Expression of Versican and ADAMTS During Rat Tooth Eruption

Sone, Shinya; Nakamura, Megumi; Maruya, Yuriko; Takahashi, Ichiro; Mizoguchi, Itaru; Mayanagi, Hideaki; Sasano, Yasuyuki

doi:10.1007/s10735-005-5534-2

Cited by 32 publications

(33 citation statements)

References 28 publications

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“…Osteoblasts and osteocytes may play a role in remodeling of the ECM during bone healing by coordinating the production and degradation of ECM proteins. Similar observations have been reported during bone development Tsubota et al, 2002;Maruya et al, 2003;Nakamura et al, 2005;Sone et al, 2005) and during appositional bone formation (Hatori et al, 2004).…”

Section: Discussionsupporting

confidence: 87%

Quantitative Analysis and Localization of mRNA Transcripts of Type I Collagen, Osteocalcin, MMP 2, MMP 8, and MMP 13 During Bone Healing in a Rat Calvarial Experimental Defect Model

Itagaki

Honma

Takahashi

et al. 2008

The Anatomical Record

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The study examined the expression of matrix metalloproteinases (MMPs), type I collagen and osteocalcin during bone healing in a rat calvarial experimental defect model. Twelve-week-old male Wistar rats were used. A full-thickness standardized trephine defect was made in the parietal bone, with the rat under anesthesia. RNA was extracted from tissue that filled the original bone defect on days 1 and 3 and in weeks 1, 2, 3, 5, 8, 10, 12, 18, and 24 and processed for quantitative analysis of expression of type I collagen, osteocalcin and matrix metalloproteinases (MMPs) 2, 8, and 13 by using real-time polymerase chain reaction. Alternatively, the rats were fixed by perfusion through the aorta and resected calvaria were processed for in situ hybridization for these molecules. The expression of type I collagen, osteocalcin and MMPs 2 and 13 increased toward week 2 and decreased thereafter, whereas the expression of MMP 8 was the highest on day 1. The mRNA transcripts of type I collagen and osteocalcin were localized in osteoblasts and osteocytes, some of which expressed MMPs 2, 8, and 13. Osteoblasts and osteocytes may play a role in the remodeling of extracellular matrices with MMPs during healing of a defect in bone.

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Section: Discussionsupporting

confidence: 87%

Quantitative Analysis and Localization of mRNA Transcripts of Type I Collagen, Osteocalcin, MMP 2, MMP 8, and MMP 13 During Bone Healing in a Rat Calvarial Experimental Defect Model

Itagaki

Honma

Takahashi

et al. 2008

The Anatomical Record

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“…The degradation products of the versican V1 variant were detected by Western blotting analysis in the porcine predentin matrix, and this was confirmed by ADAMTS4 digestion 23) . These results support the immunochemical detection of versican in the predentin as reported in the previous study 2) .…”

Section: Discussionmentioning

confidence: 67%

“…Several proteolytic activities are present in the predentin and dentin 1,19) , including kallikrein 4 (Klk4), matrix metalloproteinase 2(Mmp-2), Mmp-9, a disintegrin and metalloproteinase with thrombospondin motifs 4 (Adamts4), and Mmp-20 [20][21][22][23][24] . These activated proteinases degrade the coexisting proteoglycans following the addition of calcium in vitro 2) .…”

Section: Discussionmentioning

confidence: 99%

A Large Chondroitin Sulfate Proteoglycan, Versican, in Porcine Predentin

Okahata

Yamamoto

Yamakoshi

et al. 2011

Journal of Oral Biosciences

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Proteoglycans and their constituent glycosaminoglycan (GAG) have been proposed to be involved in the inhibition of mineralization in unmineralized tissue, predentin. Among the proteoglycans secreted by odontoblasts, we focused on the large chondroitin sulfate proteoglycan, versican, for its large binding capacity for calcium ions. The aims of this study were the determination of the full-length sequence and splicing variants of the porcine versican, and the detection of versican in the porcine predentin. The complete coding sequence of the porcine versican mRNA was cloned to be 11,775 nucleotides long and encode 3,924 amino acids, and four splicing variants, V0, V1, V2 and V3, were characterized in the isolated porcine cartilage cells. The number of potential GAG attachment sites was 15 in the V0 variant, 13 in the V1 variant, 2 in the V2 variant and 0 in the V3 variant. They were deposited in DDBJ. The V1 variant was determined by RT-PCR in the odontoblasts, dental papilla cells, dental follicle cells, periodontal ligament cells, dental pulp cells, and gingival cells of pigs, although a small amount of the V0 valiant was found in the dental papilla cells. The predentin was prepared from developing porcine permanent incisor tooth germs and its soluble proteins were extracted in order to be partially characterized by protein and proteinase profiles. The versican V1 cleavage products were detected in the predentin extract by Western blotting analysis. These results suggested that the versican splice variant V1 implicates both the control of the mineralization and the activities of the predentin metalloproteinases, because it has 13 GAG chains that bind a large amount of calcium.

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“…Dental pulp cells, odontoblasts, cementoblasts, cementocytes, osteoblasts, osteocytes, and periodontal ligament cells use ADAMTS5, which is one of the lytic enzymes that regulate the extracellular accumulation of proteoglycans, and ADAMTS5 plays a key role in remodeling the extracellular environment surrounding these cells (11). It has also been postulated that remodeling of proteoglycans may contribute to tooth eruption (12,13).…”

Section: Discussionmentioning

confidence: 99%

Gene expression changes in bioceramic paste-treated human dental pulp cells

Torun

Demirkaya

et al. 2016

J Oral Sci

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Abstract:We evaluated the gene expression profiles of human dental pulp cells exposed to iRoot BP using microarray after 24 and 72 h. The results were verified using quantitative reverse transcriptase PCR analysis. Of the 36,000 transcripts arrayed, 21 were up-regulated and 15 were down-regulated by more than two fold. The largest group of up-regulated genes included those involved in nucleobase-containing compound metabolic processes, cell communication, protein metabolic processes, developmental processes, and biological regulation. The largest groups of down-regulated genes were those involved in cell communication, development, and biological regulation processes. In conclusion, iRoot BP affects the expression of genes involved in different biological processes in human dental pulp cells. (J Oral Sci 58, 307-315, 2016)

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Expression of Versican and ADAMTS During Rat Tooth Eruption

Cited by 32 publications

References 28 publications

Quantitative Analysis and Localization of mRNA Transcripts of Type I Collagen, Osteocalcin, MMP 2, MMP 8, and MMP 13 During Bone Healing in a Rat Calvarial Experimental Defect Model

Quantitative Analysis and Localization of mRNA Transcripts of Type I Collagen, Osteocalcin, MMP 2, MMP 8, and MMP 13 During Bone Healing in a Rat Calvarial Experimental Defect Model

A Large Chondroitin Sulfate Proteoglycan, Versican, in Porcine Predentin

Gene expression changes in bioceramic paste-treated human dental pulp cells

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