiotic stress miRNomes in the Triticeae

Alptekin

Langridge

Budak

2016

Funct Integr Genomics

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The continued growth in world population necessitates increases in both the quantity and quality of agricultural production. Triticeae members, particularly wheat and barley, make an important contribution to world food reserves by providing rich sources of carbohydrate and protein. These crops are grown over diverse production environments that are characterized by a range of environmental or abiotic stresses. Abiotic stresses such as drought, heat, salinity, or nutrient deficiencies and toxicities cause large yield losses resulting in economic and environmental damage. The negative effects of abiotic stresses have increased at an alarming rate in recent years and are predicted to further deteriorate due to climate change, land degradation, and declining water supply. New technologies have provided an important tool with great potential for improving crop tolerance to the abiotic stresses: microRNAs (miRNAs). miRNAs are small regulators of gene expression that act on many different molecular and biochemical processes such as development, environmental adaptation, and stress tolerance. miRNAs can act at both the transcriptional and post-transcriptional levels, although post-transcriptional regulation is the most common in plants where miRNAs can inhibit the translation of their mRNA targets via complementary binding and cleavage. To date, expression of several miRNA families such as miR156, miR159, and miR398 has been detected as responsive to environmental conditions to regulate stress-associated molecular mechanisms individually and/or together with their various miRNA partners. Manipulation of these miRNAs and their targets may pave the way to improve crop performance under several abiotic stresses. Here, we summarize the current status of our knowledge on abiotic stress-associated miRNAs in members of the Triticeae tribe, specifically in wheat and barley, and the miRNA-based regulatory mechanisms triggered by stress conditions. Exploration of further miRNA families together with their functions under stress will improve our knowledge and provide opportunities to enhance plant performance to help us meet global food demand.

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Expression of zma-miR169 miRNAs and their target ZmNF-YA genes in response to abiotic stress in maize leaves

Cited by 105 publications

References 32 publications

Small RNA and degradome profiling reveals important roles for microRNAs and their targets in tea plant response to drought stress

Small RNA and degradome profiling reveals important roles for microRNAs and their targets in tea plant response to drought stress

Bioinformatic identification and expression analysis of new microRNAs from wheat (Triticum aestivum L.)

Abiotic stress miRNomes in the Triticeae

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