Expression of TTF‐1 and cytokeratins in primary and secondary epithelial lung tumours: correlation with histological type and grade

Marson, V.; Mazières, Julien; Groussard, O; Garcia, Odalys Lara; Berjaud, J.; Dahan, Marcel; Carlès, Pierre; D'aste, G

doi:10.1111/j.1365-2559.2004.01893.x

Cited by 118 publications

(30 citation statements)

References 26 publications

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“…TTF-1 is generally expressed diffusely in thyroid tumors but TTF-1 immunoreactivity varies according to tumor type in lung tumors (6). The TTF-1 immunoreactivity rate in primary lung ACs has been reported to be 60% to 100% (6,(38)(39)(40)(41). Studies reporting a lower staining percentage have emphasized the reason as TTF-1 immunoreactivity being lower in mucinous ACs compared to nonmucinous cases (38)(39)(40)(41)(42).…”

Section: Discussionmentioning

confidence: 92%

“…TTF-1 is also used as a marker in the differential diagnosis of lung adenocarcinomas and pleural mesothelioma (6,36). While TTF-1 expression is less frequently seen (0-30%) in LCCs of the lung (39,40,44), this rate is even lower (0-21%) in SCCs of the lung (6,36,(38)(39)(40)44). …”

Section: Discussionmentioning

confidence: 99%

“…The TTF-1 immunoreactivity rate in primary lung ACs has been reported to be 60% to 100% (6,(38)(39)(40)(41). Studies reporting a lower staining percentage have emphasized the reason as TTF-1 immunoreactivity being lower in mucinous ACs compared to nonmucinous cases (38)(39)(40)(41)(42). TTF-1 is widely utilized in the identification of ACs of lung origin as TTF-1 has been determined to be negative in extra-pulmonary ACs other than some rare ovarian carcinomas (6,43).…”

Section: Discussionmentioning

confidence: 99%

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Expression of p63, ttf–1 and maspin in non-small cell lung carcinoma and their effect on the prognosis and differential diagnosis

Yaman¹,

Nart²,

Ekren³

et al. 2015

TJPATH

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Objective: Lung cancer is still the leading cause of cancer mortality. Antiapoptotic genes and protease inhibitors play an important role in the development of lung cancer.Material and Method: p63, TTF-1 and maspin expression and their role in the differential diagnosis, overall survival, progression-free survival and other clinicopathological characteristics of the patients were investigated in 80 surgically-resected non-small cell lung carcinomas. Results:The maximal tumor diameter range was 1.5-11 cm (mean: 4.06±1.8 cm). Forty-five (56.3%) tumors were adenocarcinoma, 23 (28.8%) squamous cell carcinoma, four (5%) large cell carcinoma, six (7.5%) large cell neuroendocrine carcinoma, one (1.2%) sarcomatoid carcinoma while one was (1.2%) both adenocarcinoma and squamous cell carcinoma. The patients with advanced TNm stage and a tumor diameter more than 3 cm had markedly poor survival. Immunohistochemically, p63 staining was present in 87.5% of squamous cell carcinomas, 4.3% of adenocarcinomas, 25% of large cell carcinomas, and 16.7% of large cell neuroendocrine carcinomas. Similarly, maspin was positive in 66.7% of squamous cell carcinomas and 17.4% of adenocarcinomas. The TTF-1 staining rate was higher in adenocarcinomas (84.8%). There was no immunoreactivity in squamous cell carcinomas (p<0.001). We found that p63 and TTF-1 had no significant effect on survival in either tumor group (p>0.05) while maspin has a negative prognostic effect in adenocarcinoma (p=0.048). Conclusion:This study suggests that p63 and TTF-1 are reliable markers in non-small cell lung carcinoma and can be used in differential diagnosis. maspin has been identified as a prognostic marker in adenocarcinoma. However, more studies are required to elucidate the significance of maspin.Key Words: Non-small-cell lung carcinoma, p63, TTF-1, maspin, Prognosis ÖZ Amaç: Akciğer kanseri, kanser ölümlerinin önde gelen nedenlerindendir. Antiapopitotik genler ve proteaz inhibitörleri akciğer kanseri gelişiminde önemli role sahiptir. Gereç ve Yöntem:Seksen opere küçük hücreli dışı akciğer karsinom olgusunda p63, TTF-1 ve maspin ekspresyonunun ayırıcı tanıya olan katkıları, sağkalım ve hastalıksız sağkalım üzerine etkileri ve diğer klinikopatolojik özellikler değerlendirildi. Bulgular:Ameliyat materyallerinde izlenen tümörlerin çapları 1,5-11 cm arasında (ort. 4,06±1,8) olup, 45 (%56,3) adenokarsinom, 23 (%28,8) skuamöz hücreli karsinom, dört (%5) büyük hücreli karsinom, altı (%7,5) büyük hücreli nöroendokrin karsinom, bir (%1,2) sarkomatoid karsinom ve bir (%1,2) adenokarsinom ve skuamöz hücreli karsinom birlikteliğinden oluşmakta idi. İleri TNm evreli ve üç cm' den daha büyük tümör çapı olan olgular daha kötü prognozlu idi. İmmünohistokimyasal incelemede skuamöz hücreli karsinomların %87,5'i, adenokarsinomların %4,3'ü, büyük hücreli karsinomların %25'i ve büyük hücreli nöroendokrin karsinomların %16,7'si p63 ile pozitif boyandı. Benzer olarak maspin de skuamöz hücreli karsinomlarda %66,7 ve adenokarsinomlarda %27,4 oranında pozitifti. TTF-1 ekspre...

