Expression of V-PPase proton pump, singly or in combination with a NHX1 transporter, in transgenic tobacco improves copper tolerance and accumulation

Gouiaa, Sandra; Khoudi, Habib

doi:10.1007/s11356-019-06852-x

Cited by 15 publications

(4 citation statements)

References 54 publications

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“…Genetically modified rice plants were reported to accumulate more Cd in their root than shoot under the overexpression of the rice V-PPase (Cao et al, 2020). The coexpression of the wheat gene for the NHX antiporter and V-PPase proton pump diminishes the toxicity of copper in transgenic tobacco (Gouiaa and Khoudi, 2019). An artificially synthesized PC gene 'PPH6HIS' was used to make the transgenic lines of the tobacco.…”

Section: Plant-microbe Interactionmentioning

confidence: 99%

Phytoremediation technologies and their mechanism for removal of heavy metal from contaminated soil: An approach for a sustainable environment

et al. 2023

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The contamination of soils with heavy metals and its associated hazardous effects are a thrust area of today’s research. Rapid industrialization, emissions from automobiles, agricultural inputs, improper disposal of waste, etc., are the major causes of soil contamination with heavy metals. These contaminants not only contaminate soil but also groundwater, reducing agricultural land and hence food quality. These contaminants enter the food chain and have a severe effect on human health. It is important to remove these contaminants from the soil. Various economic and ecological strategies are required to restore the soils contaminated with heavy metals. Phytoremediation is an emerging technology that is non-invasive, cost-effective, and aesthetically pleasing. Many metal-binding proteins (MBPs) of the plants are significantly involved in the phytoremediation of heavy metals; the MBPs include metallothioneins; phytochelatins; metalloenzymes; metal-activated enzymes; and many metal storage proteins, carrier proteins, and channel proteins. Plants are genetically modified to enhance their phytoremediation capacity. In Arabidopsis, the expression of the mercuric ion-binding protein in Bacillus megaterium improves the metal accumulation capacity. The phytoremediation efficiency of plants is also enhanced when assisted with microorganisms, biochar, and/or chemicals. Removing heavy metals from agricultural land without challenging food security is almost impossible. As a result, crop selections with the ability to sequester heavy metals and provide food security are in high demand. This paper summarizes the role of plant proteins and plant–microbe interaction in remediating soils contaminated with heavy metals. Biotechnological approaches or genetic engineering can also be used to tackle the problem of heavy metal contamination.

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Section: Plant-microbe Interactionmentioning

confidence: 99%

Phytoremediation technologies and their mechanism for removal of heavy metal from contaminated soil: An approach for a sustainable environment

et al. 2023

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“…By controlling the synthesis of lignin, which serves as an apoplast barrier to prevent arsenate from entering root cells and reduces its accumulation in transgenic plant, OsPRX38 overexpression in transgenic rice greatly enhanced arsenic tolerance. In transgenic tobacco, the co-expression of the wheat-gene encoding the vacuolar proton pumps (V-PPase proton pump) and sodium/proton antiporter (NHX antiporter) reduces copper toxicity (Gouiaa & Khoudi, 2019) [17] . To create the transgenic tobacco lines, the phytochekatin gene "PPH6HIS" was synthetically created.…”

Section: Transgenic Plantsmentioning

confidence: 99%

Approaches for alleviating heavy metal stress

Kaur,

Yadav

2023

Pharma Innovation

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Anthropogenic activities have led an augment in heavy metal contamination in water and soil throughout the past few decades. Increased crop plant exposure to heavy metals reduces production and growth, which affects agricultural production's future viability. Therefore, there is an urgent requirement for various strategies for mitigation of heavy metal stress. Among them, silicon supplementation presents a promising role in mitigating heavy metal stress. Numerous types of microorganisms, including fungi and bacteria are used as effective natural bioactive stimulants in the rhizosphere to help plants stressed by heavy metals. Nanoparticles (NPs) are useful for reducing heavy metal stress because of their distinct physiochemical characteristics. Plants can be used as hyper-accumulators of heavy metal ions, which is beneficial both economically and practically. Plant genetic modification techniques are used to increase phytoremediation's potential. The use of these strategies may prove to be economical and sustainable method for heavy metal detoxification.

