Expression of Vibrio harveyi ompK in the yeast Pichia pastoris: The first step in developing an oral vaccine against vibriosis?

He, Chao; Qiu, Yangyu; Chen, Jigang

doi:10.1016/j.aquaculture.2011.05.047

Cited by 17 publications

(5 citation statements)

References 33 publications

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“…Experiments used groups of sea bass (Lateolabrax japonicus) and oral uptake of OmpK in alginate microspheres for five days. The outcome was an RPS of 61.5% (Mao et al 2011). A recombinant protein vaccine, which was developed from the VirB11 gene, was trialed in groups of orange-spotted grouper.…”

Section: Vaccinesmentioning

confidence: 99%

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Zhang

Austin

2020

Mar Life Sci Technol

184

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Vibrio harveyi, which belongs to family Vibrionaceae of class Gammaproteobacteria, includes the species V. carchariae and V. trachuri as its junior synonyms. The organism is a well-recognized and serious bacterial pathogen of marine fish and invertebrates, including penaeid shrimp, in aquaculture. Diseased fish may exhibit a range of lesions, including eye lesions/blindness, gastro-enteritis, muscle necrosis, skin ulcers, and tail rot disease. In shrimp, V. harveyi is regarded as the etiological agent of luminous vibriosis in which affected animals glow in the dark. There is a second condition of shrimp known as Bolitas negricans where the digestive tract is filled with spheres of sloughed-off tissue. It is recognized that the pathogenicity mechanisms of V. harveyi may be different in fish and penaeid shrimp. In shrimp, the pathogenicity mechanisms involved the endotoxin lipopolysaccharide, and extracellular proteases, and interaction with bacteriophages. In fish, the pathogenicity mechanisms involved extracellular hemolysin (encoded by duplicate hemolysin genes), which was identified as a phospholipase B and could inactivate fish cells by apoptosis, via the caspase activation pathway. V. harveyi may enter the so-called viable but nonculturable (VBNC) state, and resuscitation of the VBNC cells may be an important reason for vibriosis outbreaks in aquaculture. Disease control measures center on dietary supplements (including probiotics), nonspecific immunostimulants, and vaccines and to a lesser extent antibiotics and other antimicrobial compounds.

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Section: Vaccinesmentioning

confidence: 99%

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Zhang

Austin

2020

Mar Life Sci Technol

184

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“…For instance, samples from rainbow trout serum, intestines, and skin mucus showed a significant increase in specific antibody titer after vaccination with encapsulated FKC and LPS of Y. ruckeri [25]. As well as significant increase in serum antibodies has been confirmed in fish that have been orally vaccinated with encapsulated antigens compared to non-coated antigens, such as rainbow trout [42,2], channel catfish [79] and sea bass [55]. However, in agreement with the previous results, the serum antibody titer against S. agalactiae was elevated in vaccinated groups through the experimental period, with the highest serum antibody titer values recorded for group A, Figure 4 shows the elevations of antibody production in the treated groups.…”

Section: Antibody Titermentioning

confidence: 90%

The Efficacy of Nile Tilapia Vaccination by Formalin killed Streptococcus agalactiae Encapsulated in Alginate Microcapsules

M Abu Darwish,

Rozaini,

Sheikh

et al. 2024

Mal. J. Fund. Appl. Sci.

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Nile tilapia is one of the major farmed fish that is often infected with Streptococcus. Oral vaccination is the most preferable technique, but the antigen is usually degraded by gastric juice. Encapsulation by alginate could be effective in protecting the antigen. The aim of this study was to assess the effectiveness of vaccinating Nile tilapia using formalin-killed Streptococcus agalactiae encapsulated in alginate. The average diameter of fabricated microcapsules was 500±20 μm, with 86% encapsulation efficacy. Fish were then divided into four groups (30 fish per group): group A: fish vaccinated by alginate microcapsules covered by formalin-killed cells (FKC), group B: fish vaccinated by non-covered FKC, group C: fish fed with empty alginate microcapsules and group D: fish feed with commercial pellets as control group. All groups were treated for 14 days and then fed with commercial pellets. Blood samples were collected on days 7, 14, 21, and 28 days post-vaccination. Bactericidal activity, lysozyme activity and serum antibody titer were significantly elevated in group A (p<0.001) compared to other treated groups. Then, gene expression analysis was performed at 29 days post-vaccination to evaluate the expression of main immune contributors such IgM, IgT, TCR β, CD4, CD8α, IL 1β, IL 8, IFN 1, and TNFα. The analysis showed significant upregulation of all tested genes in group A compared to other treated groups (p<0.001). Lastly, a challenge test was done on day 30 post-vaccination by injecting 4.6×106 CFU mL−1 of virulent S. agalactiae. The relative percent of survival (RPS) were 92± 2%, 48± 5% and 4± 3% for groups A, B and C respectively. The obtained result indicated that alginate encapsulation provided antigen protection due to its higher immunogenicity compared to non-covered vaccine. Hence, the fabricated vaccine could be incorporated with food and orally administered to Nile tilapia to prevent S. agalactiae infection.

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“…On the other hand, the administration of probiotics through the diet poses challenges, as the antigens may be degraded by proteolytic enzymes in the digestive system. To overcome this issue, increasing the probiotic dosage or encapsulating the probiotic can be considered [90,91]. Overall, the results indicate that the selected administration routes, particularly intraperitoneal and oral administration, show promise in providing protection against pathogens, and further optimization of dosages and delivery methods may enhance the efficacy of probiotics as protective agents in fish.…”

Section: Discussionmentioning

confidence: 99%

Effect of the Potential Probiotic Vibrio proteolyticus DCF12.2 on the Immune System of Solea senegalensis and Protection against Photobacterium damselae subsp. piscicida and Vibrio harveyi

Medina

García‐Márquez

Moriñigo

et al. 2023

Fishes

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Some of the characteristics of probiotics used in aquaculture are the antagonistic activity against pathogens and the activation of the immune response. Moreover, some probiotics with common antigens to pathogens can produce antibodies that react with these pathogens. One of those potential probiotics is Vibrio proteolyticus DCF12.2, a strain with the capacity to produce antibodies in soles (Solea senegalensis) that react with Photobacterium damselae subsp. piscicida and Vibrio harveyi antigens. Therefore, the objective of the work was to determine the capacity of DCF12.2 to activate the immune response in the sole, providing protection against both pathogens. For this purpose, DCF12.2 was administered to cultured soles by intraperitoneal injection, bath, and diet. The DCF12.2 strain activated the gene expression related to the fish immune response and increased the antibody production with cross-reaction to P. damselae subsp. piscicida and V. harveyi cells. Challenges with P. damselae subsp. piscicida resulted in a 30–40% relative percent survival (RPS) through DCF12.2 treatments. After challenge with V. harveyi, only intraperitoneal treatment protected the fish (67% RPS). In conclusion, the DCF12.2 strain showed great potential to be used to prevent diseases caused by both pathogens, and indicates a way to use probiotics as live vaccines.

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Expression of Vibrio harveyi ompK in the yeast Pichia pastoris: The first step in developing an oral vaccine against vibriosis?

Cited by 17 publications

References 33 publications

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

Vibrio harveyi: a serious pathogen of fish and invertebrates in mariculture

The Efficacy of Nile Tilapia Vaccination by Formalin killed Streptococcus agalactiae Encapsulated in Alginate Microcapsules

Effect of the Potential Probiotic Vibrio proteolyticus DCF12.2 on the Immune System of Solea senegalensis and Protection against Photobacterium damselae subsp. piscicida and Vibrio harveyi

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