Expression of zebrafish <i>six1</i> during sensory organ development and myogenesis

Bessarab, Dmitri A.; Chong, Shang-Wei; Korzh, Vladimir

doi:10.1002/dvdy.20093

Cited by 78 publications

(67 citation statements)

References 36 publications

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“…1C,E) could represent six1 expression in the pituitary as described recently (Bessarab et al, 2004). However, we were unable to detect any six1 expression in the muscle and pectoral fin as described previously (Bessarab et al, 2004). This discrepancy could be attributable to differences in the probes used to detect six1 mRNA.…”

Section: Resultssupporting

confidence: 48%

“…The longest clone we obtained is 1088 bp long and contains a full-length ORF of 284 amino acids. The sequence of the ORF contained in this clone is identical to one recently published (Bessarab et al, 2004).…”

Section: Resultsmentioning

confidence: 62%

“…Although the expression of Six1 in the ventral otic epithelium appears to be highly conserved across vertebrates (Pandur and Moody, 2000; Xu et al, 2003;Bessarab et al, 2004), there are some striking differences between the phenotype of the Six1 knockouts in mouse Zheng et al, 2003;Ozaki et al, 2004) and its loss-of-function in zebrafish. Six1-null mutant mice inner ears never develop much beyond the otocyst stage Zheng et al, 2003;Ozaki et al, 2004).…”

Section: Discussionmentioning

confidence: 99%

“…We studied the function of the recently described zebrafish six1 (Bessarab et al, 2004) during inner ear development. In the inner ear, its expression is restricted to the ventral otocyst in which the first hair cells differentiate and prospective SAG neurons delaminate.…”

Section: Introductionmentioning

confidence: 99%

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The Transcription Factorsix1Inhibits Neuronal and Promotes Hair Cell Fate in the Developing Zebrafish (Danio rerio) Inner Ear

Bricaud¹,

Collazo²

2006

J. Neurosci.

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The developmental processes leading to the differentiation of mechanosensory hair cells and statoacoustic ganglion neurons from the early otic epithelium remain unclear. Possible candidates include members of the Pax-Six-Eya-Dach ( paired box-sine oculis homeobox-eyes absent-dachshund) gene regulatory network. We cloned zebrafish six1 and studied its function in inner ear development. Gain-and loss-of-function experiments show that six1 has opposing roles in hair cell and neuronal lineages. It promotes hair cell fate and, conversely, inhibits neuronal fate by differentially affecting cell proliferation and cell death in these lineages. By independently targeting hair cells with atoh1a (atonal homolog 1a) knockdown or neurons with neurog1 (neurogenin 1) knockdown, we showed that the remaining cell population, neurons or hair cells, respectively, is still affected by gain or loss of six1 function. six1 interacts with other members of the Pax-Six-Eya-Dach regulatory network, in particular dacha and dachb in the hair cell but not neuronal lineage. Unlike in mouse, six1 does not appear to be dependent on eya1, although it seems to be important for the regulation of eya1 and pax2b expression in the ventral otic epithelium. Furthermore, six1 expression appears to be regulated by pax2b and also by foxi1 ( forkhead box I1) as expected for an early inducer of the otic placode. Our results are the first to demonstrate a dual role for a member of the Pax-Six-Eya-Dach regulatory network in inner ear development.

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Section: Resultssupporting

confidence: 48%

Section: Resultsmentioning

confidence: 62%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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The Transcription Factorsix1Inhibits Neuronal and Promotes Hair Cell Fate in the Developing Zebrafish (Danio rerio) Inner Ear

Bricaud¹,

Collazo²

2006

J. Neurosci.

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“…This region is supposed to define a preplacodal domain that is later subdivided into distinct placodes (for review, see Baker and Bronner-Fraser, 2001;Schlosser, 2006). The expression of both eya1 and six1 is later confined to some placodes, including lateral line placodes (Sahly et al, 1999;Bessarab et al, 2004;Schlosser, 2006).…”

Section: Fate Map Of the Pll Placodementioning

confidence: 99%

Origin and Early Development of the Posterior Lateral Line System of Zebrafish

Sarrazin¹,

Nuñez²,

Sapède³

et al. 2010

Journal of Neuroscience

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The lateral line system of teleosts has recently become a model system to study patterning and morphogenesis. However, its embryonic origins are still not well understood. In zebrafish, the posterior lateral line (PLL) system is formed in two waves, one that generates the embryonic line of seven to eight neuromasts and 20 afferent neurons and a second one that generates three additional lines during larval development. The embryonic line originates from a postotic placode that produces both a migrating sensory primordium and afferent neurons. Nothing is known about the origin and innervation of the larval lines. Here we show that a "secondary" placode can be detected at 24 h postfertilization (hpf), shortly after the primary placode has given rise to the embryonic primordium and ganglion. The secondary placode generates two additional sensory primordia, primD and primII, as well as afferent neurons. The primary and secondary placodes require retinoic acid signaling at the same stage of late gastrulation, suggesting that they share a common origin. Neither primary nor secondary neurons show intrinsic specificity for neuromasts derived from their own placode, but the sequence of neuromast deposition ensures that neuromasts are primarily innervated by neurons derived from the cognate placode. The delayed formation of secondary afferent neurons accounts for the capability of the fish to form a new PLL ganglion after ablation of the embryonic ganglion at 24 hpf.

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Groucho corepressor proteins regulate otic vesicle outgrowth

Bajoghli

Aghaallaei

Czerny

2005

Developmental Dynamics

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The Groucho/Tle family of corepressor proteins is known to regulate multiple developmental pathways. Applying the dominant-negative effect of the short member Aes, we demonstrate here a critical role of this gene family also for ear development. Misexpression of Aes in medaka embryos resulted in reduced size or loss of otic vesicles, whereas overexpression of the full-length Groucho protein Tle4 gave the opposite phenotype. These results are in close agreement with phenotypes observed for eye formation, suggesting a similar role for Groucho/Tle proteins in the developmental pathways of both sensory organs. Furthermore, by using the heat-inducible HSE promoter, we observed reversible branching of the embryonic axis upon Aes misexpression, indicating a transient duplication of the organizer. Groucho proteins, therefore, are critical for organizer maintenance. Developmental Dynamics 233:760 -771, 2005.

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Expression of zebrafish six1 during sensory organ development and myogenesis

Cited by 78 publications

References 36 publications

The Transcription Factorsix1Inhibits Neuronal and Promotes Hair Cell Fate in the Developing Zebrafish (Danio rerio) Inner Ear

The Transcription Factorsix1Inhibits Neuronal and Promotes Hair Cell Fate in the Developing Zebrafish (Danio rerio) Inner Ear

Origin and Early Development of the Posterior Lateral Line System of Zebrafish

Groucho corepressor proteins regulate otic vesicle outgrowth

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