Expression of VanA-type vancomycin resistance in a clinical isolate of Enterococcus faecium showing insertion of IS19 in the vanS gene

Silva, Beatriz Nascimento Monteiro da; Faria, Adriana Rocha; Souza, Stephanie da Silva Rodrigues; Colodette, Sabrina S; Morais, Jaqueline Martins; Teixeira, Lúcia Martins; Merquior, Vânia Lúcia Carreira

doi:10.1016/j.ijantimicag.2020.105897

Cited by 7 publications

(4 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The most common transmissible elements in enterococci are the Tn 3 family transposons, like Tn 917 (conferring resistance to MLSB antibiotics) and Tn 1546 (conferring glycopeptide resistance) and the conjugative transposon Tn 916 , which confers resistance to minocycline and tetracycline [ 49 ]. Furthermore, vancomycin resistance among enterococci most probably spread via the dissemination of variants of the vanA-type element Tn 1546 , mostly located on mobilizable or conjugative plasmids [ 50 ]. Among E. faecalis sex-pheromone inducible plasmids, carrying resistance to tetracycline, aminoglycoside and glycopeptide have also been identified [ 5 ], whereas the broad host range plasmids of the Inc18 group were found in E. faecium isolates.…”

Section: The Public Health Impact Of Vrementioning

confidence: 99%

Enterococci, from Harmless Bacteria to a Pathogen

et al. 2020

View full text Add to dashboard Cite

Enterococci are gastrointestinal commensals whose hardiness allowed them to colonize very diverse environments, including soils, water, food, and feed. This ability to overcome adverse conditions makes enterococci problematic once they colonize hospital niches. Together with the malleability of their genomes, the capacity to acquire and disseminate determinants of antibiotic resistance has contributed to converting what was once just another opportunistic pathogen into a first-class clinical problem. This review discusses the dimension of the emergence of enterococcal resistance to key antimicrobial agents, the dissemination of this resistance, and its significance in terms of public health, with the aim of raising awareness of the need to devise and implement surveillance programs and more effective antibiotic stewardship.

show abstract

Section: The Public Health Impact Of Vrementioning

confidence: 99%

Enterococci, from Harmless Bacteria to a Pathogen

et al. 2020

View full text Add to dashboard Cite

show abstract

“…We found that IS insertion may be an adaptive strategy used by bacteria to survive under the selection pressure of DAP. However, unlike other vanA genes, vanX did not show decreased expression in SC1762-D. A possible explanation is that the function or activity of vanX was not influenced by the IS insertion ( Monteiro da Silva et al, 2020 ).…”

Section: Discussionmentioning

confidence: 91%

Acquisition of Daptomycin Resistance by Enterococcus faecium Confers Collateral Sensitivity to Glycopeptides

et al. 2022

View full text Add to dashboard Cite

Daptomycin is a last-line antibiotic used in the treatment of multidrug-resistant Enterococcus faecium infections. Alarmingly, daptomycin-resistant E. faecium isolates have emerged. In this study, we investigated the evolution and mechanisms of daptomycin resistance in clinical E. faecium isolates and the corresponding acquisition of collateral sensitivity (CS) as an evolutionary trade-off. We evolved daptomycin resistance in six daptomycin-susceptible E. faecium isolates to obtain daptomycin-resistant mutants. The six E. faecium strains successfully acquired high-level resistance to daptomycin in vitro, but this led to fitness costs in terms of growth, in vitro competition, and virulence. Mutations in liaFSR, yycFG, and cls; increased surface positive charge; thicker cell walls; and elevated expression of dltABCD and tagGH were observed in daptomycin-resistant mutants. Surprisingly, we observed the emergence of CS in SC1762 isolates after the induction of daptomycin resistance. Compared with parental strains, the SC1174-D strain (i.e., daptomycin-resistant mutant of SC1174; non-CS) showed significantly upregulated expression of the vanA gene cluster. However, in SC1762-D (i.e., daptomycin-resistant mutant of SC1762), all vanA cluster genes except the vanX gene were obviously downregulated. Further in silico analyses revealed that an IS1216E-based composite transposon was generated in SC1762-D, and it disrupted the vanH gene, likely affecting the structure and expression of the vanA gene cluster and resulting in resensitization to glycopeptides. Overall, this study reports a novel form of CS between daptomycin and glycopeptides in E. faecium. Further, it provides a valuable foundation for developing effective regimens and sequential combinations of daptomycin and glycopeptides against E. faecium.

show abstract

“…The vanA resistance locus in Enterococcus, consists of a cluster of seven genes: vanS, vanR, vanH, vanA, vanX, vanY, and vanZ [51,52] that confers resistance to vancomycin and teicoplanin. The vanR along with vanS are involved in response regulation and the expression of proteins accountable for detecting the extracellular presence of antimicrobial drugs and intracellular signalling [53][54][55]. Interestingly one isolate 1EMA ADI-2802b (Day 1) had the vanRC, a vanA gene found in the vanC cluster that could have been acquired from Enterococcus innesiii.…”

Section: Discussionmentioning

confidence: 99%

Effect of Erythromycin Residuals in Food on the Development of Resistance in Streptococcus pneumoniae: An In Vivo Study in Galleria mellonella

Baranchyk,

Gestels,

Van Den Bossche

et al. 2024

Preprint

View full text Add to dashboard Cite

BackgroundThe use of antimicrobials to treat food animals may result in antimicrobial residues in foodstuffs of animal origin. The European Medicines Association (EMA) and World Health Organization (WHO) define safe antimicrobial concentrations in food based on acceptable daily intakes (ADIs). It is unknown if ADI doses of antimicrobials in food could influence the antimicrobial susceptibility of human-associated bacteria. ObjectivesThis aim of this study was to evaluate if the consumption of ADI doses of erythromycin could select for erythromycin resistance in a Galleria mellonella model of Streptococcus pneumoniae infection. Methods A chronic model of S. pneumoniae infection in G. mellonella larvae was used for the experiment. Inoculation of larvae with S. pneumoniae was followed by injections of EMA (0.0875 μg/ml) and WHO (0.012 μg/ml) ADI doses of erythromycin proceeded by isolation of S. pneumoniae colonies on selective agar plates. Minimum inhibitory concentrations (MICs) of resistant colonies were measured, and whole genome sequencing (WGS) was performed followed by variant calling to determine the genetic modifications.   Results Exposure to single doses of both EMA and WHO ADI doses of erythromycin resulted in the emergence of erythromycin resistance in S. pneumoniae. Emergent resistance to erythromycin was associated with a mutation in rplA. Conclusion In our in vivo model, even single dose of erythromycin that are classified as acceptable by the WHO and EMA induced significant increases in erythromycin MICs in S. pneumoniae. These results suggest the need to include the induction of antimicrobial resistance (AMR) as a significant criterion for determining ADIs.

show abstract

Expression of VanA-type vancomycin resistance in a clinical isolate of Enterococcus faecium showing insertion of IS19 in the vanS gene

Cited by 7 publications

References 18 publications

Enterococci, from Harmless Bacteria to a Pathogen

Enterococci, from Harmless Bacteria to a Pathogen

Acquisition of Daptomycin Resistance by Enterococcus faecium Confers Collateral Sensitivity to Glycopeptides

<strong></strong>Effect of Erythromycin Residuals in Food on the Development of Resistance in <em>Streptococcus pneumoniae</em>: An <em>In Vivo</em> Study in <em>Galleria mellonella</em>

Contact Info

Product

Resources

About