Expression of yeast transcriptional activator MSN1 promotes accumulation of chromium and sulfur by enhancing sulfate transporter level in plants

Kim, Young Jin; Kim, Jong Hoon; Lee, Chang Eun; Mok, Young Geun; Choi, Jong Soon; Shin, Hyoung Sun; Hwang, Seongbin

doi:10.1016/j.febslet.2005.12.001

Cited by 57 publications

(20 citation statements)

References 28 publications

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“…Thus, it is available in different reactive forms, but promising species are monovalent (Cr 0), trivalent (Cr III), and the hexavalent (Cr VI) [3]. It comes into the plant body passively [4] and also through the sulphate anions as a carrier [5]. It is unspoken that Cr may not involve in any physiological processes in plants but it negatively regulates its functional behavior [6, 7].…”

Section: Introductionmentioning

confidence: 99%

Comparative transcriptome profiling of two Brassica napus cultivars under chromium toxicity and its alleviation by reduced glutathione

et al. 2016

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BackgroundChromium (Cr) being multifarious industrial used element, is considered a potential environmental threat. Cr found to be a prospective water and soil pollutant, and thus it is a current area of concern. Oilseed rape (Brassica napus L.) is well known as a major source of edible oil around the globe. Due to its higher growth, larger biomass and capability to uptake toxic materials B. napus is considered a potential candidate plant against unfavorable conditions. To date, no study has been done that described the Cr and GSH mechanism at RNA-Seq level.ResultsBoth digital gene expression (DGE) and transcriptome profile analysis (TPA) approaches had opened new insights to uncover the several number of genes related to Cr stress and GSH alleviating mechanism in two leading cultivars (ZS 758 and Zheda 622) of B. napus plants. Data showed that Cr inhibited KEGG pathways i.e. stilbenoid, diarlyheptanoid and gingerol biosynthesis; limonene and pentose degradation and glutathione metabolism in ZS 758; and ribosome and glucosinolate biosynthesis in Zheda-622. On the other hand, vitamin B6, tryptophan, sulfur, nitrogen and fructose and manose metabolisms were induced in ZS 758, and zeatin biosynthesis, linoleic acid metabolism, arginine and proline metabolism, and alanine, asparate and glutamate metabolism pathways in Zheda 622. Cr increased the TFs that were related to hydralase activity, antioxidant activity, catalytic activity phosphatase and pyrophosphatase activity in ZS 758, and vitamin binding and oxidoreductase activity in Zheda 622. Cr also up-regulated the promising proteins related to intracellular membrane bounded organelles, nitrile hyrdatase activity, cytoskeleton protein binding and stress response. It also uncovered, a novel Cr-responsive protein (CL2535.Contig1_All) that was statistically increased as compared to control and GSH treated plants. Exogenously applied GSH successfully not only recovered the changes in metabolic pathways but also induced cysteine and methionine metabolism in ZS 758 and ubiquinone and other terpenoid-quinone biosynthesis pathways in Zheda 622. Furthermore, GSH increased the level of TFs i.e. the gene expression of antioxidant and catalytic activities, iron ion binding and hydrolase activity as compared with Cr. Moreover, results pointed out a novel GSH responsive protein (CL827.Contig3_All) whose expression was found to be significantly increased when compared than Cr stress. Results further delineated that GSH induced TFs such as glutathione disulphide oxidoreducatse and aminoacyl-tRNA ligase activity, and beta glucosidase activity in ZS 758. Similarly in Zheda 622, GSH induced the TFs for instance DNA binding and protein dimerization activity. GSH also highlighted the proteins that were involved in transportation, photosynthesis process, RNA polymerase activity, and against the metal toxicity. These results indicated that cultivar ZS 758 had better metabolism and showed higher tolerance against Cr toxicity.ConclusionThe responses of ZS 758 and Zheda 622 differed consider...

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Section: Introductionmentioning

confidence: 99%

Comparative transcriptome profiling of two Brassica napus cultivars under chromium toxicity and its alleviation by reduced glutathione

et al. 2016

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“…Antioxidants have been shown to protect yeasts against the deleterious effects of Cr(VI), and Cr(VI) treatment influences the specific activities of antioxidant enzymes and the production of various ROS [36,37] (more on the antioxidant protection is described in Section 4.3). Exposure to Cr(VI) also increases the level of the Hsp 104 chaperone protein in C. intermedia, elevates the intracellular level of the oxidized form of GSH, GSSG [107,108], regulates the yeast S. cerevisiae transcriptional activator MSN1 [109], and induces intracellular signal transduction processes [110]. This indicates that oxidative cell damage plays a pivotal role in cytotoxicity [37,111], but has left the crucial targets of ROS awaiting identification [112].…”

Section: Chromium Toxicity 41 Mechanisms Of Chromium Toxicitymentioning

confidence: 96%

Interference of chromium with biological systems in yeasts and fungi: a review

Poljšak

Pócsi

Raspor

et al. 2010

J. Basic Microbiol.

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This paper deals with the interactions of chromium (Cr) with biological systems, focusing in particular on yeasts and fungi. These interactions are analysed with primarily regard to biochemical functions, but higher levels of organization are also considered. Thus, the morphological and cytological characteristics of selected microorganisms in response to exposure to chromium ions are evaluated. The different oxidation states of chromium and reactive oxygen species (ROS) generated in redox reactions with chromium ions are presented and characterized. The interactions of the most exposed subcellular structures, including the cell wall, plasma membrane and nuclei, have been deeply investigated in recent years, for two major reasons. The first is the toxicity of chromium ions and their strong impact on the metabolism of many species, ranging from microbes to humans. The second is the still disputed usefulness of chromium ions, and in particular trivalent chromium, in the glucose and fat metabolisms. Chromium pollution is still an important issue in many regions of the world, and various solutions have been proposed for the bioremediation of soil and water with selected microbial species. Yeasts and especially moulds have been most widely investigated from this aspect, and the biosorption and bioaccumulation of chromium for bioremediation purposes have been demonstrated. Accordingly, the mechanisms of chromium tolerance or resistance of selected microbes are of particular importance in both bioremediation and waste water treatment technologies. The mechanisms of chromium toxicity and detoxification have been studied extensively in yeasts and fungi, and some promising results have emerged in this area.

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“…Plant uptake of CrIII is a passive process with no energy being required (Shanker et al, 2005). However, CrVI is taken up by plants through an active mechanism probably by sulfate transport system (Kim et al, 2006). This is because CrVI, which structurally resembles sulfate and P, can affect plant nutrition, thereby interfering their uptake and translocation (GardeaeTorredey et al, 2005).…”

Section: Introductionmentioning

confidence: 98%

Chromate and phosphate inhibited each other's uptake and translocation in arsenic hyperaccumulator Pteris vittata L.

Oliveira

Lessl

Gress

et al. 2015

Environmental Pollution

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Expression of yeast transcriptional activator MSN1 promotes accumulation of chromium and sulfur by enhancing sulfate transporter level in plants

Cited by 57 publications

References 28 publications

Comparative transcriptome profiling of two Brassica napus cultivars under chromium toxicity and its alleviation by reduced glutathione

Comparative transcriptome profiling of two Brassica napus cultivars under chromium toxicity and its alleviation by reduced glutathione

Interference of chromium with biological systems in yeasts and fungi: a review

Chromate and phosphate inhibited each other's uptake and translocation in arsenic hyperaccumulator Pteris vittata L.

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