Expression of WNT genes in cervical cancer-derived cells: Implication of WNT7A in cell proliferation and migration

Ramos-Solano, Moisés; Meza-Canales, Iván David; Torres-Reyes, Luis A.; Alvarez-Zavala, Monserrat; Alvarado-Ruíz, Liliana; Rincón-Orozco, Bladimiro; García‐Chagollán, Mariel; Ochoa-Hernández, Alejandra Berenice; Ortiz‐Lazareno, Pablo César; Rösl, Frank; Gariglio, Patricio; Jave‐Suárez, Luis Felipe; Aguilar‐Lemarroy, Adriana

doi:10.1016/j.yexcr.2015.05.001

Cited by 37 publications

(50 citation statements)

References 44 publications

(58 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Tfdp1 encodes a TF that binds to the promoter regions of a number of genes whose products are involved in cell cycle regulation or in tumor proliferation (Vairapandi et al, 2002; Yasui et al, 2003; Liu S. et al, 2016; Lu et al, 2016). Bmpr2 encodes a member of the bone morphogenetic protein receptor family of transmembrane serine/threonine kinases that play important roles in stem cell differentiation (Zeng et al, 2012; Larabee et al, 2015; Ramos-Solano et al, 2015). The roles of these genes in HC regeneration remain unclear and need to be studied in the future.…”

Section: Discussionmentioning

confidence: 99%

“…Id1, Id2 , and Id3 , as mentioned above, have been reported to regulate HC formation during inner ear development (Ozeki et al, 2005; Jones et al, 2006; Laine et al, 2010; Zhan et al, 2017). Bmpr2 , as mentioned above, plays important roles in stem cell differentiation (Zeng et al, 2012; Larabee et al, 2015; Ramos-Solano et al, 2015). The roles of these genes and the Hippo pathway in HC regeneration remain unclear and need to be studied in the future.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Characterization of Lgr5+ Progenitor Cell Transcriptomes after Neomycin Injury in the Neonatal Mouse Cochlea

Zhang

Yu³

et al. 2017

Front. Mol. Neurosci.

View full text Add to dashboard Cite

Lgr5+ supporting cells (SCs) are enriched hair cell (HC) progenitors in the cochlea. Both in vitro and in vivo studies have shown that HC injury can spontaneously activate Lgr5+ progenitors to regenerate HCs in the neonatal mouse cochlea. Promoting HC regeneration requires the understanding of the mechanism of HC regeneration, and this requires knowledge of the key genes involved in HC injury-induced self-repair responses that promote the proliferation and differentiation of Lgr5+ progenitors. Here, as expected, we found that neomycin-treated Lgr5+ progenitors (NLPs) had significantly greater HC regeneration ability, and greater but not significant proliferation ability compared to untreated Lgr5+ progenitors (ULPs) in response to neomycin exposure. Next, we used RNA-seq analysis to determine the differences in the gene-expression profiles between the transcriptomes of NLPs and ULPs from the neonatal mouse cochlea. We first analyzed the genes that were enriched and differentially expressed in NLPs and ULPs and then analyzed the cell cycle genes, the transcription factors, and the signaling pathway genes that might regulate the proliferation and differentiation of Lgr5+ progenitors. We found 9 cell cycle genes, 88 transcription factors, 8 microRNAs, and 16 cell-signaling pathway genes that were significantly upregulated or downregulated after neomycin injury in NLPs. Lastly, we constructed a protein-protein interaction network to show the interaction and connections of genes that are differentially expressed in NLPs and ULPs. This study has identified the genes that might regulate the proliferation and HC regeneration of Lgr5+ progenitors after neomycin injury, and investigations into the roles and mechanisms of these genes in the cochlea should be performed in the future to identify potential therapeutic targets for HC regeneration.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of Lgr5+ Progenitor Cell Transcriptomes after Neomycin Injury in the Neonatal Mouse Cochlea

Zhang

Yu³

et al. 2017

Front. Mol. Neurosci.

View full text Add to dashboard Cite

show abstract

“…Together, these observations indicate that Wnt7a functions as a suppressor in lung cancers. Wnt7a has also been shown hypermethylated in cervical cancer, oral squamous cell cancer, pancreatic cancer, and renal cancer (Figure ).…”

Section: Roles Of Wnt7a In Human Diseasesmentioning

confidence: 96%

Roles of Wnt7a in embryo development, tissue homeostasis, and human diseases

Lan

Wang

Huang

et al. 2019

J of Cellular Biochemistry

View full text Add to dashboard Cite

Human Wnt family comprises 19 proteins which are critical to embryo development and tissue homeostasis. Binding to different frizzled (FZD) receptor, Wnt7a initiates both β‐catenin dependent pathway, and β‐catenin independent pathways such as PI3K/Akt, RAC/JNK, and extracellular signal‐regulated kinase 5/peroxisome proliferator‐activated receptor‐γ. In the embryo, Wnt7a plays a crucial role in cerebral cortex development, synapse formation, and central nervous system vasculature formation and maintenance. Wnt7a is also involved in the development of limb and female reproductive system. Wnt7a mutation leads to human limb malformations and animal female reproductive system defects. Wnt7a is implicated in homeostasis maintenance of skeletal muscle, cartilage, cornea and hair follicle, and Wnt7a treatment may be potentially applied in skeletal muscle dystrophy, corneal damage, wound repair, and hair follicle regeneration. Wnt7a plays dual roles in human tumors. Wnt7a is downregulated in lung cancers, functioning as a tumor suppressor, however, it is upregulated in several other malignancies such as ovarian cancer, breast cancer, and glioma, acting as a tumor promoter. Moreover, Wnt7a overexpression is associated with inflammation and fibrosis, but its roles need to be further investigated.

show abstract

“…In the recently published article entitled "Expression of WNT genes in cervical cancer-derived cells: Implication of Wnt7a in cell proliferation and migration", Ramos-Solano et al report the first evidence of the non-canonical Wnt7a ligand implication in CC [12].…”

mentioning

confidence: 99%

“…Ramos-Solano et al [12] analyzed the differential expression profiles between two CC-derived cell lines -HeLa and SiHa-and a human non-tumorigenic immortalized keratinocyte cell line -HaCaT-finding that 38 Wnt signaling pathway genes were up-or down modulated. Specifically, Wnt7a expression was strongly down regulated in these cell lines, as shown by qPCR and Western blot assays.…”

mentioning

confidence: 99%