Expression of two-pore domain potassium channels in nonhuman primate sperm

Chow, Gregory E.; Müller, Charles; Curnow, Eliza; Hayes, Eric

doi:10.1016/j.fertnstert.2006.06.051

Cited by 8 publications

(10 citation statements)

References 49 publications

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“…In addition, a previous study from our laboratory also found these channels to be expressed in bovine ovaries, testis, and germ cells (Hur et al 2009). Furthermore, they were also present in monkeys, where their expression was restricted to sperm (Chow et al 2007). In this study, the patterns of each K 2P channel mRNA expression showed embryo stage-specific effects.…”

Section: Discussionsupporting

confidence: 50%

“…Recent studies have found that K 2P channels are expressed in the mammalian reproductive organ and cells, such as in human cytotrophoblast cells, placental villous tissue and trophoblast cells, myometrium, the placental vascular system, and uterine smooth muscle cells (Bai et al 2005a, 2005b, Tichenor et al 2005, Wareing et al 2006, Chow et al 2007. In addition, a previous study from our laboratory also found these channels to be expressed in bovine ovaries, testis, and germ cells (Hur et al 2009).…”

Section: Discussionmentioning

confidence: 78%

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K+ efflux through two-pore domain K+ channels is required for mouse embryonic development

Hur

Kim²,

Cho³

et al. 2012

Reproduction

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Numerous studies have suggested that K C channels regulate a wide range of physiological processes in mammalian cells. However, little is known about the specific function of K C channels in germ cells. In this study, mouse zygotes were cultured in a medium containing K C channel blockers to identify the functional role of K C channels in mouse embryonic development. Voltage-dependent K C channel blockers, such as tetraethylammonium and BaCl 2 , had no effect on embryonic development to the blastocyst stage, whereas K 2P channel blockers, such as quinine, selective serotonin reuptake inhibitors (fluoxetine, paroxetine, and citalopram), gadolinium trichloride, anandamide, ruthenium red, and zinc chloride, significantly decreased blastocyst formation (P!0.05). RT-PCR data showed that members of the K 2P channel family, specifically KCNK2, KCNK10, KCNK4, KCNK3, and KCNK9, were expressed in mouse oocytes and embryos. In addition, their mRNA expression levels, except Kcnk3, were up-regulated by above ninefold in morula-stage embryos compared with 2-cell stage embryos (2-cells). Immunocytochemical data showed that KCNK2, KCNK10, KCNK4, KCNK3, and KCNK9 channel proteins were expressed in the membrane of oocytes, 2-cells, and blastocysts. Each siRNA injection targeted at Kcnk2, Kcnk10, Kcnk4, Kcnk3, and Kcnk9 significantly decreased blastocyst formation by w38% compared with scrambled siRNA injection (P!0.05). The blockade of K 2P channels acidified the intracellular pH and depolarized the membrane potential. These results suggest that K 2P channels could improve mouse embryonic development through the modulation of gating by activators.

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Section: Discussionsupporting

confidence: 50%

Section: Discussionmentioning

confidence: 78%

K+ efflux through two-pore domain K+ channels is required for mouse embryonic development

Hur

Kim²,

Cho³

et al. 2012

Reproduction

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“…This challenge stems from the fact that Trek-1 is known to be insensitive to classical K ϩ channel inhibitors including glibenclamide, 4-aminopyridine, and tetraethylammonium (35,44). Other pharmacological approaches including amiloride, gadolininum, and riluzuole have been criticized as nonspecific (5,18,24,34,43,46). Therefore, to selectively inhibit Trek-1, we created a stable Trek-1 shRNAtransfected MLE-12 cell line.…”

Section: Discussionmentioning

confidence: 99%

Regulation and function of the two-pore-domain (K2P) potassium channel Trek-1 in alveolar epithelial cells

