Expression of Various Sialylated Carbohydrate Antigens in Malignant and Nonmalignant Pancreatic Tissues

Satomura, Yoshitake; Sawabu, Norio; Takemori, Yasuhiro; Ohta, Hiroyuki; Watanabe, Hiroyuki; Okai, Takashi; Watanabe, Kishichiro; Matsuno, Hisao; Konishi, Fumio

doi:10.1097/00006676-199107000-00012

Cited by 45 publications

(35 citation statements)

References 4 publications

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“…In chronic pancreatitis tissues, cytoplasmic staining was observed in addition to apical staining, whereas only apical staining was seen in normal pancreatic tissues. The correlation between the disturbed antigen polarity and serum level was reported to be more prominent in pancreatic cancer tissues (Satomura et al, 1991). However, a different study demonstrated no such relationship in colorectal cancer tissues for CEA and CAl9-9 (Tabuchi et al, 1988).…”

Section: Ca242 Discussionmentioning

confidence: 99%

“…The mechanisms concerning the false serum elevation of tumour markers in chronic pancreatitis have been analysed by means of immunohistochemical study. Satomura et al (1991) reported that disturbed antigen polarity plays a significant role in the elevation of serum CA19-9 levels. In chronic pancreatitis tissues, cytoplasmic staining was observed in addition to apical staining, whereas only apical staining was seen in normal pancreatic tissues.…”

Section: Ca242 Discussionmentioning

confidence: 99%

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Comparative study of CA242 and CA19-9 in chronic pancreatitis

Furuya

Kawa²,

Hasebe³

et al. 1996

Br J Cancer

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Summary CA242 has been proved to be useful in the diagnosis of pancreatic cancer. The aim of the present study was to clarify the mechanisms contributing to the high specificity of CA242 as compared with CA19-9 resulting from scarce serum elevation of this antigen in patients with chronic pancreatitis by correlating serum levels and endoscopic retrograde choledocho-pancreatography (ERCP) findings and by immunohistochemical analysis. Serum CA19-9 levels were significantly elevated in patients with calcification and with main pancreatic duct (MPD) stenosis or obstruction. On the other hand, serum CA242 levels showed no significant elevation in patients with such factors. Even though such pathological conditions were considered to lead to the stagnation of pancreatic juice, serum CA242 levels seemed to be less affected than serum CA19-9 levels. Immunohistochemical studies of chronic pancreatitis tissues revealed that CA242 was expressed less frequently and less intensely than CA19-9, and the difference in expression was more prominent in the centroacinar cells and terminal ductules. From the results of the present study, it is conceivable that CA242 is less influenced by the stagnation of the pancreatic juice than CA19-9 because of the low levels of expression in ductal systems, which results in the release of this antigen into the circulation in lower amounts than that of CA19-9.

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Section: Ca242 Discussionmentioning

confidence: 99%

Section: Ca242 Discussionmentioning

confidence: 99%

Comparative study of CA242 and CA19-9 in chronic pancreatitis

Furuya

Kawa²,

Hasebe³

et al. 1996

Br J Cancer

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“…Non-specific cytoplasmic staining with either CEA or CA19-9 has previously been demonstrated in both the normal and inflamed pancreatic ductal epithelium [35][36][37] . Performance was poor for all the markers when used as indicators for the absence of malignancy (alone or in various parallel combinations of up to four markers) as demonstrated by low NPVs and non-significant NLRs.…”

Section: Discussionmentioning

confidence: 99%

Staining for p53 and Ki-67 increases the sensitivity of EUS-FNA to detect pancreatic malignancy

