Expression of tyrosine hydroxylase in cerebellar Purkinje neurons of the mutant tottering and leaner mouse

Patterned Purkinje cell degeneration in mouse models of Niemann‐Pick type C disease

“…13 in Yadid et al, 1998). Similar ectopic TH expression has been seen in Purkinje cells of other ataxic mutant mice (e.g., tottering, leaner: Hess and Wilson, 1991;Austin et al, 1992;Fletcher et al, 1996;pogo: Jeong et al, 2001), and, as in NPC1, it is primarily detected in the zebrin II-immunoreactive Purkinje cell subtype prior to Purkinje cell degeneration. The significance of the TH expression is unclear; although it is clearly not a prelude to death (for example, TH immunoreactivity is rarely detected in zebrin II-negative Purkinje cells, which are the first to die), neither is it consistently correlated with Purkinje cell survival.…”

The Secretory Granule-Associated Protein CAPS2 Regulates Neurotrophin Release and Cell Survival

“…CAPS2 might control the release of such catecholamines. However, cerebellar granule cells express neither tyrosine hydroxylase, the first enzymatic step in catecholamine synthesis (Austin et al, 1992;Takada et al, 1993), nor dopamine-␤-hydroxylase (Verney et al, 1982), indicating that CAPS2 is involved in the release of substances, except for catecholamines, in the vesicles of PF terminals. We then showed that exogenously expressed CAPS2 promotes the NT-3 and BDNF release from PC12 cells and NT-3 release from granule cells in a depolarization-dependent manner.…”

Homer 1a Gene Expression Modulation by Antipsychotic Drugs Involvement of the Glutamate Metabotropic System and Effects of D-Cycloserine

“…The unincorporated nucleotides were separated from radiolabeled DNA using Sephadex G-50 chromatographic columns (Amersham Pharmacia Biotech). The procedure for in situ hybridization histochemistry was taken from several standard published protocols (Austin et al 1992;de Bartolomeis et al 1994;Young et al 1986). …”