Expression of TSLP and IL-33 receptors on sputum macrophages of asthma patients and healthy subjects

Paplińska-Goryca, Magdalena; Nejman-Gryz, Patrycja; Proboszcz, Małgorzata; Kwiecień, Iwona; Hermanowicz-Salamon, Joanna; Grabczak, Elżbieta M.; Krenke, Rafał

doi:10.1080/02770903.2018.1543435

Cited by 14 publications

(9 citation statements)

References 40 publications

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“…36 IL-10R2/IL-20R1 the heterodimeric receptor of IL-26 is expressed on the surface of epithelial bronchial cells 13 suggesting a possible activation of these cells by IL-26 following non-specific epithelium aggression, as these cells produce important levels of IL-33 and TSLP in asthmatic patients. 47,48 The addition of IL-17 and IL-26 to alarmin and TSLP could play an active role in the pathogenesis of asthma by stimulating mononuclear cells in establishing a pro-inflammatory micro-environment.…”

Section: Discussionmentioning

confidence: 99%

Sputum IL-26 Is Overexpressed in Severe Asthma and Induces Proinflammatory Cytokine Production and Th17 Cell Generation: A Case–Control Study of Women

Louhaichi¹,

Mlika²,

Hamdi³

et al. 2020

JAA

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Objective: Asthma inflammation is a complex pathway involving numerous mediators. Interleukin-26 (IL-26), a member of the IL-10 cytokine family, is abundant in human airways and induces the production of proinflammatory cytokines. Our aim was to investigate the possible role of IL-26 in severe asthma. We analysed the expression of IL-26 in severe asthma both in peripheral blood and induced sputum. Patients and Methods: A total of 50 adult women with severe asthma were recruited and compared to 30 healthy controls (HC). Serum and sputum fluid (SF) levels of IL-26 and IL-17 were defined by ELISA. IL-26 mRNA expression and IL-26 protein were analysed using RT-PCR and Western blot. In vitro, we studied the effect of recombinant IL-26 (rIL-26) and SF-IL-26 on cultured CD4 + T cells and monocytes, comparing patients and controls. Results: Concentrations of IL-26 are higher in serum and induced sputum of asthmatic patients than in HC. Moreover, IL-26 protein and mRNA expression were significantly elevated in asthma sputum cells compared to PBMCs. We observed a positive correlation between body mass index (BMI) and sputum fluid IL-26, while the correlation between IL-26 and lung function tests (FEV1% and FEV1/FVC ratio) was negative. IL-17A was highly expressed in SF and correlated positively with IL-26. In patients' sputum IL-26 and IL-17A were significantly associated with neutrophils. Stimulation of cultured CD4 + T cells with monocytes by recombinant IL-26 promoted the generation of RORγt + Th17 + cells inducing the production of IL-17A, IL-1β, IL-6 and TNF-α cytokines. IL-26 expressed in SF was biologically active and induced IL-17 secretion in the presence of IL-1β and IL-6 cytokines. Conclusion: These findings show that IL-26 is highly produced in asthmatic sputum, induces pro-inflammatory cytokine secretion by monocytes/macrophages, and favours Th17 cell generation. IL-26 thereby appears as a novel pro-inflammatory cytokine, produced locally in the airways that may constitute a promising target to treat asthma inflammatory process.

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Section: Discussionmentioning

confidence: 99%

Sputum IL-26 Is Overexpressed in Severe Asthma and Induces Proinflammatory Cytokine Production and Th17 Cell Generation: A Case–Control Study of Women

Louhaichi¹,

Mlika²,

Hamdi³

et al. 2020

JAA

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“…The participants coughed deep sputum induced by hypertonic saline, and 10 ml peripheral blood was extracted from participants. When we collected the induced sputum, we selected sputum suppository to weigh, add 4 times the weight of 0.1%DTT to dissolve it [ 10 ]. Then the liquid was filtered through the cell sieve.…”

Section: Methodsmentioning

confidence: 99%

Increased expression of IL1-RL1 is associated with type 2 and type 1 immune pathways in asthma

