Expression of virus-like particles in normal and transformed gerbil cells

Yelle, Jocelyn; Berthiaume, Laurent

doi:10.1007/bf01320785

Cited by 7 publications

(2 citation statements)

References 13 publications

(7 reference statements)

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“…Samples were processed for embedding as described previously [47]. Thin cell sections were stained with uranyl acetate 5% in 50% ethanol and with 30 mM lead citrate, and examined with a Philips EM-300 electron microscope for budding and extracellular viral particles.…”

Section: Transmission Electron Microscopymentioning

confidence: 99%

Analysis of long-term viral expression in CEM cells persistently infected with non syncytium-inducing HIV-1 strains

Yelle

Morisset

Thibodeau

1994

Archives of Virology

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CEM cells were infected with three HIV-1 non syncytium-inducing (NSI) strains obtained from AIDS patients or seropositive individuals. The surviving cells were followed for several months in the persistently infected cultures designated 65870/CEM, 65871/CEM and 3929/CEM, and analyzed for virus expression using light and electron microscopy, immunofluorescence, reverse transcriptase assay, polymerase chain reaction amplification (PCR), nucleic acid hybridization and flow cytometry. The virus isolates induced relatively few syncytia and other cytopathic effects in the corresponding cell lines and the number of cells positive for virus expression never rose above 44%. Distinct peaks of antigen-positive cells were obtained, coincident with high levels of reverse transcriptase activity. The cultures were strongly resistant to superinfection by laboratory strain Lai, with the exception of 65870/CEM which expressed HIV antigens in up to 15% of the cells for a few days. However, cell lysis was minimal in all cases. After long-term cultivation of the three cultures, no antigen-positive cells were detected and no trace of virus expression could be observed. The remaining cells consisted entirely of CD4-negative cells. PCR analyses indicated that cells harboring a provirus were progressively eliminated from the cultures, leaving only virus-free cells. In this system, cells carrying a latent provirus survive for a limited period of time before virus activation induces cell lysis. These results suggest that at least three types of cells exist in the CEM cell line: CD4-positive cells which are rapidly killed by the virus, a second type harboring a latent viral genome after the infection and which grow normally until activation of the resident genome by external or internal signal(s), and a third type which represents rare CD4-negative cells present in the initial CEM population and which are selected for by the NSI isolates. This is the first study documenting specific interactions between NSI strains of HIV-1 and distinct subpopulations of CEM cells grown as a single cell culture.

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Section: Transmission Electron Microscopymentioning

confidence: 99%

Analysis of long-term viral expression in CEM cells persistently infected with non syncytium-inducing HIV-1 strains

Yelle

Morisset

Thibodeau

1994

Archives of Virology

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“…As far as the origin of the immortalized dog cells is concerned, we noted that virus-like particles were not found in the numerous ultrathin cell sections examined todate by electron microscopy. This technique, which is the only one that can detect certain types of retroviruses [23,24] gave negative results with the JLR cells. The presence of endogenous retroviruses in dog embryo kidney cells has, in fact, never been reported in the literature.…”

Section: Properties Of Immortalized Cellsmentioning

confidence: 99%

Low tumorigenicity of canine cells transformed by the human cytomegalovirus

Yelle¹,

Lussier²,

Pramatarova³

et al. 1990

Biology of the Cell

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Dog embryo kidney cells transformed by the human cytomegalovirus (HCMV) were obtained after non-permissive infection or transfection with viral DNA digested by restriction endonuclease EcoR I. The transformed cells, growing rapidly and showing an unlimited division potential, could use medium with only 2% serum for growth, contained nuclear virus antigens, and formed small colonies (less than 0.2 mm) in agarose. From 40 mice inoculated with transformed canine cells, only one eventually developed a tumor. Results indicate that dog cells are immortalized but not tumorigenically transformed by the human cytomegalovirus.

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Retroviruses in Rodents

Kozak

Ruscetti

1992

The Retroviridae

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Expression of virus-like particles in normal and transformed gerbil cells

Cited by 7 publications

References 13 publications

Analysis of long-term viral expression in CEM cells persistently infected with non syncytium-inducing HIV-1 strains

Analysis of long-term viral expression in CEM cells persistently infected with non syncytium-inducing HIV-1 strains

Low tumorigenicity of canine cells transformed by the human cytomegalovirus

Retroviruses in Rodents

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