Expression of VRK1 and the downstream gene BANF1 in esophageal cancer

Jin, Li; Wang, Tingting; Lü, Peng; Jing, Junpeng; Hu, Wanglai; Sun, Tiange; Liu, Hongchun

doi:10.1016/j.biopha.2017.02.095

Cited by 29 publications

(48 citation statements)

References 36 publications

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“…9 It has been reported that BANF1 may be a target for the treatment of tumors with a specialized inhibitor aimed at BAF phosphorylation mediated by vaccinia-related kinase 1. 10 Shen has reported that BANF1 was an early-stage cancer decision markers and drivers of hepatocellular carcinoma. 11 In addition, Li reported that BANF1 expression is increased in gastric cancer and associated with the poor prognosis of patients, which was a novel biomarker for gastric cancer.…”

Section: Discussionmentioning

confidence: 99%

<p>Expression and Prognostic Significance of BANF1 in Triple-Negative Breast Cancer</p>

Zhang¹

2020

CMAR

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Aim: To investigate the expression of barrier-to-autointegration factor 1 (BANF1) and its prognostic significance in triple-negative breast cancer (TNBC). Methods: BANF1 immunohistochemical detection was performed in 60 TNBC specimens and 30 normal control tissues. Real-time PCR was performed to assess the expression of BANF1 gene in TNBC tissues and their correlations with proliferation and metastasis. Kaplan-Meier survival analysis was used to assess the effect of BANF1 expression on the relapse-free survival (RFS) of TNBC patients. Univariable and multivariable Cox proportional hazards regression model analysis was used to confirm independent prognostic factors. Results: Expression of BANF1 in TNBC was significantly higher than that of the normal control group (p<0.001), and it was related to the status of lymph node metastasis and TNM staging (p<0.05), and not related to age and tumor size (p>0.05). BANF1 expression has a positive correlation with MKI67 and MTA1 expression (p<0.01). Univariable analysis showed that expression of BANF1, the status of lymph node metastasis and TNM stage were related to the relapse-free survival (RSF) of TNBC patients (p<0.001, p=0.001, p=0.013, respectively). Multivariable Cox regression indicated that the status of lymph node metastasis was an independent prognostic factor for TNBC patients (p<0.001). The survival curve suggested that the survival times for TNBC patients with high BANF1 expression have no difference compared with that for the low-expression patients (p>0.05). Conclusion: Expression of BANF1 may play a role in the occurrence and development of TNBC. Lymph node metastasis was the only independent prognostic factor predicts a poor prognosis.

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Section: Discussionmentioning

confidence: 99%

<p>Expression and Prognostic Significance of BANF1 in Triple-Negative Breast Cancer</p>

Zhang¹

2020

CMAR

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“…Some of the cytoplasmic p16 interactors identified by LC-MS/MS were selected for further examination by double immunofluorescence because of their involvement in other carcinogenic processes [23][24][25][26][27][28][29]. As seen in Figure 4C, we found that CDK4, the A-kinase anchor protein 8 (AKAP8), the barrier to autointegration factor (BANF1), and the type-1 angiotensin II receptor-associated protein (AGTRAP) colocalized with p16 in the cytoplasm of SiHa cells, confirming that these proteins interact with cytoplasmic p16 in SiHa cells.…”

Section: Colocalization Of Cytoplasmic P16 and Interactors In Siha Cellsmentioning

confidence: 99%

Absence of Nuclear p16 Is a Diagnostic and Independent Prognostic Biomarker in Squamous Cell Carcinoma of the Cervix