Chen

et al. 2022

BMC Immunol

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Background Asthma is a common chronic airway disease in the world. The purpose of this study was to explore the expression of IL1-RL1 in sputum and its correlation with Th1 and Th2 cytokines in asthma. Methods We recruited 132 subjects, detected IL1-RL1 protein level in sputum supernatant by ELISA, and analyzed the correlation between the expression level of IL1-RL1 and fraction of exhaled nitric oxide (FeNO), IgE, peripheral blood eosinophil count (EOS#), and Th2 cytokines (IL-4, IL-5, IL-10, IL-13, IL-33 and TSLP) and Th1 cytokines (IFN-γ, IL-2, IL-8). The diagnostic value of IL1-RL1 was evaluated by ROC curve. The expression of IL1-RL1 was further confirmed by BEAS-2B cell in vitro. Results Compared with the healthy control group, the expression of IL1-RL1 in sputum supernatant, sputum cells and serum of patients with asthma increased. The AUC of ROC curve of IL1-RL1 in sputum supernatant and serum were 0.6840 (p = 0.0034), and 0.7009 (p = 0.0233), respectively. IL1-RL1 was positively correlated with FeNO, IgE, EOS#, Th2 cytokines (IL-4, IL-5, IL-10, IL-13, IL-33 and TSLP) and Th1 cytokines (IFN-γ, IL-2, IL-8) in induced sputum supernatant. Four weeks after inhaled glucocorticoids (ICS) treatment, the expression of IL1-RL1 in sputum supernatant and serum was increased. In vitro, the expression of IL1-RL1 in BEAS-2B was increased after stimulated by IL-4 or IL-13 for 24 h. Conclusion The expression of IL1-RL1 in sputum supernatant, sputum cells and serum of patients with asthma was increased, and was positively correlated with some inflammatory markers in patients with asthma. IL1-RL1 may be used as a potential biomarker for the diagnosis and treatment of asthma.

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“…The participants coughed deep sputum naturally or were induced by hypertonic saline, and 10 mL of peripheral blood was extracted. The sputum cell pellet was selected, weighed, and added with four times the weight of 0.1% DTT to dissolve [19]. Then, it was filtered by cell sieving.…”

Section: Sample Collectionmentioning

confidence: 99%

Elevated CEACAM5 Levels in Patients with Asthma

Zeng

et al. 2022

Int Arch Allergy Immunol

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Introduction: Asthma is a common chronic respiratory disease. This study aimed to explore the expression level of carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) in induced sputum supernatant, induced sputum cells, and serum of asthma patients. Methods: The protein levels of CEACAM5 in induced sputum supernatant and serum were detected by enzyme-linked immunosorbent assay. The expression of CEACAM5 in induced sputum cells was detected by quantitative reverse transcription polymerase chain reaction (qRT-PCR). We analyzed the correlations between CEACAM5 expression and the clinical characteristics (FeNO and IgE) of asthma. The receiver operating characteristic (ROC) curve was used to analyze the diagnostic value of CEACAM5 in asthma. The expression level of CEACAM5 in 16HBE and BEAS-2B cells was detected by qRT-PCR. Results: The expression of CEACAM5 in induced sputum supernatant, induced sputum cells, and serum of asthma patients was significantly upregulated. Asthma patients with high CEACAM5 expression in induced sputum supernatant had higher levels of FeNO, IgE, and IL-13. The expression levels of CEACAM5 in induced sputum supernatant and induced sputum cells were positively correlated with FeNO and IgE. The ROC curve showed that CEACAM5 had a good diagnostic value in asthma. CEACAM5 expression was upregulated in BEAS-2B and 16HBE cells after IL-4 or IL-13 stimulation for 48 h. Conclusion: The expression levels of CEACAM5 in induced sputum supernatant, induced sputum cells, and serum of asthma patients were significantly increased. CEACAM5 may be involved in eosinophilic inflammation of asthma and may be used as a diagnostic biomarker and therapeutic target of asthma.

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Expression of TSLP and IL-33 receptors on sputum macrophages of asthma patients and healthy subjects

Cited by 14 publications

References 40 publications

<p>Sputum IL-26 Is Overexpressed in Severe Asthma and Induces Proinflammatory Cytokine Production and Th17 Cell Generation: A Case–Control Study of Women</p>

<p>Sputum IL-26 Is Overexpressed in Severe Asthma and Induces Proinflammatory Cytokine Production and Th17 Cell Generation: A Case–Control Study of Women</p>

Increased expression of IL1-RL1 is associated with type 2 and type 1 immune pathways in asthma

Elevated CEACAM5 Levels in Patients with Asthma

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