Expression of zebrafish cyp11a1 as a maternal transcript and in yolk syncytial layer

Cloning and characterization of a cDNA encoding cholesterol side-chain cleavage cytochrome P450 (CYP11A1): Tissue-distribution and changes in the transcript abundance in ovarian tissue of Japanese eel, Anguilla japonica, during artificially induced sexual development

“…Therefore, eel head kidney appears to express P450c17 although the disconnection between the abundance of the transcript and the protein needs to be addressed. In zebrafish, the transcripts of both P450scc [18] and P450c17 [34] were also detected in non-steroidogenic tissues by RT-PCR coupled with Southern blot, although the expression level was much less than that in steroidogenic tissues. This finding suggests that the non-endocrine tissues of Japanese eel potentially express the P450scc and P450c17 gene, however, the transcript abundance is low and below the detectable level of conventional RT-PCR as employed in this study.…”

“…The most distal polyadenylation signal was an ATTAAA which was located 15 nucleotides upstream from the poly (A) tail. Alignment of the amino acid residues of eel P450scc to those of the rainbow trout [17], zebrafish [18], rat [24] and human [10] forms is shown in Fig. 1.…”

“…However, the gene regulation of P450scc has received little attention in fish so far although the cDNAs encoding P450scc of rainbow trout [17], zebrafish [18] and stingray [19] have been cloned. Furthermore, there are few available reports on extra-gonadal expression of this gene in fish [18,19].…”

“…Furthermore, there are few available reports on extra-gonadal expression of this gene in fish [18,19].…”

Androgen metabolism via 17β-hydroxysteroid dehydrogenase type 3 in mammalian and non-mammalian vertebrates: comparison of the human and the zebrafish enzyme

“…In contrast to mammals, especially the molecular mechanisms underlying androgen function in fish are still widely unknown. The androgen receptors from several different fish species have been cloned and characterized at the molecular level (Ikeuchi et al 1999, Sperry & Thomas 1999, Takeo & Yamashita 1999, Touhata et al 1999, Kim et al 2002, Wilson et al 2004 and central enzymes of the general pathway leading to the formation of steroid hormones, for example P450 cholesterol side-chain cleavage enzyme (Lai et al 1998, Hsu et al 2002 and 17 -hydroxylase/17,20-lyase (Sakai et al 1992, Trant 1995, Kazeto et al 2000b, have been identified as well. Concerning enzymes of the 17 -HSD family though, only 17 -HSD type 1 from eel (Kazeto et al 2000a) and zebrafish (Mindnich et al 2004) have been studied in detail and in both species the enzyme does not seem to be involved in androgen metabolism.…”