Expression of vascular endothelial growth factor (VEGF) in various compartments of the human hair follicle

Kozlowska, Urszula; Blume‐Peytavi, Ulrike; Kodelja, Vitam; Sommer, Christian; Goerdt, Sergij; Majewski, Sławomir; Jabłońska, Stefania; Orfanos, Constantin E.

doi:10.1007/s004030050370

Cited by 94 publications

(77 citation statements)

References 35 publications

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“…Expression of VEGFRs may therefore indicate local VEGF activity. In the present study of expression of VEGFRs under orthophysiological conditions, localization of VEGFR-1 and -2 in endothelia adjacent to epithelia corresponded with expression of VEGF-A mRNA that has been described in some of these epithelia, such as choroid plexus, kidney glomeruli Esser et al 1998), gastrointestinal epithelium (Shifren et al 1994;Terris et al 1998), and epithelium of hair follicles (Shifren et al 1994;Kozlowska et al 1998;Yano et al 2001). Localization of VEGFR-3 in endothelium of gastrointestinal mucosa is in line with expression of VEGF-C mRNA that was found in small intestine (Joukov et al 1996).…”

Section: Discussionsupporting

confidence: 77%

Expression of Vascular Endothelial Growth Factor Receptors 1, 2, and 3 in Quiescent Endothelia

Witmer

Dai

Weich³

et al. 2002

J Histochem Cytochem.

140

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The vascular endothelial growth factor (VEGF) family is involved in angiogenesis, and therefore VEGFs are considered as targets for anti-angiogenic therapeutic strategies against cancer. However, the physiological functions of VEGFs in quiescent tissues are unclear and may interfere with such systemic therapies. In pathological conditions, increased levels of expression of the VEGF receptors VEGFR-1, VEGFR-2, and VEGFR-3 accompany VEGF activity. In this study we investigated normal human and monkey tissues for expression patterns of these receptors. Immunohistochemical staining methods at the light and electron microscopic level were applied to normal human and monkey tissue samples, using monoclonal antibodies (MAbs) against the three VEGFRs and anti-endothelial MAbs PAL-E and anti-CD31 to identify blood and lymph vessels. In human and monkey, similar distribution patterns of the three VEGFRs were found. Co-expression of VEGFR-1, −2, and −3 was observed in microvessels adjacent to epithelia in the eye, gastrointestinal mucosa, liver, kidney, and hair follicles, which is in line with the reported preferential expression of VEGF-A in some of these epithelia. VEGFR-1, −2, and −3 expression was also observed in blood vessels and sinusoids of lymphoid tissues. Furthermore, VEGFR-1, but not VEGFR-2 and −3, was present in microvessels in brain and retina. Electron microscopy showed that VEGFR-1 expression was restricted to pericytes and VEGFR-2 to endothelial cells in normal vasculature of tonsils. These findings indicate that VEGFRs have specific distribution patterns in normal tissues, suggesting physiological functions of VEGFs that may be disturbed by systemic anti-VEGF therapy. One of these functions may be involvement of VEGF in paracrine relations between epithelia and adjacent capillaries.

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Section: Discussionsupporting

confidence: 77%

Expression of Vascular Endothelial Growth Factor Receptors 1, 2, and 3 in Quiescent Endothelia

Witmer

Dai

Weich³

et al. 2002

J Histochem Cytochem.

140

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“…VEGF mRNA expression in normal human hair follicles varies during the hair cycle. It is strongly expressed in the anagen phase and decreased in the catagen and telogen phases (28,29). Our experimental results, therefore, suggest that treatment of DP cells with adenosine is effective in maintaining high IGF-1 and VEGF expressions and the ability to induce hair growth in DP cells.…”

Section: Discussionmentioning

confidence: 53%

Adenosine stimulates growth of dermal papilla and lengthens the anagen phase by increasing the cysteine level via fibroblast growth factors 2 and 7 in an organ culture of mouse vibrissae hair follicles

