Expression of VEGF, HGF, IL-6, IL-8, MMP-9, Telomerase in Peripheral Blood of Patients with Head and Neck Squamous Cell Carcinoma

Abstract

ObjectivesThis study investigated the telomerase expression in peripheral blood mononuclear cells (PBMCs) and the relationship between the serum level of several soluble factors such as vascular endothelial growth factor (VEGF), hepatocyte growth factor, interleukin (IL)-6, IL-8, and matrix metallopeptidase-9 and the clinicopathological features of patients with head and neck squamous cell carcinoma (HNSCC).MethodsPeripheral blood samples were collected from 50 HNSCC patients and 15 normal controls. The telomerase activity in the PBMCs was measured by Telomere Repeat Amplification Protocols. The serum levels of the soluble factors were analyzed by enzyme-linked immunosorbent assay.ResultsThe expression of telomerase in the PBMCs of HNSCC patients was significantly correlated with the N and American Joint Committee on Cancer (AJCC) stages. The serum VEGF level was significantly higher in the patients with an advanced T stage, N stage and AJCC stage. Serum VEGF was significantly related with the expression of telomerase in the PBMCs. The telomerase expression and the VEGF expression were shown to be independent factors associated with poor survival.ConclusionThe telomerase expression in the PBMCs and the serum VEGF level of HNSCC patients were significantly correlated with the N stage, the AJCC stage and the prognosis.

Serum Vascular Endothelial Growth Factor-A (VEGF-A) as a Biomarker in Squamous Cell Carcinoma of Head and Neck Patients Undergoing Chemoradiotherapy

“…In our study, serum VEGF-A level was found to be significantly higher (p=0.001) in SCCHN patients as compared to healthy controls. A similar study previously done by Riedel et al (2000), De Schutter et al (2005) and Hong et al (2009) found that serum VEGF-A level is elevated in head and neck cancer patients as compared to healthy controls.…”

“…VEGF is a multifunctional cytokine that exerts a variety of effects on vascular endothelial cells that together promote the formation of new blood vessels, increases vascular endothelial permeability, stimulates the proliferation of endothelial cells and promote cancer cell progression (Dvorak, 2002;Mohammed et al, 2007;Niu et al, 2010;Akdeniz et al, 2013;Huang et al, 2013;Zhang et al, 2013). In particular, serum VEGF-A levels are elevated in patients with HNSCC compared to healthy controls (De Schutter et al, 2005;Hong et al, 2009;Liang et al, 2013). A negative prognostic role for circulating serum VEGF-A levels has been implicated in laryngeal carcinoma (Teknos et al, 2002).…”

New Aspects of Therapy of Hepatocellular Carcinoma Egyptian Patients

“…In the current study the presence of telomerase activity in the PBMCs of HCC patients had been confirmed but after treatment with anticancer drug in added to natural oil specically Nigella Sativa oil as well as Curcumin oil the telomerase expressionnearly had been absent, and survival could be better than survival in its absence. These data had been similar to that was documented by Hong et al [79]. This could be related with a limited number of patients categorized in different clinicopathological parameters.…”

Role of MTA1 in head and neck cancers

“…Available data supports the fact that molecular therapies that inhibit the VEGF-C pathway may have advanced clinical potential in the treatment of lymphatic metastasis in HNSCC [16]. Serum levels of VEGF and MMP-9 have been shown to be helpful for making an early diagnosis and for detecting recurrence of head and neck cancers [17]. Phosphorylation and activation of p38MAP kinase is found to be important in promoting EMT during development and in tumours [18].…”