Jahng

Reicher²,

Chung³

et al. 2010

WJGE

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AIM:To investigate whether tumor marker staining can improve the sensitivity of endoscopic ultrasound-guided fine needle aspiration (EUS-FNA) to diagnose pancreatic malignancy. METHODS:Patients who underwent EUS-FNA were retrospectively identified. Each EUS-FNA specimen was evaluated by routine cytology and stained for tumor markers p53, Ki-67, carcinoembryonic antigen (CEA) and CA19-9. Sensitivity, specificity, positive and negative predictive values (PPV and NPV), and positive and negative likelihood ratios (PLR and NLR) were calculated in order to evaluate the performance of each test to detect malignancy. RESULTS:Sixty-one specimens had complete sets of stains, yielding 49 and 12 specimens from pancreatic adenocarcinomas and benign pancreatic lesions due to pancreatitis, respectively. Cytology alone had sensitivity and specificity of 41% and 100% to detect malignancy, respectively. In 46% of the specimens, routine cytology alone was deemed indeterminate. The addition of either p53 or Ki-67 increased the sensitivity to 51% and 53%, respectively, with perfect specificity, PPV and PLR (100%, 100% and infinite). Both stains in combination increased the sensitivity to 57%. While additional staining with CEA and CA19-9 further increased the sensitivity to 86%, the specificity, PPV and PLR were significantly reduced (at minimum 42%, 84% and 1, respectively). Markers in all combinations performed poorly as a negative test (NPV 26% to 47%, and NLR 0.27 and 0.70). CONCLUSION: Immunohistochemical staining for p53and Ki-67 can improve the sensitivity of EUS-FNA to diagnose pancreatic adenocarcinoma.

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“…Lectins and glycan-binding antibodies have been used extensively in immunohistochemistry, for example in studies to examine the tissue distribution in pancreatic tumors of certain blood group carbohydrates (4,5). Lectins have been valuable in immunoaffinity electrophoresis and blotting methods to identify cancer-associated glycan variants on major serum proteins such as ␣-fetoprotein (6), haptoglobin (7,8), ␣ 1 -acid glycoprotein (9), and ␣ 1 -antitrypsin (10).…”

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confidence: 99%

The Prevalence and Nature of Glycan Alterations on Specific Proteins in Pancreatic Cancer Patients Revealed Using Antibody-Lectin Sandwich Arrays

Yue

Goldstein

Hollingsworth

et al. 2009

Molecular & Cellular Proteomics

114

115

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Changes to the glycan structures of proteins secreted by cancer cells are known to be functionally important and to have potential diagnostic value. However, an exploration of the population variation and prevalence of glycan alterations on specific proteins has been lacking because of limitations in conventional glycobiology methods. Here we report the use of a previously developed antibody-lectin sandwich array method to characterize both the protein and glycan levels of specific mucins and carcinoembryonic antigen-related proteins captured from the sera of pancreatic cancer patients (n ‫؍‬ 23) and control subjects (n ‫؍‬ 23). The MUC16 protein was frequently elevated in the cancer patients (65% of the patients) but showed no glycan alterations, whereas the MUC1 and MUC5AC proteins were less frequently elevated (30 and 35%, respectively) and showed highly prevalent (up to 65%) and distinct glycan alterations. The most frequent glycan elevations involved the ThomsenFriedenreich antigen, fucose, and Lewis antigens. An unexpected increase in the exposure of ␣-linked mannose also was observed on MUC1 and MUC5ac, indicating possible N-glycan modifications. Because glycan alterations occurred independently from the protein levels, improved identification of the cancer samples was achieved using glycan measurements on specific proteins relative to using the core protein measurements. The most significant elevation was the cancer antigen 19-9 on MUC1, occurring in 19 of 23 (87%) of the cancer patients and one of 23 (4%) of the control subjects. This work gives insight into the prevalence and protein carriers of glycan alterations in pancreatic cancer and points to the potential of using glycan measurements on specific proteins for highly effective biomarkers.

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Expression of Various Sialylated Carbohydrate Antigens in Malignant and Nonmalignant Pancreatic Tissues

Cited by 45 publications

References 4 publications

Comparative study of CA242 and CA19-9 in chronic pancreatitis

Comparative study of CA242 and CA19-9 in chronic pancreatitis

Staining for p53 and Ki-67 increases the sensitivity of EUS-FNA to detect pancreatic malignancy

The Prevalence and Nature of Glycan Alterations on Specific Proteins in Pancreatic Cancer Patients Revealed Using Antibody-Lectin Sandwich Arrays

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