Hwang

Kim²,

Roh³

et al. 2011

Int J Mol Med

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Abstract. Hair regression and balding are distressing concerns for an increasing number of people due to changes in lifestyle and serious nutritional imbalances. Therapies for treatment of hair loss are needed. Among potential therapeutics, adenosine has been suggested as a potent regulator of hair growth. In this study, we investigated the effects of adenosine on hair follicles and dermal papilla (DP) cells, and the mechanism underlying the action of adenosine. Hair follicles are organs, including DP cells, that are responsible for the production of hair fibers by inducing and maintaining the hair growth phase (anagen). In a culture of DP cells in vitro, adenosine stimulated proliferation of DP cells by increasing thymidine uptake. Subsequently, adenosine activated and elongated the anagen phase by increasing the uptake of radiolabeled cysteine in an organ culture of mouse vibrissae hair follicles. We also confirmed that adenosine promoted the expression of several growth factors that are responsible for hair growth, including fibroblast growth factors (FGF)-7, FGF-2, insulin-like growth factor (IGF)-1, and vascular endothelial growth factor (VEGF) in a cDNA microarray with semi-quantitative RT-PCR. Transcriptional activation of β-catenin in DP cells was increased by adenosine in a luciferase assay. β-catenin is a co-activator of Wnt/β-catenin signaling that induces morphogenesis and differentiation of hair follicles and also acts to transactivate downstream signaling pathways, including the ERK pathway. Using Western blotting, we found that adenosine stimulated phosphorylation of ERK, CREB and AKT. These results suggest that adenosine stimulates growth of hair follicles by triggering the expression of growth factors and β-catenin, and by inducing their downstream target signaling pathways.

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“…Ангиогенный фактор VEGF в последнее время при-знают основным медиатором роста и циклического функционирования волосяного фолликула (ВФ) [8]. VEGF обнаруживается в кератиноцитах наружного эпидермального влагалища корня (НЭВК) и в фи-бробластах волосяного сосочка [9][10][11]. Ранние эта-пы анагена сопровождаются усилением экспрессии VEGF и восстановлением васкуляризации дермаль-ного сосочка фолликула [8].…”

Section: как это работает?unclassified

Minoxidil: a final judgment or a hope?

Gadzhigoroeva¹

2016

Klin. dermatol. venerol.

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ооо «Институт Красивых волос», Москва, Россия, 105082Миноксидил является базовым препаратом для наружной терапии андрогенетической алопеции у мужчин и женщин. Между тем отношение к миноксидилу неоднозначно как среди пациентов, так и среди врачей. недостаточное понимание этиологии разных форм потери волос, отсутствие ясности в механизмах интеракции миноксидила с клетками-мишенями, в особенности с клетками волосяного фолликула, а также специфичность реакции самих волос на ранних этапах лечения компрометирует действие препарата на волосы и организм человека в целом, ограничивая терапевтические возможности при лечении облысения, снижает комплаентность лечению у пациента. в статье приводится обзор научных исследований о стимулирующих механизмах миноксидила на рост волос, о клинической эффективности и преимуществе использования лекарственной формы препарата в виде пены при андрогенетической алопеции.Ключевые слова: миноксидил, пена миноксидил, андрогенетическая алопеция, лечение. Minoxidil: a final judgment or a hope?A.g. gADzHIgoRoEvA «Institute of nice Hair», Moscow, Russia, 105082Minoxidil is a basic drug for the topical treatment of androgenetic alopecia in males and females. Meanwhile, the attitude of both patients and medical professionals to minoxidil is controversial. Insufficient understanding of the etiology of various forms of hair loss, lack of clear understanding of the mechanisms of interaction between minoxidil and target cells, particularly the hair follicle cells, as well as specific reactions of the hair itself at the early stages of treatment, compromise drug effect on the hair and the entire human body, which results in limited therapeutic potential in treatment of hair loss and reduces patients' compliance. This article provides an overview of the research of stimulatory mechanisms of minoxidil on hair growth, clinical efficacy and benefits of the use of foam-like dosage form of the drug in patients with androgenetic alopecia.

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Expression of vascular endothelial growth factor (VEGF) in various compartments of the human hair follicle

Cited by 94 publications

References 35 publications

Expression of Vascular Endothelial Growth Factor Receptors 1, 2, and 3 in Quiescent Endothelia

Expression of Vascular Endothelial Growth Factor Receptors 1, 2, and 3 in Quiescent Endothelia

Adenosine stimulates growth of dermal papilla and lengthens the anagen phase by increasing the cysteine level via fibroblast growth factors 2 and 7 in an organ culture of mouse vibrissae hair follicles

Minoxidil: a final judgment or a hope?

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