Mendaza

Fernández‐Irigoyen

Santamaría

et al. 2020

IJMS

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The tumor-suppressor protein p16 is paradoxically overexpressed in cervical cancer (CC). Despite its potential as a biomarker, its clinical value and the reasons for its failure in tumor suppression remain unclear. Our purpose was to determine p16 clinical and biological significance in CC. p16 expression pattern was examined by immunohistochemistry in 78 CC cases (high-grade squamous intraepithelial lesions (HSILs) and squamous cell carcinomas of the cervix –SCCCs). CC cell proliferation and invasion were monitored by real-time cell analysis and Transwell® invasion assay, respectively. Cytoplasmic p16 interactors were identified from immunoprecipitated extracts by liquid chromatography-tandem mass spectrometry, and colocalization was confirmed by double-immunofluorescence. We observed that SCCCs showed significantly more cytoplasmic than nuclear p16 expression than HSILs. Importantly, nuclear p16 absence significantly predicted poor outcome in SCCC patients irrespective of other clinical parameters. Moreover, we demonstrated that cytoplasmic p16 interacted with CDK4 and other unreported proteins, such as BANF1, AKAP8 and AGTRAP, which could sequester p16 to avoid nuclear translocation, and then, impair its anti-tumor function. Our results suggest that the absence of nuclear p16 could be a diagnostic biomarker between HSIL and SCCC, and an independent prognostic biomarker in SCCC; and explain why p16 overexpression fails to stop CC growth.

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“…According to the WHO histological classification, EC mainly comprises squamous cell carcinoma (SCC) and esophageal adenocarcinoma, of which the proportion of SCCs is as high as 90% . In China, it is estimated that the average annual death total due to EC is ~150 thousand people, which accounts for 21.8% of all cancer mortalities . Among the leading causes of death, EC ranks fourth, with an average annual incidence of 17/1 000 000 .…”

Section: Introductionmentioning

confidence: 99%

“…3,4 In China, it is estimated that the average annual death total due to EC is~150 thousand people, which accounts for 21.8% of all cancer mortalities. 5 Among the leading causes of death, EC ranks fourth, with an average annual incidence of 17/1 000 000. 6 The exact cause of EC is not clear, but it is closely related to carcinogenicity, chemical stimulation, inflammation, and trauma, as well as genetics and life habits.…”

Section: Introductionmentioning

confidence: 99%

PSMD4 regulates the malignancy of esophageal cancer cells by suppressing endoplasmic reticulum stress

Zhao

et al. 2019

The Kaohsiung J of Med Scie

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Proteasome 26S subunit non‐ATPase 4 (PSMD4) is an important proteasome ubiquitin receptor and plays a key role in endoplasmic reticulum stress (ERS). However, the study of PSMD4 in esophageal cancer (EC) is relatively rare. Here, we found that the expression of PSMD4 was markedly enhanced in EC tissues and cell lines. The cell counting kit‐8 (CCK‐8) assay showed that overexpression of PSMD4 significantly enhanced Eca109 cell viability, while inhibition of PSMD4 reduced Eca109 cell viability. Knockdown of PSMD4 induced Eca109 cell apoptosis and cell cycle arrest. More importantly, knockdown of PSMD4 significantly enhanced the expression of glucose regulated protein 78, activating transcription factor 6, and p‐protein kinase R‐like ER kinase, indicating an enhanced ERS response in esophageal cancer cells. Compared with the control cells, brefeldin A significantly inhibited the expression of PSMD4 and increased the expression of p53‐upregulated modulator of apoptosis. However, such effects were largely reversed after overexpressing PSMD4 in Eca109 cells, suggesting that silencing PSMD4 could enhance ERS‐induced cell apoptosis. In summary, upregulation of PSMD4 promoted the progression of esophageal cancer mainly by reducing ERS‐induced cell apoptosis.

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Expression of VRK1 and the downstream gene BANF1 in esophageal cancer

Cited by 29 publications

References 36 publications

<p>Expression and Prognostic Significance of BANF1 in Triple-Negative Breast Cancer</p>

<p>Expression and Prognostic Significance of BANF1 in Triple-Negative Breast Cancer</p>

Absence of Nuclear p16 Is a Diagnostic and Independent Prognostic Biomarker in Squamous Cell Carcinoma of the Cervix

PSMD4 regulates the malignancy of esophageal cancer cells by suppressing endoplasmic reticulum stress

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