Expression of vascular endothelial growth factor and hypoxia-inducible factor-1 alpha during the periovulatory period in goats

Abstract

To evaluate the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor-1 alpha (HIF-1α) during the periovulatory period, goat ovaries were collected at 0, 4, 8, 12, 24, and 48 h after treatment with 300 IU hCG. Total cellular RNA was isolated and VEGF and HIF-1α were quantified using real-time RT-PCR. The mRNA levels of VEGF in thecal tissue remained unchanged prior to ovulation. However, the mRNA levels of VEGF in thecal tissues at 48 h increased to 7.5-fold of the level at 0 h (P < 0.05). In granulosa tissue, the VEGF mRNA levels were not detected in granulosa cells at 48 h. In thecal tissue, the mRNA level of HIF-1 1α at 4 h was 1.5-fold of the level at 0 h and remained unchanged during 8-24 h; however the levels were significantly increased at 48 h. The mRNA levels of HIF-1α in granulosa at 4 h was not changed but levels were increased (P < 0.05) at 8 h and at 12 h and then decreased at 24 and 48 h, respectively. These data describe the relationships of VEGF-and HIF-1α-dependent angiogenesis during the periovulatory periods by which hypoxia during ovulation is crucial for establishing the thecal new vasculature.

Dietary organic trace mineral supplement affects steroid hormone, antioxidant enzyme concentrations, and reproductive performances in dairy cows

“…Moreover, in our study, cows fed the OTM had a greater number of small and large follicles as well as size of large follicles. Greater P4 concentrations have a positive effect on follicular turnover increasing the number of young large follicles with the potential to ovulate and subluteal concentrations extend the growth of follicles (Navanukraw et al, 2014;Thammasiri et al, 2016).…”

Genome-wide differential mRNA expression profiles in follicles of two breeds and at two stages of estrus cycle of gilts

“…It has been reported that SULT1C3 , a member of sulfotransferase family, catalyzes the sulfate conjugation of several hormones 47 , 48 , and regulates estrogen removal in estrogen metabolic pathway 19 . In addition, HIF1ß is a critical mediator of follicle development and ovulation and a regulator of gene expression in ovary 49 , 50 . Several genes, such as INSL3 , STAR , and HIF1ß , were associated with follicular development, ovulation, and steroid secretion in the ovary of Qira black sheep at estrous and anestrous 37 .…”