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Section: Discussionmentioning

confidence: 92%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Expression of p63, ttf–1 and maspin in non-small cell lung carcinoma and their effect on the prognosis and differential diagnosis

Yaman¹,

Nart²,

Ekren³

et al. 2015

TJPATH

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“…Although many types of nonpulmonary adenocarcinomas (such as endometrium, pancreas, breast and ovary) may be less than 50% positive for CK5/6 [43], it has been reported to be a sensitive and specific marker of lung squamous cell carcinomas (at 73-100% in tissue sections [11,44,45,46,47] and at 75-100% in cytology [41,48,49]). In our study, CK5/6 showed the best possible specificity (100%) for squamous cell carcinoma, but the sensitivity was 81%.…”

Section: Discussionmentioning

confidence: 99%

Expressions of Thyroid Transcription Factor-1, Napsin A, p40, p63, CK5/6 and Desmocollin-3 in Non-Small Cell Lung Cancer, as Revealed by Imprint Cytology Using a Malinol-Based Cell-Transfer Technique

Kawai

Tominaga²,

Hiroi³

et al. 2015

Acta Cytologica

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Background: The introduction of new therapies has made it important to differentiate between adenocarcinoma and squamous cell carcinoma. To allow the use of various immunocytochemical stains on limited materials, we tried transferring cells from a given smear to multiple slides. Using touch-preparation samples of 215 surgically resected non-small cell lung carcinomas of confirmed histologic classification (adenocarcinoma,n = 101; squamous cell carcinoma,n = 114), we performed immunocytochemistry for thyroid transcription factor-1, napsin A, p40, p63, CK5/6 and desmocollin-3, and compared cytologic staining results with the corresponding resection. Methods: We examined: (a) the expressions of the above 6 antibodies on cells transferred from touch imprints of resected specimens, the extent of staining being considered positive if more than 5% of the area was stained, and (b) the sensitivity, specificity, positive predictive value and negative predictive value for each antibody. Results: The histologic corresponding rate with Papanicolaou staining was only 73%. Regarding the differentiation of adenocarcinoma from squamous cell carcinoma, the sensitivity and specificity for napsin A in adenocarcinoma were 80 and 97%, respectively, while those for p40 in squamous cell carcinoma were 84 and 98%, respectively. Conclusion: The immunocytochemical expressions of napsin A and p40 in imprint cytology seem to be of great utility for the accurate histological differentiation of lung cancers.

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“…Further, their literature review indicated earlier studies with the same clone had not shown any positive staining among 131 primary CRCs and 52 metastatic CRC to the lung or liver 2. More recently, the 8G7G3/1 antibody has been reported not to produce any staining among 163 and 134 intrapulmonary CRC metastases and 50 primary CRCs 5. Despite all these opposing existing data, we have no doubts about the legitimacy of our findings because positive immunostaining was consistently reproduced despite our use of different antigen retrieval, primary antibody concentrations and detection systems.…”

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confidence: 95%

Positive immunostaining for thyroid transcription factor-1 in colorectal adenocarcinoma using the 8G7G3/1 monoclonal antibody

et al. 2008

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Expression of TTF‐1 and cytokeratins in primary and secondary epithelial lung tumours: correlation with histological type and grade

Abstract: Immunohistochemical expression of CKs and TTF-1 may be correlated with histological type and grade of lung primary epithelial tumours and may allow them to be distinguished from non-pulmonary carcinomas.

Cited by 118 publications

References 26 publications

Expression of p63, ttf–1 and maspin in non-small cell lung carcinoma and their effect on the prognosis and differential diagnosis

Expression of p63, ttf–1 and maspin in non-small cell lung carcinoma and their effect on the prognosis and differential diagnosis

Expressions of Thyroid Transcription Factor-1, Napsin A, p40, p63, CK5/6 and Desmocollin-3 in Non-Small Cell Lung Cancer, as Revealed by Imprint Cytology Using a Malinol-Based Cell-Transfer Technique

Positive immunostaining for thyroid transcription factor-1 in colorectal adenocarcinoma using the 8G7G3/1 monoclonal antibody

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