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“…Gouiaa et al (2012) demonstrated that co‐overexpression in tobacco of the V‐PPase TaVP1 and the vacuolar sodium antiporter TaNHXS1 resulted in enhanced tonoplast V‐PPase, V‐ATPase, and Na + /H + transport activities. The single TaVP1 transgenic tobacco lines exhibited better tolerance and accumulated higher quantities of Cu than their WT counterparts (Gouiaa & Khoudi 2019). Interestingly, the double transgenic tobacco plants, co‐expressing V‐PPase and sodium antiporter, outperformed the single transgenic TaVP1 plants and accumulated significantly more Cu in their areal parts.…”

Section: Overexpression Of Vacuolar Proton Pumps As a Novel Strategy To Enhance Heavy Metal Tolerance And Phytoremediationmentioning

confidence: 99%

Significance of vacuolar proton pumps and metal/H ⁺ antiporters in plant heavy metal tolerance

Khoudi

2021

Physiologia Plantarum

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Soil and water are among the most valuable resources on earth. Unfortunately, their contamination with heavy metals has become a global problem. Heavy metals are not biodegradable and cannot be chemically degraded; therefore, they tend to accumulate in soils or to be transported by streaming water and contaminate both surface and groundwater. Cadmium (Cd) has no known biological function but is one of the most toxic metals. It represents a serious environmental concern since its accumulation in soils is associated with health risks to plants, animals and humans. On the other hand, copper (Cu) and zinc (Zn) are heavy metals that are indispensable to plants but become toxic when their concentration in soils exceeds a certain optimal level. Plants have evolved many mechanisms to cope with heavy metal toxicity; vacuolar sequestration is one of them. Vacuolar sequestration can be achieved through either phytochelatin‐dependent or phytochelatin‐independent pathways. Most of the transgenic plants meant for phytoremediation described in the literature result from the manipulation of genes involved in the phytochelatin‐dependent pathway. However, recent evidence has emerged to support the importance of the phytochelatin‐independent pathway in heavy metal sequestration into the vacuole, with metal/H+ antiporters and proton pumps playing an important role. In this review, the importance of vacuolar proton pumps and metal/H+ antiporters transporting Cd, Cu, and Zn is discussed. In addition, the recent advances in the production of transgenic plants with potential application in phytoremediation and food safety through the manipulation of genes encoding V‐PPase proton pumps is described.

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Expression of V-PPase proton pump, singly or in combination with a NHX1 transporter, in transgenic tobacco improves copper tolerance and accumulation

Cited by 15 publications

References 54 publications

Phytoremediation technologies and their mechanism for removal of heavy metal from contaminated soil: An approach for a sustainable environment

Phytoremediation technologies and their mechanism for removal of heavy metal from contaminated soil: An approach for a sustainable environment

Approaches for alleviating heavy metal stress

Significance of vacuolar proton pumps and metal/H ⁺ antiporters in plant heavy metal tolerance

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Expression of V-PPase proton pump, singly or in combination with a NHX1 transporter, in transgenic tobacco improves copper tolerance and accumulation

Cited by 15 publications

References 54 publications

Phytoremediation technologies and their mechanism for removal of heavy metal from contaminated soil: An approach for a sustainable environment

Phytoremediation technologies and their mechanism for removal of heavy metal from contaminated soil: An approach for a sustainable environment

Approaches for alleviating heavy metal stress

Significance of vacuolar proton pumps and metal/H + antiporters in plant heavy metal tolerance

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Significance of vacuolar proton pumps and metal/H ⁺ antiporters in plant heavy metal tolerance