Schwingshackl

Teng

Ghosh

et al. 2012

American Journal of Physiology-Lung Cellular and Molecular Phys

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Hyperoxia can lead to a myriad of deleterious effects in the lung including epithelial damage and diffuse inflammation. The specific mechanisms by which hyperoxia promotes these pathological changes are not completely understood. Activation of ion channels has been proposed as one of the mechanisms required for cell activation and mediator secretion. The two-pore-domain K(+) channel (K2P) Trek-1 has recently been described in lung epithelial cells, but its function remains elusive. In this study we hypothesized that hyperoxia affects expression of Trek-1 in alveolar epithelial cells and that Trek-1 is involved in regulation of cell proliferation and cytokine secretion. We found gene expression of several K2P channels in mouse alveolar epithelial cells (MLE-12), and expression of Trek-1 was significantly downregulated in cultured cells and lungs of mice exposed to hyperoxia. Similarly, proliferation cell nuclear antigen (PCNA) and Cyclin D1 expression were downregulated by exposure to hyperoxia. We developed an MLE-12 cell line deficient in Trek-1 expression using shRNA and found that Trek-1 deficiency resulted in increased cell proliferation and upregulation of PCNA but not Cyclin D1. Furthermore, IL-6 and regulated on activation normal T-expressed and presumably secreted (RANTES) secretion was decreased in Trek-1-deficient cells, whereas release of monocyte chemoattractant protein-1 was increased. Release of KC/IL-8 was not affected by Trek-1 deficiency. Overall, deficiency of Trek-1 had a more pronounced effect on mediator secretion than exposure to hyperoxia. This is the first report suggesting that the K(+) channel Trek-1 could be involved in regulation of alveolar epithelial cell proliferation and cytokine secretion, but a direct association with hyperoxia-induced changes in Trek-1 levels remains elusive.

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“…In reproductive cell types, the presence and function of K 2P channels were also reported (Bai et al 2005a, 2005b, Tichenor et al 2005, Wareing et al 2006, Chow et al 2007. However, the expression and localization of the K 2P channels in bovine germ cells have not yet been reported.…”

Section: Introductionmentioning

confidence: 93%

“…KCNK2 and KCNK4 are also expressed in human uterine smooth muscle, and KCNK2 expression is significantly increased in pregnant term tissues (Tichenor et al 2005). KCNK5, KCNK2, and KCNK4 are expressed by and localized specifically to monkey sperm (Chow et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Expression and localization of two-pore domain K+ channels in bovine germ cells

Hur¹,

Choe²,

Kim³

et al. 2009

Reproduction

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Two-pore domain K C (K 2P ) channels that help set the resting membrane potential of excitable and nonexcitable cells are expressed in many kinds of cells and tissues. However, the expression of K 2P channels has not yet been reported in bovine germ cells. In this study, we demonstrate for the first time that K 2P channels are expressed in the reproductive organs and germ cells of Korean cattle. RT-PCR data showed that members of the K 2P channel family, specifically KCNK3, KCNK9, KCNK2, KCNK10, and KCNK4, were expressed in the ovary, testis, oocytes, embryo, and sperm. Out of these channels, KCNK2 and KCNK4 mRNAs were abundantly expressed in the mature oocytes, eight-cell stage embryos, and blastocysts compared with immature oocytes. KCNK4 and KCNK3 were significantly increased in eight-cell stage embryos. Immunocytochemical data showed that KCNK2, KCNK10, KCNK4, KCNK3, and KCNK9 channel proteins were expressed at the membrane of oocytes and blastocysts. KCNK10 and KCNK4 were strongly expressed and distributed in oocyte membranes. These channel proteins were also localized to the acrosome sperm cap. In particular, KCNK3 and KCNK4 were strongly localized to the post-acrosomal region of the sperm head and the equatorial band within the sperm head respectively. These results suggest that K 2P channels might contribute to the background K C conductance of germ cells and regulate various physiological processes, such as maturation, fertilization, and development.

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Expression of two-pore domain potassium channels in nonhuman primate sperm

Abstract: The results demonstrated expression and function of K(2P) potassium channels in nonhuman primate sperm for the first time. The unique, discrete distributions of K(2P) channels in nonhuman primate sperm suggest specific roles for this subfamily of ion channels in primate sperm function.

Cited by 8 publications

References 49 publications

K+ efflux through two-pore domain K+ channels is required for mouse embryonic development

K+ efflux through two-pore domain K+ channels is required for mouse embryonic development

Regulation and function of the two-pore-domain (K2P) potassium channel Trek-1 in alveolar epithelial cells

Expression and localization of two-pore domain K+ channels in bovine germ cells

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